Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,933,669 G→C 6.7% L29V (CTG→GTG)  minD ← MinC‑MinD‑MinE system ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,933,6690GC 6.7% 194.8 / 4.1 60L29V (CTG→GTG) minDMinC‑MinD‑MinE system ATPase
Reads supporting (aligned to +/- strand):  ref base G (31/25);  new base C (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GTCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAA  >  NC_002947/1933523‑1933813
                                                                                                                                                   |                                                                                                                                                 
gTCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTg                                                                                                                                                 >  2:251808/1‑149 (MQ=255)
   cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGc                                                                                                                                                >  5:190046/1‑147 (MQ=255)
   cTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGa                                                                                                                                              <  6:153555/149‑1 (MQ=255)
    ttGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TCCACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCGTGTGGCCGCGCAGTGCGAg                                                                                                                                             >  4:234307/1‑149 (MQ=255)
       aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGcc                                                                                                                                          <  5:199870/149‑1 (MQ=255)
       aTCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGc                                                                                                                                           <  8:296952/148‑1 (MQ=255)
        tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCg                                                                                                                                         >  7:119313/1‑149 (MQ=255)
        tCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCg                                                                                                                                         >  3:102548/1‑149 (MQ=255)
          aGGGCCTGTTGCAGGTGGGCTTCGCCGGTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCgg                                                                                                                                        <  6:195264/148‑1 (MQ=255)
                         ttGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCt                                                                                                                       <  6:321570/149‑1 (MQ=255)
                         ttGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCt                                                                                                                       <  6:218306/149‑1 (MQ=255)
                             cTTCGCCGTTGACCACGTTGACGAAGTCGTTACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTgg                                                                                                                     >  1:195236/1‑148 (MQ=255)
                             cTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTa                                                                                                                   <  7:80531/149‑1 (MQ=255)
                                  ccGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGt                                                                                                              >  8:7699/1‑149 (MQ=255)
                                     ttGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCtt                                                                                                           <  3:248626/149‑1 (MQ=255)
                                       gACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTg                                                                                                          <  8:119313/148‑1 (MQ=255)
                                       gACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTg                                                                                                          <  4:102548/148‑1 (MQ=255)
                                        aCCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGcc                                                                                                        >  2:203587/1‑149 (MQ=255)
                                             gTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCacac                                                                                                   >  2:154441/1‑149 (MQ=255)
                                                   gAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGcc                                                                                                        >  8:257757/1‑138 (MQ=255)
                                                   gAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGcc                                                                                                        <  7:257757/138‑1 (MQ=255)
                                                   gAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCt                                                                                             >  6:56071/1‑149 (MQ=255)
                                                   gAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGCGGCCTCGCACTGCGCGGccacaa                                                                                                                                      >  4:210569/1‑105 (MQ=255)
                                                   gAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGCGGCCTCGCACTGCGCGGccacaa                                                                                                                                      <  3:210569/109‑5 (MQ=255)
                                                         g‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCg                                                                                        <  5:56071/148‑1 (MQ=255)
                                                             caccacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAg                                                                                    >  7:13554/1‑149 (MQ=255)
                                                                cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGgcgg‑cg                                                                                                                         >  7:268833/1‑109 (MQ=255)
                                                                cacGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGgcgg‑cg                                                                                                                         <  8:268833/109‑1 (MQ=255)
                                                                                cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGaa                                                                        >  2:228342/1‑142 (MQ=255)
                                                                                cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                                 <  6:190046/149‑1 (MQ=255)
                                                                                cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGc                                                                 >  6:192742/1‑149 (MQ=255)
                                                                                cccATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGCTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGaa                                                                        <  1:228342/142‑1 (MQ=255)
                                                                                  cATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGcc                                                                >  8:110327/1‑148 (MQ=255)
                                                                                   aTGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                     >  6:62797/1‑126 (MQ=255)
                                                                                   aTGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGaa                                                                                     <  5:62797/126‑1 (MQ=255)
                                                                                   aTGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGcc                                                                >  6:3467/1‑147 (MQ=255)
                                                                                       tCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCAAACACCCCCCTTGCCGGAAGTAAACACGAGAATCTTGGCCAAGgtg                                                          >  8:262228/1‑149 (MQ=255)
                                                                                          ggTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGttt                                                       <  1:251808/149‑1 (MQ=255)
                                                                                            tCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGT‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACAGCGAGAATCTTGGCCAAGGTGTTTCa                                                     >  4:166556/1‑149 (MQ=255)
                                                                                               aGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAccc                                                  <  2:195236/149‑1 (MQ=255)
                                                                                                      gCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGAAACCACCAGAATCTTGGCAAAGGTGTTTCACCCCTaaaaa                                           >  4:181003/1‑149 (MQ=255)
                                                                                                        aGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATa                                         >  7:105118/1‑149 (MQ=255)
                                                                                                            ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCGAg                                                             <  4:289061/125‑1 (MQ=255)
                                                                                                            ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCGAg                                                             >  3:289061/1‑125 (MQ=255)
                                                                                                            ccACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCg                                     <  1:154441/149‑1 (MQ=255)
                                                                                                             cACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCTAGGCATTTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCgg                                    <  7:262228/149‑1 (MQ=255)
                                                                                                               cGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGa                                  <  7:7699/149‑1 (MQ=255)
                                                                                                                            aaTGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTg                      >  4:216541/1‑148 (MQ=255)
                                                                                                                              tGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGGCCGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCtcc                     >  1:72643/1‑146 (MQ=255)
                                                                                                                              tGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCa                    >  1:57451/1‑148 (MQ=255)
                                                                                                                              tGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCa                    >  7:85290/1‑148 (MQ=255)
                                                                                                                                aCGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaa                  >  1:327561/1‑148 (MQ=255)
                                                                                                                                  ggTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaaaaa               <  2:19132/149‑1 (MQ=255)
                                                                                                                                  ggTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCaaaaaa               <  3:181003/149‑1 (MQ=255)
                                                                                                                                       tgtgGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGa           >  8:274695/1‑148 (MQ=255)
                                                                                                                                        gtgGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaaa         >  2:43253/1‑149 (MQ=255)
                                                                                                                                         cggCCTCGCACTGCGCGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGaaa         >  5:21489/2‑148 (MQ=255)
                                                                                                                                           gCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCCCACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAagag      >  7:293775/1‑149 (MQ=255)
                                                                                                                                            ccGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCAcccc                                                 <  8:41439/105‑1 (MQ=255)
                                                                                                                                                gcACTGCGCGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCtaa  <  6:21489/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTCCTTGATCAGGGCCTGTTGCAGGTTGGCTTCGCCGTTGACCACGTTGACGAAGTCG‑TACACCACGCGGCGTTCGCAGCCCATGATCAGGTCGAGGTTACGCAGGCCCACGTCGAAGTCGACAATGACGGTCTTGTGGCCGCGCAGTGCGAGGCCGGTACCAATGGCGG‑CGCTGGTAGTGGTCTTGCCCACACCCCCCTTGCCGGAAGTAACCACGAGAATCTTGGCCAAGGTGTTTCACCCCTAAAAATAGTCGGGACACTTGTCCCTGCAAAAAACTGAAATAGCTAA  >  NC_002947/1933523‑1933813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑