Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,031,374 C→A 13.3% intergenic (+47/+28) PP_1806 → / ← kdsA‑II arabinose 5‑phosphate isomerase/2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,031,3740CA13.3% 120.5 / 9.1 45intergenic (+47/+28)PP_1806/kdsA‑IIarabinose 5‑phosphate isomerase/2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base C (19/20);  new base A (4/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCTGAT  >  NC_002947/2031234‑2031520
                                                                                                                                            |                                                                                                                                                  
gCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCgg                                                                                                                                            >  5:325881/1‑149 (MQ=255)
         cAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCa                                                                                                                                   >  5:248496/1‑149 (MQ=255)
                 gTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGc                                                                                                                                           >  1:158956/1‑133 (MQ=255)
                 gTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGc                                                                                                                                           <  2:158956/133‑1 (MQ=255)
                        ggCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTa                                                                                                                      <  8:127916/147‑1 (MQ=255)
                         gCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAgg                                                                                                                    <  5:90900/148‑1 (MQ=255)
                            gtggtCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCaa                                                                                                                 >  1:142067/1‑148 (MQ=255)
                                   acgaTTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAAt                                                                                                         >  3:154304/1‑149 (MQ=255)
                                     gaTTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTg                                                                                                       >  6:199693/1‑149 (MQ=255)
                                         gCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCgg                                                                                                    <  2:12771/148‑1 (MQ=255)
                                               cTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGGAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAt                                                                                             <  6:248496/149‑1 (MQ=255)
                                                           tcgtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatca                                                                                 >  3:291297/1‑149 (MQ=255)
                                                             gtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatcatc                                                                               >  8:283803/1‑149 (MQ=255)
                                                             gtcgAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGatcatc                                                                               >  2:62529/1‑149 (MQ=255)
                                                              tcgAGATATTTGACGGCAATCCAGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGAAATAGTCAATTGCGTGAATGATTTTACAAGatcatc                                                                               >  8:129572/1‑148 (MQ=255)
                                                                gagaTTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCa                                                                             <  4:291297/148‑1 (MQ=255)
                                                                    tttttGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCt                                                                        <  1:322692/149‑1 (MQ=255)
                                                                      tttGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCtt                                                                       <  4:154304/148‑1 (MQ=255)
                                                                        tGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTg                                                                     <  2:142067/148‑1 (MQ=255)
                                                                           cGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGAttt                                                                 <  6:44933/149‑1 (MQ=255)
                                                                            ggCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtg                                                                >  5:228278/1‑149 (MQ=255)
                                                                               aaTCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGc                                                             <  4:292199/149‑1 (MQ=255)
                                                                                      tAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAtttt                                                                                          >  5:227626/1‑113 (MQ=255)
                                                                                      tAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGAtttt                                                                                          <  6:227626/113‑1 (MQ=255)
                                                                                        gCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAgg                                                     <  7:283803/148‑1 (MQ=255)
                                                                                         cGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGt                                                   <  6:325881/149‑1 (MQ=255)
                                                                                                       tAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCaa                                     <  6:228278/149‑1 (MQ=255)
                                                                                                        aaTTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAAt                                    >  8:102682/1‑149 (MQ=255)
                                                                                                                ttGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTgc                            >  2:311191/1‑149 (MQ=255)
                                                                                                                ttGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTgc                            <  1:325236/149‑1 (MQ=255)
                                                                                                                           ggCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCAt                 <  7:102682/149‑1 (MQ=255)
                                                                                                                             aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGc                                             >  7:241035/2‑119 (MQ=255)
                                                                                                                             aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGc                                             <  8:241035/118‑1 (MQ=255)
                                                                                                                             aaGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATca               >  1:10138/2‑149 (MQ=255)
                                                                                                                              aGCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATca               >  8:232606/1‑148 (MQ=255)
                                                                                                                               gCCGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATcaca             <  7:232606/149‑1 (MQ=255)
                                                                                                                                ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtgt                                                               <  3:145866/98‑1 (MQ=255)
                                                                                                                                ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTtgt                                                               >  4:145866/1‑98 (MQ=255)
                                                                                                                                ccGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac            >  6:70249/1‑149 (MQ=255)
                                                                                                                                ccGCTTCTGGACAAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac            >  7:148509/1‑149 (MQ=255)
                                                                                                                                 cGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGAAATAGTCAATTGCGGGAATGATTTTACCAGATCATAGACCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCacac            <  7:129572/148‑1 (MQ=255)
                                                                                                                                       ttGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCt     >  7:298013/1‑149 (MQ=255)
                                                                                                                                         gTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCt     <  5:70249/147‑1 (MQ=255)
                                                                                                                                         gTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCTGa   >  3:259137/1‑149 (MQ=255)
                                                                                                                                         gTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTACAATGTCAGTGATCTCGGGCAATCACACTTGGCCTGa   >  2:155201/1‑149 (MQ=255)
                                                                                                                                            cAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCTGAt  >  4:150501/1‑147 (MQ=255)
                                                                                                                                            cAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCTGAt  >  8:22658/1‑147 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GCTGGAGAACAAAATCCGTGCCTTGGCGGTGGTCGACGATTGCGGGGCTATTGTTGGCGTCGTCGAGATTTTTGACGGCAATCCCGTAGCGTGAAACAGCCGGTAATTATCGTTGCCTGGGTAGGCAGCCGCTTCTTGTCCAGAAGCGGCTTTACTCACCGTCGTAGATTAGGCAATAGTCAATTGCGGGAATGATTTTACCAGATCATCCAGCGCTTTGATTTGTGCCAGGAAGGGTTCCAGCTTGTCCAATGGCAGTGCGCTCGGGCCATCACACTTGGCCTGAT  >  NC_002947/2031234‑2031520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑