Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,068,152	A→T	9.8%	intergenic (‑97/+24)	plsC ← / ← PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,068,152	0	A	T	9.8%	137.8 / 5.4	51	intergenic (‑97/+24)	plsC/PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base T (3/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

AACGGCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCCGCGCTGCG  >  NC_002947/2068005‑2068299                                                                                                                                                     |                                                                                                                                                    aaCGGCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGAATCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTCTTACGGCCC‑TTTCGCGGGCAAg                                                                                                                                                    >  1:17618/1‑149 (MQ=255)         gAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCCTTTTCGCGGGCTTGCCCGCGa                                                                                                                                             <  4:312010/149‑1 (MQ=255)           aaaTGCAGCCCAAGCAGGAACATACGCAATGAATAACGCATGGTACGACTCACACCAGACGGTCGCGGGTCAGTGTACGTGTGTGCACTGGCTGGGGCAAAGCTTGTTTCAGGCCTGTTACGGCGC‑TTTCGCGGGCTTGCCCGCGaaa                                                                                                                                           <  4:47617/148‑1 (MQ=255)                 aGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGccc                                                                                                                                    >  8:64934/1‑149 (MQ=255)                   ccccAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGt                                                                                                                                  <  2:115994/149‑1 (MQ=255)                     ccAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGc                                                                                                                                <  1:144336/149‑1 (MQ=255)                          atctaCATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGGGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTa                                                                                                                           <  7:272440/145‑1 (MQ=255)                          agtaaCATACGCAATGAATAAAGCATGGTACGACTCACACCTGACGGTCGCTGGGCAGTGTACGTGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTa                                                                                                                           <  2:17618/146‑1 (MQ=255)                               cATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCtc                                                                                                                           >  8:272440/1‑143 (MQ=255)                                    gCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAtgc                                                                                                                 >  2:4509/1‑149 (MQ=255)                                    gCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAtgc                                                                                                                 <  7:64934/149‑1 (MQ=255)                                      aaTGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                                        <  8:43353/124‑1 (MQ=255)                                           aTAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                                         >  3:287172/1‑134 (MQ=255)                                           aTAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                                         <  4:287172/134‑1 (MQ=255)                                             aaaGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATc                                                                                                         >  8:195124/1‑148 (MQ=255)                                              aaGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTAGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATc                                                                                                         >  8:58893/1‑147 (MQ=255)                                                              acacCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTc                                                                                       <  7:179423/149‑1 (MQ=255)                                                                  cAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGa                                                                                                                                             <  6:9740/91‑1 (MQ=255)                                                                  cAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGa                                                                                                                                             >  5:9740/1‑91 (MQ=255)                                                                  cAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAgc                                                                                   <  6:89148/149‑1 (MQ=255)                                                                    gACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTACAgcgc                                                                                 >  4:261290/1‑149 (MQ=255)                                                                                 cAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCt                                                                    >  7:298297/1‑149 (MQ=255)                                                                                              tgCACTGGCTGGGGCAAACCTTGGTTCAGGCCTGTTTCGGGCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTAGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCa                                                       <  8:8278/149‑1 (MQ=255)                                                                                              tgCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCa                                                       <  8:298297/149‑1 (MQ=255)                                                                                              tgCACTGGCGGGGGCACACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTc                                                        <  6:218257/148‑1 (MQ=255)                                                                                                 aCTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCt                                                    <  5:115882/149‑1 (MQ=255)                                                                                                  ctggctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCtt                                                   >  5:99457/1‑149 (MQ=255)                                                                                                   tggctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                                  >  7:220139/1‑149 (MQ=255)                                                                                                   tggctggGGCAAACCTTGATGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTg                                                  >  8:257065/1‑149 (MQ=255)                                                                                                     gctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTgcgc                                                              <  5:259194/135‑1 (MQ=255)                                                                                                     gctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTgcgc                                                              >  6:259194/1‑135 (MQ=255)                                                                                                         gggCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTc                                            <  6:99457/149‑1 (MQ=255)                                                                                                           gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCCGCTTGGTGTTCtt                                          >  8:267945/1‑149 (MQ=255)                                                                                                           gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCtt                                          >  8:272289/1‑149 (MQ=255)                                                                                                             aaaCCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTtgt                                        <  1:4509/149‑1 (MQ=255)                                                                                                                 cTTCGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCCCACTTTCAGCTTGGTGTTCTAGTgcgc                                    >  5:286018/1‑149 (MQ=255)                                                                                                                         atgCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATg                             <  7:58893/146‑1 (MQ=255)                                                                                                                           gCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGtt                           >  7:34400/1‑148 (MQ=255)                                                                                                                           gCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTc                          <  6:19820/149‑1 (MQ=255)                                                                                                                             cTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAg                        <  7:267945/149‑1 (MQ=255)                                                                                                                             cTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAg                        <  7:272289/149‑1 (MQ=255)                                                                                                                                  aCGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGgtg                                               <  5:324477/121‑1 (MQ=255)                                                                                                                                  aCGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGgtg                                               >  6:324477/1‑121 (MQ=255)                                                                                                                                  aCGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttc                    <  3:301543/148‑1 (MQ=255)                                                                                                                                  aCGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCNGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttct                   >  5:56855/1‑149 (MQ=255)                                                                                                                                    ggCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCttcttc                 <  7:257065/149‑1 (MQ=255)                                                                                                                                    ggCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCttcttc                 <  3:125883/149‑1 (MQ=255)                                                                                                                                      ccc‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAg               >  4:110105/1‑149 (MQ=255)                                                                                                                                       ccttttCGCGGGCTTGCCCGCGAAAGGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAg               >  8:157713/4‑149 (MQ=255)                                                                                                                                        cttttCGCGGGCTTGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGc              >  1:242537/3‑149 (MQ=255)                                                                                                                                              gcgGGCTTGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCcgc        >  6:297594/1‑149 (MQ=255)                                                                                                                                                    aaGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCCGCGCTGCg  >  1:113510/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACGGCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGCCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCTCCCGCGCTGCG  >  NC_002947/2068005‑2068299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑