Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,656 C→T 8.5% intergenic (‑98/+34) PP_1639 ← / ← yohC SprT family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,6560CT 8.5% 162.6 / 3.5 58intergenic (‑98/+34)PP_1639/yohCSprT family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (29/24);  new base T (2/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCT  >  NC_002947/1835511‑1835804
                                                                                                                                                 |                                                                                                                                                    
ggCTTGCTGGTAAGAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTg                                                                                                                                                   <  3:175204/149‑1 (MQ=255)
 gCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTgg                                                                                                                                                  >  3:228022/1‑149 (MQ=255)
   ttGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTggct                                                                                                                                                <  4:28463/149‑3 (MQ=255)
     gCTGGTAACAGGTTTCGACGCGTTGTTTGCGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGtt                                                                                                                                                <  3:17080/147‑1 (MQ=255)
      cTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgt                                                                                                                                              <  6:148636/148‑2 (MQ=255)
       tGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATg                                                                                                                                            >  8:175759/1‑149 (MQ=255)
         gttactggTTTCGACGCGTTGTTTGAGCTGCTCGGGCTTGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGtt                                                                                                                                                <  2:309750/137‑1 (MQ=255)
         gTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGtt                                                                                                                                                >  1:309750/1‑143 (MQ=255)
            aCAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGccc                                                                                                                                         >  2:260025/1‑147 (MQ=255)
                gTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAgtgt                                                                                                                                   >  3:217151/1‑149 (MQ=255)
                   tCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgtg                                                                                                                                             <  3:104624/136‑3 (MQ=38)
                   tCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAACCAGGGGCCGCCGCAAGGCGGCCTCTGGCTgtg                                                                                                                                             >  4:104624/1‑134 (MQ=38)
                    cGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTgg                                                                                                                                <  4:262366/148‑1 (MQ=255)
                        gcgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCgg                                                                                                                           >  5:275338/1‑149 (MQ=255)
                         cgTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGt                                                                                                                          <  5:6913/149‑1 (MQ=255)
                              tttGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGt                                                                                                                      <  4:228022/148‑1 (MQ=255)
                                tGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                                    >  7:122874/1‑148 (MQ=255)
                                 gAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTc                                                                                                                    >  3:194343/1‑147 (MQ=255)
                                    ctgctCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGtt                                                                                                               >  6:255178/1‑149 (MQ=255)
                                        tCGGTCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCAGAGTAACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTAGGTTCGGTCTGTTCTAGTTGgtg                                                                                                           >  5:226080/1‑149 (MQ=255)
                                        tCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgtg                                                                                                           <  7:268413/149‑1 (MQ=255)
                                               tGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACgg                                                                                                    <  6:286030/149‑1 (MQ=255)
                                                  cggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgtg                                                                                                           >  8:93909/1‑139 (MQ=255)
                                                  cggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGgtg                                                                                                           <  7:93909/139‑1 (MQ=255)
                                                  cggggcggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGcc                                                                                                 >  5:262887/1‑149 (MQ=255)
                                                        ggTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGc                                                                                           >  7:278833/1‑149 (MQ=255)
                                                                  aCCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTccc                                                                                   <  6:262887/147‑1 (MQ=255)
                                                                  aCCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTccc                                                                                   <  4:217151/147‑1 (MQ=255)
                                                                  aCCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCa                                                                                 <  7:175759/149‑1 (MQ=255)
                                                                     aGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCg                                                                                                                            <  1:309415/103‑1 (MQ=255)
                                                                     aGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCg                                                                                                                            >  2:309415/1‑103 (MQ=255)
                                                                     aGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGa                                                                               >  5:75503/1‑148 (MQ=255)
                                                                       gCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGGCCCAGTGTTGGTTCGGTCTGTTCTAGGTGGTGTAGACGGGGCCCACGCCCACTCCCCAGATac                                                                            >  4:245263/1‑148 (MQ=255)
                                                                       gCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGt                                                                                                                      >  4:293589/1‑107 (MQ=255)
                                                                       gCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGt                                                                                                                      <  3:293589/107‑1 (MQ=255)
                                                                                  cAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGCGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGct                                                                 >  6:250727/1‑148 (MQ=255)
                                                                                       gcAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCAt                                                            >  5:48955/1‑149 (MQ=255)
                                                                                         aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGc                                                                  >  5:82480/1‑141 (MQ=255)
                                                                                         aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGc                                                                  <  6:82480/141‑1 (MQ=255)
                                                                                         aGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGa                                                          >  8:248271/1‑149 (MQ=255)
                                                                                                        tCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACTGTAGCGGCCATGATTGCCACCAGtacta                                           >  6:322059/1‑149 (MQ=255)
                                                                                                        tCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGtacta                                           >  5:94152/1‑149 (MQ=255)
                                                                                                            cGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCaa                                       <  4:194343/149‑1 (MQ=255)
                                                                                                            cGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCaa                                       <  6:75503/149‑1 (MQ=255)
                                                                                                             gttaaGCGCACAGCAAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGACACCATTACTACCAAg                                      <  4:91007/145‑1 (MQ=255)
                                                                                                                 aGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCa                                  >  7:281556/1‑149 (MQ=255)
                                                                                                                      acaGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTa                                                                                                                  <  3:260658/64‑1 (MQ=255)
                                                                                                                      acaGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTa                                                                                                                  >  4:260658/1‑64 (MQ=255)
                                                                                                                       caGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCa                            <  8:122874/149‑1 (MQ=255)
                                                                                                                          ccAGGGGCCGCCGCAAGGCGGCCTCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGGCACCAGTACTACCAAGCCCACTGCCCGCa                         >  3:21992/1‑149 (MQ=255)
                                                                                                                          ccAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCa                         <  6:275338/149‑1 (MQ=255)
                                                                                                                            agagGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCAcc                       >  6:27792/1‑149 (MQ=255)
                                                                                                                                 ccgccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTACCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCt                  >  6:107410/1‑149 (MQ=255)
                                                                                                                                  cgccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCTCCAGTACTACCAAGCCCACTGCCAGCACCGAGCTg                 <  1:260025/149‑1 (MQ=255)
                                                                                                                                  cgccTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCGCCAGTACTACCAAGCCCACTGCCAGCACCGAGCTg                 <  8:278833/149‑1 (MQ=255)
                                                                                                                                     cTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCa                            <  7:294789/135‑1 (MQ=255)
                                                                                                                                     cTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCa                            >  8:294789/1‑135 (MQ=255)
                                                                                                                                           gcggcCCCTGGTTGATGCCCCAGTGTTGGTTCGGTGTGTTCTAGTTGGAGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTTCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAg         >  2:309752/1‑148 (MQ=255)
                                                                                                                                                cccTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGcc   >  3:311315/1‑149 (MQ=255)
                                                                                                                                                 ccTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCt  >  7:119787/1‑149 (MQ=255)
                                                                                                                                                 ccTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTGCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCt  <  8:281556/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGCTTGCTGGTAACAGGTTTCGACGCGTTGTTTGAGCTGCTCGGGCATGGCGGGGCGGTTCTCCTGACCAGGCATTATGCCGCAAGCGCAGGTGGCTTGCCGAGTCACCGGTCAGCGCACAGCCAGAGGCCGCCTTGCGGCGGCCCCTGGTTGATGCCCCAGTGTTGGTTCGGTCTGTTCTAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCT  >  NC_002947/1835511‑1835804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑