Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,031,370 T→G 14.3% intergenic (+43/+32) PP_1806 → / ← kdsA‑II arabinose 5‑phosphate isomerase/2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,031,3700TG14.3% 103.0 / 10.3 42intergenic (+43/+32)PP_1806/kdsA‑IIarabinose 5‑phosphate isomerase/2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base G (4/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

AAGCTTACATGCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCT  >  NC_002947/2031224‑2031517
                                                                                                                                                  |                                                                                                                                                   
aaGCTTACATGCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGt                                                                                                                                                   >  5:44933/1‑149 (MQ=255)
          gCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCgg                                                                                                                                         >  5:325881/1‑149 (MQ=255)
                   cAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCa                                                                                                                                >  5:248496/1‑149 (MQ=255)
                           gTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGc                                                                                                                                        >  1:158956/1‑133 (MQ=255)
                           gTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGc                                                                                                                                        <  2:158956/133‑1 (MQ=255)
                                  ggCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTa                                                                                                                   <  8:127916/147‑1 (MQ=255)
                                   gCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAgg                                                                                                                 <  5:90900/148‑1 (MQ=255)
                                      gtggtCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCaa                                                                                                              >  1:142067/1‑148 (MQ=255)
                                             acgaTTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAAt                                                                                                      >  3:154304/1‑149 (MQ=255)
                                               gaTTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTg                                                                                                    >  6:199693/1‑149 (MQ=255)
                                                   gCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCgg                                                                                                 <  2:12771/148‑1 (MQ=255)
                                                         cTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGGAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAt                                                                                          <  6:248496/149‑1 (MQ=255)
                                                                     tcgtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatca                                                                              >  3:291297/1‑149 (MQ=255)
                                                                       gtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatcatc                                                                            >  2:62529/1‑149 (MQ=255)
                                                                       gtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatcatc                                                                            >  8:283803/1‑149 (MQ=255)
                                                                        tcgAGATATTTGACGGCAATCCAGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGAAATAGTCAATTGCGTGAATGATTTTACAAGatcatc                                                                            >  8:129572/1‑148 (MQ=255)
                                                                          gagaTTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCa                                                                          <  4:291297/148‑1 (MQ=255)
                                                                              tttttGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCt                                                                     <  1:322692/149‑1 (MQ=255)
                                                                                tttGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCtt                                                                    <  4:154304/148‑1 (MQ=255)
                                                                                  tGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTg                                                                  <  2:142067/148‑1 (MQ=255)
                                                                                     cGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGAttt                                                              <  6:44933/149‑1 (MQ=255)
                                                                                      ggCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtg                                                             >  5:228278/1‑149 (MQ=255)
                                                                                         aaTCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGc                                                          <  4:292199/149‑1 (MQ=255)
                                                                                                tAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAtttt                                                                                       >  5:227626/1‑113 (MQ=255)
                                                                                                tAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAtttt                                                                                       <  6:227626/113‑1 (MQ=255)
                                                                                                  gCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAgg                                                  <  7:283803/148‑1 (MQ=255)
                                                                                                   cGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGt                                                <  6:325881/149‑1 (MQ=255)
                                                                                                                 tAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCaa                                  <  6:228278/149‑1 (MQ=255)
                                                                                                                  aaTTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAAt                                 >  8:102682/1‑149 (MQ=255)
                                                                                                                          ttGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTgc                         >  2:311191/1‑149 (MQ=255)
                                                                                                                          ttGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTgc                         <  1:325236/149‑1 (MQ=255)
                                                                                                                                     ggCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCAt              <  7:102682/149‑1 (MQ=255)
                                                                                                                                       aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGc                                          >  7:241035/2‑119 (MQ=255)
                                                                                                                                       aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGc                                          <  8:241035/118‑1 (MQ=255)
                                                                                                                                       aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATca            >  1:10138/2‑149 (MQ=255)
                                                                                                                                        aGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATca            >  8:232606/1‑148 (MQ=255)
                                                                                                                                         gCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATcaca          <  7:232606/149‑1 (MQ=255)
                                                                                                                                          ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtgt                                                            <  3:145866/98‑1 (MQ=255)
                                                                                                                                          ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtgt                                                            >  4:145866/1‑98 (MQ=255)
                                                                                                                                          ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac         >  6:70249/1‑149 (MQ=255)
                                                                                                                                          ccGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac         >  7:148509/1‑149 (MQ=255)
                                                                                                                                           cGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGAAATAGTCAATTGCGGGAATGATTTTACCAGATCATAGACCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac         <  7:129572/148‑1 (MQ=255)
                                                                                                                                                 ttGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCt  >  7:298013/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AAGCTTACATGCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCT  >  NC_002947/2031224‑2031517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑