Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,808,098 T→A 7.4% I50N (ATC→AAC)  kdsA‑I → 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,808,0980TA 7.4% 196.2 / 5.1 68I50N (ATC→AAC) kdsA‑I2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base T (31/32);  new base A (3/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCACGAGC  >  NC_002947/1807955‑1808244
                                                                                                                                               |                                                                                                                                                  
ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCg                                                                                                                                                >  3:244059/1‑148 (MQ=255)
 cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                                              <  3:16142/149‑1 (MQ=255)
 cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                                              <  4:210800/149‑1 (MQ=255)
  agaagaTGATTCTCTTCTGTAACATCGAGATCGCCAACTACAAGCCGTTCGTCCTGTTCGGCTGCATGAACGTTCTGGAATCCCGTGACATGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAATCTCGGTATCCCGTAc                                                                                                                                             <  6:146440/149‑1 (MQ=255)
  agaagaTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                             >  6:97494/1‑149 (MQ=255)
       aTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACgtgt                                                                                                                                         >  3:61861/1‑148 (MQ=255)
        tCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                                                       <  4:102321/149‑1 (MQ=255)
          aTTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                                                       >  1:151801/1‑147 (MQ=255)
              gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                                                  >  5:76166/1‑132 (MQ=255)
              gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                                                  <  6:76166/132‑1 (MQ=255)
                    gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                           <  5:97494/149‑1 (MQ=255)
                    gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                           >  1:219417/1‑149 (MQ=255)
                    gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                           >  7:236863/1‑149 (MQ=255)
                         aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                             <  4:154579/126‑1 (MQ=255)
                         aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                             >  3:154579/1‑126 (MQ=255)
                         aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGAc                                                                                                                        >  4:198191/1‑147 (MQ=255)
                             agaTCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGc                                                                                                                   >  6:241211/1‑148 (MQ=255)
                                    cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                           >  1:144639/1‑149 (MQ=255)
                                    cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                           <  4:75301/149‑1 (MQ=255)
                                    cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                           >  6:316931/1‑149 (MQ=255)
                                    cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                           <  2:272878/149‑1 (MQ=255)
                                    cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                           >  5:242522/1‑149 (MQ=255)
                                     aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                                          <  3:198191/149‑1 (MQ=255)
                                     aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                                          <  7:175694/149‑1 (MQ=255)
                                        gACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtcgtc                                                                                                       <  8:236863/149‑1 (MQ=255)
                                          cAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATGCGGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCACGTAAGTGTTCAAGGACAGTGTCGACAAGGCCAACAGTTCGTCgg                                                                                                     >  7:139001/1‑149 (MQ=255)
                                            aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                                                   <  7:253826/117‑1 (MQ=255)
                                            aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                                                   >  8:253826/1‑117 (MQ=255)
                                             gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTaa                                                                                                  >  4:273114/1‑149 (MQ=255)
                                             gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                                               >  6:91588/1‑103 (MQ=37)
                                             gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                                               <  5:91588/104‑2 (MQ=37)
                                                     tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                                          <  2:158394/149‑1 (MQ=255)
                                                     tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                                          >  2:152458/1‑149 (MQ=255)
                                                      ccTGTTCGGAGGCATGAACGTTCTGGAATCGCGTGACCTGGCGATGAAGGTATGCGAAGAGTACGTGAGGGTTACAGAGAAGCTCGGTATCCCGTACTTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAAAGTCCTaaa                                                                                         >  7:130024/1‑147 (MQ=255)
                                                          ttCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTgg                                                                                     <  1:152458/149‑1 (MQ=255)
                                                                ggcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTTCGAAGAGTACGTGCGGTTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAATGCCAGTTTCGACAAGGCCAACCGTTAGTCGGTAACGTCCTACCGTGGCCCTgg                                                                               <  8:139001/149‑1 (MQ=255)
                                                                 gcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATACCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTgg                                                                               >  7:85134/1‑148 (MQ=255)
                                                                            cTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgg                                                                    <  2:219417/148‑1 (MQ=255)
                                                                             tGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgggg                                                                  >  7:69389/1‑149 (MQ=255)
                                                                              ggAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGc                                                                 >  6:37159/1‑149 (MQ=255)
                                                                                 aTCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                               >  2:148209/1‑148 (MQ=255)
                                                                                    ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                               >  4:306608/1‑145 (MQ=255)
                                                                                    ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                               >  1:207222/1‑145 (MQ=255)
                                                                                         aCCTGGCTAAGAAGGTCTGCGAAGAGTAAGTGCGGGTTACCTAGAAGCTCTGTATACCGTACGTTTTCAATGCCATTTTCGACAATGACAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                       <  8:130024/148‑1 (MQ=255)
                                                                                         aCCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                       <  5:241211/148‑1 (MQ=255)
                                                                                         aCCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                       <  6:242522/148‑1 (MQ=255)
                                                                                              gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                                   <  7:171102/147‑1 (MQ=255)
                                                                                              gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                                   <  2:207222/147‑1 (MQ=255)
                                                                                                 aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                                                >  7:103806/1‑67 (MQ=255)
                                                                                                 aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                                                <  8:103806/67‑1 (MQ=255)
                                                                                                 aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                                   >  1:293905/1‑144 (MQ=255)
                                                                                                       gTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgcg                                        >  2:301062/1‑149 (MQ=255)
                                                                                                            cGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCt                                   >  6:194550/1‑149 (MQ=255)
                                                                                                             gAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCtt                                  >  8:272358/1‑149 (MQ=255)
                                                                                                                 agTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAAc                              <  5:316931/149‑1 (MQ=255)
                                                                                                                    aCGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGt                            <  2:151801/148‑1 (MQ=255)
                                                                                                                         cGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                      <  8:85134/149‑1 (MQ=255)
                                                                                                                         cGGGATACCGAGCTTCTCGGTAACCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                      >  1:131905/1‑149 (MQ=255)
                                                                                                                           ggTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGa                    <  3:28852/149‑1 (MQ=255)
                                                                                                                               aCCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                      >  4:221286/1‑143 (MQ=255)
                                                                                                                               aCCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                      <  3:221286/143‑1 (MQ=255)
                                                                                                                                ccGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCAc                <  3:306608/148‑1 (MQ=255)
                                                                                                                                   agaAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCAGCTTCAACGTGCCGGTGATCACCGAt            <  1:148209/149‑1 (MQ=255)
                                                                                                                                       gCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGc        <  5:194550/149‑1 (MQ=255)
                                                                                                                                        cTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCa       >  6:307859/1‑149 (MQ=255)
                                                                                                                                          cGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCAc      >  3:109253/1‑148 (MQ=255)
                                                                                                                                           ggTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCACg     <  1:301062/148‑1 (MQ=255)
                                                                                                                                             tATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCACGAGc  <  4:61861/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGCACGAGC  >  NC_002947/1807955‑1808244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑