Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,926,514 C→A 9.8% D118Y (GAC→TAC) ‡ PP_1726 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,926,5140CA 9.8% 174.2 / 9.0 61D118Y (GAC→TAC) ‡PP_1726ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (31/24);  new base A (2/4);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

TGTACTTGCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGAGCTCATGTC  >  NC_002947/1926370‑1926655
                                                                                                                                                |                                                                                                                                              
tGTACTTGCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑Ag                                                                                                                                           >  6:117788/1‑149 (MQ=255)
 gTACTTGCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑Ag                                                                                                                                           <  7:149445/148‑1 (MQ=255)
       gCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttg                                                                                                                                    >  5:46994/1‑149 (MQ=255)
       gCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttg                                                                                                                                    >  7:168317/1‑149 (MQ=255)
       gCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGGACC‑AGGTgccg                                                                                                                                     <  4:309827/148‑3 (MQ=255)
       gCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGGACC‑AGGTgccg                                                                                                                                     <  4:304854/148‑3 (MQ=255)
       gCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGGACC‑AGGTgcc                                                                                                                                      <  6:317790/147‑2 (MQ=255)
        ccTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttgg                                                                                                                                   >  4:100594/1‑149 (MQ=255)
        ccTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttgg                                                                                                                                   >  2:175955/1‑149 (MQ=255)
               ccAGGTCATGCCAGGTTTTCGGCCGTTACTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGATATGGTGCCGGTATAGTCCAGCGCCCAGTGTCAGTAAATGTCCTTGGACCATTAAAGAACATTGTCCC‑ATGTGCTTGGATTGt                                                                                                                              >  5:172140/1‑147 (MQ=255)
                cAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAg                                                                                                                           >  5:89000/1‑149 (MQ=255)
                cAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAg                                                                                                                           <  2:181818/149‑1 (MQ=255)
                cAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAg                                                                                                                           <  1:175955/149‑1 (MQ=255)
                cAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAg                                                                                                                           >  6:93901/1‑149 (MQ=255)
                   gTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAggct                                                                                                                        <  8:217598/149‑1 (MQ=255)
                    tccggCCCGGTGTTCGGCCGTTCCTCTTCCTTGACCAGGCCCTGGTGGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAggctg                                                                                                                       <  8:168317/145‑1 (MQ=255)
                      aTGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAggctgg                                                                                                                      <  5:117788/148‑1 (MQ=255)
                         ccAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGa                                                                                                                  >  3:257620/1‑149 (MQ=255)
                           aGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACg                                                                                                                >  7:222761/1‑149 (MQ=255)
                                ttgggCCGTTCCTATTCGTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCt                                                                                                            <  3:100594/145‑1 (MQ=255)
                                            tctTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTc                                                                                                       <  3:297165/141‑1 (MQ=255)
                                            tctTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTc                                                                                                       >  4:297165/1‑141 (MQ=255)
                                            tctTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGAt                                                                                               >  2:168394/1‑149 (MQ=255)
                                             ctTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttgg                                                                                                                                   >  1:10596/1‑112 (MQ=255)
                                             ctTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTgcttgg                                                                                                                                   <  2:10596/112‑1 (MQ=255)
                                                ccTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCg                                                                                             <  8:222761/147‑1 (MQ=255)
                                                 cTTGACCCGGGCCTTGATCATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTAGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGc                                                                                          <  4:18754/149‑1 (MQ=255)
                                                          gTCCTTGTTGATGATGAAATCGATTGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAggcgg                                                                                 <  6:172140/149‑1 (MQ=255)
                                                              ttgttgATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCAcc                                                                             >  4:267614/1‑149 (MQ=255)
                                                                          aaaGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGGACC‑AGGTgccg                                                                                                                                     >  3:68055/1‑79 (MQ=255)
                                                                          aaaGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGGACC‑AGGTgccg                                                                                                                                     <  4:68055/81‑3 (MQ=255)
                                                                               gATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCgg                                                            >  6:329386/1‑149 (MQ=255)
                                                                               gATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCgg                                                            >  7:82162/1‑149 (MQ=255)
                                                                                aTGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGgc                                                           <  3:267614/149‑1 (MQ=255)
                                                                                aTGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGgc                                                           <  5:93901/149‑1 (MQ=255)
                                                                                    tGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTg                                                       >  1:61537/1‑149 (MQ=255)
                                                                                    tGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTg                                                       >  7:214530/1‑149 (MQ=255)
                                                                                       cGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCg                                                    <  6:89000/149‑1 (MQ=255)
                                                                                          tataGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTg                                                 <  6:46994/149‑1 (MQ=255)
                                                                                               gCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCtt                                            >  5:261548/1‑149 (MQ=255)
                                                                                                 cAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTtct                                          >  7:60517/1‑149 (MQ=255)
                                                                                                  aGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTtctc                                         <  8:82162/149‑1 (MQ=255)
                                                                                                     gcCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTc                                                              >  4:131601/1‑125 (MQ=255)
                                                                                                     gcCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTc                                                              <  3:131601/125‑1 (MQ=255)
                                                                                                        cAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTc                                   >  7:506/1‑149 (MQ=255)
                                                                                                              ccGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCCTAGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACt                              >  1:58350/1‑149 (MQ=255)
                                                                                                                    ttgtccttgGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAg                       >  5:298306/1‑149 (MQ=255)
                                                                                                                      gtccttgGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAg                       <  6:298306/147‑1 (MQ=255)
                                                                                                                        ccttgGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTcc                   >  3:121223/1‑149 (MQ=255)
                                                                                                                         cttgGCCCTTTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCt                  >  2:111157/1‑149 (MQ=255)
                                                                                                                            ggCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTggg               >  6:18180/1‑149 (MQ=255)
                                                                                                                            ggCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTgg                <  5:329386/148‑1 (MQ=255)
                                                                                                                             gCCCTTTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTggg               <  1:111157/148‑1 (MQ=255)
                                                                                                                               ccATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGcc             >  1:195963/1‑148 (MQ=255)
                                                                                                                               ccATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCg            <  8:214530/149‑1 (MQ=255)
                                                                                                                                cATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGa           <  1:168394/149‑1 (MQ=255)
                                                                                                                                 aTTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCtt                                    <  3:138577/123‑1 (MQ=255)
                                                                                                                                 aTTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCtt                                    >  4:138577/1‑123 (MQ=255)
                                                                                                                                       gCACCTGGGACC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGAGCTCATg    >  8:113851/1‑149 (MQ=255)
                                                                                                                                        cACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGAGCTCATGt   >  2:166913/1‑149 (MQ=255)
                                                                                                                                         aCCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGAGCTCATGTc  >  5:126740/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TGTACTTGCCTTTCTCCAGGTCATGCCAGGTTTTCGGCCGTTCCTCTTCCTTGACCAGGTCCTTGTTGATGATGAAAGCGATGGTGCCGGTATAGGCCAGCGCCCAGTGGCCGTCCTTGTCCTTGGCCCATTCCGGCACCTGGTCCC‑AGGTGCTTGGCTTGTAAGGCTGGCTGACGCCCTTGGTCACCGCGATCGGGCCGAAGGCGGCACCCACGTCACCGATGTCGGCGCTGGCGTTGTCCTTCTCGGCTTCGAACTTGGCCAGTTCCTGGGCCGAGCTCATGTC  >  NC_002947/1926370‑1926655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑