Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,023,495	T→C	7.6%	F176S (TTC→TCC)	rmd →	oxidoreductase Rmd

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,023,495	0	T	C	7.6%	181.6 / 4.0	65	F176S (TTC→TCC)	rmd	oxidoreductase Rmd
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base C (3/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGC  >  NC_002947/2023348‑2023637                                                                                                                                                    |                                                                                                                                                 tGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTc                                                                                                                                                 <  2:249655/149‑1 (MQ=255)    tcGAGCAGACCCAGCCAGCGGCGGCCTATGATTACGCGGTCAGCAAGTTGGCCATTGAATACATGGCGCGTCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCctg                                                                                                                                              <  7:262678/148‑1 (MQ=255)           gACCCCGCCAGCGTCGGCCAATGATTACGCGGTCAGCAAGTTTGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCa                                                                                                                                     <  3:90532/149‑1 (MQ=255)            aCCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGccc                                                                                                                                      >  8:58302/1‑147 (MQ=255)              cccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGa                                                                                                                                  <  3:45728/149‑1 (MQ=255)               ccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGa                                                                                                                                  >  4:45728/1‑148 (MQ=255)                   cAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGtt                                                                                                                             >  1:268312/1‑149 (MQ=255)                     gcgcCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGTAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTc                                                                                                                           <  1:44834/149‑1 (MQ=255)                                  aTTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTcgccgc                                                                                                              <  2:268312/149‑1 (MQ=255)                                       gcgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                         >  5:82362/1‑149 (MQ=255)                                       gcgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                         >  5:77646/1‑149 (MQ=255)                                       gcgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                         >  5:123477/1‑149 (MQ=255)                                        cgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CATGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                         <  1:43527/149‑1 (MQ=255)                                         ggTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGcc                                                                                                        >  8:103440/1‑148 (MQ=255)                                             aGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTGATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTc                                                                                                                                                 <  6:90189/105‑1 (MQ=255)                                             aGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTc                                                                                                                                                 >  5:90189/1‑105 (MQ=255)                                                    gcgCCATGTATTCCATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAg                                                                                            >  6:205426/3‑149 (MQ=255)                                                       ccATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCC‑AGGTTGTTTGGGATTTTAGCCGCAAGGCCGACCACATAGAGCtag                                                                                        >  4:269557/1‑147 (MQ=255)                                                        cATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                        <  7:219739/149‑1 (MQ=255)                                                        cATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                        <  5:52201/149‑1 (MQ=255)                                                            gAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCaa                                                                                    >  1:88122/1‑149 (MQ=255)                                                             aaTACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAAc                                                                                   <  7:103440/149‑1 (MQ=255)                                                               tACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCt                                                                                 <  2:207146/149‑1 (MQ=255)                                                                       gcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTAt                                                                         <  7:58302/149‑1 (MQ=255)                                                                           cTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGc                                                                                      >  4:64568/1‑132 (MQ=255)                                                                           cTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGc                                                                                      <  3:64568/132‑1 (MQ=255)                                                                              tggtgTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGa                                                                  >  2:153468/1‑149 (MQ=255)                                                                               ggtgTGACCGTTTGCCTATCGTCATCACGCCTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCAAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACAACATc                                                                                               >  5:288641/1‑119 (MQ=255)                                                                               ggtgTGACCGTTTGCCTATAGTCATCACGCGTGATTTCAATTACACCGTTGTTGGGCAGGCAGATAATTTCATT‑C‑TTCCCAAGATTGTTTCGCATTTTCGCCTCAAGGCATACACCATc                                                                                               <  6:288641/119‑1 (MQ=255)                                                                                   tgACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCa                                                             <  6:123477/149‑1 (MQ=255)                                                                                     aCCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGc                                                           <  2:88122/149‑1 (MQ=255)                                                                                       cGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGa                                                         >  1:193131/1‑149 (MQ=255)                                                                                         tttGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATg                                                       <  6:77646/149‑1 (MQ=255)                                                                                         tttGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATg                                                       <  6:82362/149‑1 (MQ=255)                                                                                             ccTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCATCGATGTACg                                                   >  1:86247/1‑149 (MQ=255)                                                                                              cTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGt                                                  >  1:291956/1‑149 (MQ=255)                                                                                                aTCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               >  4:8635/1‑102 (MQ=255)                                                                                                aTCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                               <  3:8635/102‑1 (MQ=255)                                                                                                aTCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGATCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGc                                                >  4:57126/1‑149 (MQ=255)                                                                                                 tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTg                                                 <  1:1932/147‑1 (MQ=255)                                                                                                    tcatcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCa                                                             <  5:161357/132‑1 (MQ=255)                                                                                                    tcatcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCa                                                             >  6:161357/1‑132 (MQ=255)                                                                                                        caCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAg                                                                                                           <  8:37076/82‑1 (MQ=255)                                                                                                        caCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTg                                         >  4:159624/1‑148 (MQ=255)                                                                                                         aCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCa                                       <  1:153468/149‑1 (MQ=255)                                                                                                             gTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                                   >  6:108694/1‑149 (MQ=255)                                                                                                                 tttCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTAcc                               >  4:216835/1‑149 (MQ=255)                                                                                                                  ttCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                                   >  6:70557/1‑144 (MQ=255)                                                                                                                  ttCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                                   <  5:70557/144‑1 (MQ=255)                                                                                                                    cAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACcgcg                            >  3:236468/1‑149 (MQ=255)                                                                                                                         acacCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAAGCTAGACGTATGGCGCGATTTCATCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCtt                        <  2:86247/148‑1 (MQ=255)                                                                                                                           acCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCgg                           <  8:173543/143‑1 (MQ=255)                                                                                                                           acCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCgg                           >  7:173543/1‑143 (MQ=255)                                                                                                                            acaatcttGGGCA‑GCAGGAAATTCTCTGCC‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATc                    >  4:232717/7‑149 (MQ=38)                                                                                                                             gggTGTGGGCCAGGAATATAATTCCCTC‑C‑TG‑CCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGa                  <  3:269557/148‑1 (MQ=255)                                                                                                                             cGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACAATCGAGCTCGGCAACCTAGACGTATGGCGCGAATTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATc                    >  8:199227/1‑148 (MQ=255)                                                                                                                               gtgtTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAg                 <  3:159624/149‑1 (MQ=255)                                                                                                                               gtgtTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAg                 >  6:75180/1‑149 (MQ=255)                                                                                                                                tcttGGGCA‑GCAGGAAATTCTCTGCC‑TGCCCAAGATTGTTTCGCATTTTCGCCGGAAGGCCGCCACCATCGAGCTCGGCAACCTAGACGGATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGAGCGAgg                >  4:210273/3‑149 (MQ=255)                                                                                                                                tcttGGGCA‑GCAGGAAATTCTCTGCC‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCtt                        <  7:277694/139‑1 (MQ=255)                                                                                                                                tcttGGGCA‑GCAGGAAATTCTCTGCC‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCtt                        >  8:277694/3‑141 (MQ=255)                                                                                                                                       caggcagAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGccc         <  2:291956/149‑1 (MQ=255)                                                                                                                                            agagAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTgg    <  7:138038/149‑1 (MQ=255)                                                                                                                                              agaATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGc  <  2:193131/149‑1 (MQ=255)                                                                                                                                              agaATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGc  >  2:66032/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑C‑TGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGCTGGGC  >  NC_002947/2023348‑2023637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑