Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,037,459 T→C 9.2% intergenic (+53/+146) PP_1810 → / ← PP_5478 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,037,4590TC 9.2% 140.7 / 5.1 54intergenic (+53/+146)PP_1810/PP_5478hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base C (2/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

AAACGCTCGGGGCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCTGGTCTCG  >  NC_002947/2037311‑2037593
                                                                                                                                                    |                                                                                                                                      
aaaCGCTCGGGGCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGt                                                                                                                                        >  3:273147/1‑149 (MQ=255)
 aaCGCTCGGGGCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTg                                                                                                                                       >  7:91819/1‑149 (MQ=255)
        ggggCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCACTTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCACCGCCGgtgc                                                                                                                                <  3:108198/149‑2 (MQ=255)
           gCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGACACCGGCACCGCCGgtgccat                                                                                                                             <  7:90311/149‑5 (MQ=255)
           gCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGCATGGATGGCACCGGCACCGCCGgtgccat                                                                                                                             <  1:22138/149‑5 (MQ=255)
            cAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGTAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTcgc                                                                                                                            <  2:237577/149‑1 (MQ=255)
              gagCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCgg                                                                                                                          <  5:78025/149‑1 (MQ=255)
              gagCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCgg                                                                                                                          >  6:269445/1‑149 (MQ=255)
               agCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCggg                                                                                                                         >  7:182244/1‑149 (MQ=255)
                     cgGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGcc                                                                                                                   <  1:65596/149‑1 (MQ=255)
                         tGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGcg                                                                                                               <  1:38905/149‑2 (MQ=255)
                          gAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTc                                                                                                              >  4:53323/1‑149 (MQ=255)
                           aaGGTGTATGATGGGGATGCAATTGCCATCTCGTCGTTTATCATTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGcgaa                                                                                                             <  6:277061/149‑4 (MQ=255)
                           aaGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGGGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGcga                                                                                                              <  6:316313/148‑3 (MQ=255)
                            aGGTGGTTGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGTATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTccc                                                                                                            <  3:240964/149‑1 (MQ=255)
                                ggATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAg                                                                                                        >  3:97037/1‑149 (MQ=255)
                                    gatgGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGtgtg                                                                                                    >  2:68490/1‑149 (MQ=255)
                                     atgGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGtgtg                                                                                                    <  3:248925/148‑1 (MQ=255)
                                      tgGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTggg                                                                                                  <  5:269445/149‑1 (MQ=255)
                                      tgGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTggg                                                                                                  <  6:268018/149‑1 (MQ=255)
                                        gggATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTgggg                                                                                                 <  3:236367/148‑1 (MQ=255)
                                               aaTTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGc                                                                                         <  8:182244/149‑1 (MQ=255)
                                                      aTCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCa                                                                                  <  3:95435/149‑1 (MQ=255)
                                                       tctcGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAg                                                                                 >  2:53462/1‑149 (MQ=255)
                                                         tcGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTg                                                                               >  7:210534/1‑149 (MQ=255)
                                                           ggCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACGGTGTGGGGTGCTTGGCCAATGCAGTgg                                                                              <  3:53323/148‑1 (MQ=255)
                                                           ggCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGt                                                                             <  1:68490/149‑1 (MQ=255)
                                                           ggCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATCGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGt                                                                             <  6:267056/149‑1 (MQ=255)
                                                                       tgtTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCa                                                                 <  1:187662/149‑1 (MQ=255)
                                                                            aTCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGACCGCTCCCACATTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCt                                                            >  5:97764/1‑149 (MQ=255)
                                                                               cgcgCAGTGAGATTTGAGCGGCGGCTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGCGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTggg                                                         >  5:269442/1‑148 (MQ=255)
                                                                                 cgcAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCt                                                       <  8:91819/149‑1 (MQ=255)
                                                                                      tGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCtgatg                                                   >  2:10451/1‑148 (MQ=255)
                                                                                          aTTTGAGCGGCGGGTGGGGTCTGTGATCTTGTAGGCATGGGTTGGATCGCACCGGCGGTGCCGGTGTTCGCGGGCGCGGCCGCTCCCACAGTGCGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGc                                               <  6:269442/148‑1 (MQ=255)
                                                                                               aGCGGCGGGTGGGGGCTGTGATCTTGTAGTCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCAtt                                          >  6:285438/1‑148 (MQ=255)
                                                                                                       gTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTg                                   >  4:140654/1‑147 (MQ=255)
                                                                                                       gTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTg                                   <  3:140654/147‑1 (MQ=255)
                                                                                                           gggCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGc                              >  4:90458/1‑148 (MQ=255)
                                                                                                           gggCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCa                             <  8:210534/149‑1 (MQ=255)
                                                                                                               tgtgATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGc                          >  7:180593/1‑148 (MQ=255)
                                                                                                                  gATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAgg                      >  8:181112/1‑149 (MQ=255)
                                                                                                                   aTCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAgg                      >  8:164613/1‑148 (MQ=255)
                                                                                                                   aTCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAgg                      >  7:225434/1‑148 (MQ=255)
                                                                                                                        gTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCa                    <  1:53462/145‑1 (MQ=255)
                                                                                                                           ggCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTc             <  3:90458/149‑1 (MQ=255)
                                                                                                                            gCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCg            >  2:119720/1‑149 (MQ=255)
                                                                                                                              aTGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCCCAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCCCGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCACGCGAGGCATAGCCTCGcc          >  3:162103/1‑149 (MQ=255)
                                                                                                                               tGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCt         >  6:68068/1‑149 (MQ=255)
                                                                                                                                  gatggatgGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCTGGt      <  5:285438/149‑1 (MQ=255)
                                                                                                                                    tggatggCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTAGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCTATGCCTCGCTTgcagt    <  4:273147/149‑5 (MQ=38)
                                                                                                                                     ggatggCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGc                                                         >  6:41846/1‑95 (MQ=255)
                                                                                                                                     ggatggCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGc                                                         <  5:41846/95‑1 (MQ=255)
                                                                                                                                     caccggCACCGCCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCTGGtctc   >  3:78308/5‑149 (MQ=255)
                                                                                                                                      gcgaacACCGGCACCGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCTGGTCTcg  >  4:251106/6‑149 (MQ=255)
                                                                                                                                      gcgaacACCGGCACCGCCGGTGTTCGCGGGCGCGCCCGCTACCACAGTGTGGGGTGCTTTGCCAATGAAGTTGTTTGCCCTCGCCACGTCTGGCCTGATTAAGCCCATTTATTATGACTGCAAACGACGCATAGCCACGCCTGCTCTcg  >  8:259746/6‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
AAACGCTCGGGGCAGAGCCCGCGGGTGAAGGTGGATGATGGGGATGCAATTGCCATCTCGGCGTTTATCAGTGTTTATCCGCGCAGTGAGATTTGAGCGGCGGGTGGGGGCTGTGATCTTGTAGGCATGGGATGGATGGCACCGGCGGTGCCGGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTGGGGTGCTTGGCCAATGCAGTGGTTTGCCCTCGCCACGTCTGGCCTGATGAAGCCCATTTCTTCTGACTGCAAGCGAGGCATAGCCTCGCCTGGTCTCG  >  NC_002947/2037311‑2037593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑