Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,125,440 G→A 9.1% G271R (GGG→AGG)  PP_1887 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,125,4400GA 9.1% 120.5 / 2.4 44G271R (GGG→AGG) PP_1887hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base A (2/2);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01

AAGCGACAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACTTCATCGGCGGTGGCAGCAGC  >  NC_002947/2125292‑2125586
                                                                                                                                                    |                                                                                                                                                  
aaGCGACAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCg                                                                                                                                                    >  8:258473/1‑149 (MQ=255)
 aGCGACAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCgg                                                                                                                                                   <  7:177578/149‑1 (MQ=255)
     aCAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCa                                                                                                                                                >  6:149062/1‑148 (MQ=255)
      cAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATa                                                                                                                                              <  7:258473/149‑1 (MQ=255)
       aaCCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCAt                                                                                                                                               >  6:246957/1‑147 (MQ=255)
                  tGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAagca                                                                                                                                  <  4:197127/149‑1 (MQ=255)
                   gagaTCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGATGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAagcag                                                                                                                                 >  2:67194/1‑149 (MQ=255)
                            cTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACg                                                                                                                        >  3:266019/1‑149 (MQ=255)
                                    ggCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACaaaa                                                                                                                <  7:173932/149‑1 (MQ=255)
                                      ccAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAAt                                                                                                              <  4:266019/149‑1 (MQ=255)
                                        aaCCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCt                                                                                                            >  5:310596/1‑149 (MQ=255)
                                        aaCCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCt                                                                                                            <  7:87563/149‑1 (MQ=255)
                                                ccGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAggg                                                                                                    <  7:178096/149‑1 (MQ=255)
                                                  gTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCAGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGt                                                                                                   >  2:179688/1‑148 (MQ=255)
                                                  gTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCAGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGt                                                                                                   >  2:184766/1‑148 (MQ=255)
                                                   ttGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGCCGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTa                                                                                                 >  8:139869/1‑149 (MQ=255)
                                                                 aTGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAAt                                                                                    <  2:313509/148‑1 (MQ=255)
                                                                    atgACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGa                                                                                 <  3:109409/146‑1 (MQ=255)
                                                                    aGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGa                                                                                 <  5:149062/148‑1 (MQ=255)
                                                                                ggTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGt                                                                    >  3:32805/1‑149 (MQ=255)
                                                                                 gTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTAACCCGCCATGAAATCGATCCACGCTTTGGt                                                                    >  3:226316/1‑148 (MQ=255)
                                                                                          cGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAAt                                                                                    >  2:237637/1‑123 (MQ=255)
                                                                                          cGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAAt                                                                                    <  1:237637/123‑1 (MQ=255)
                                                                                          cGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGgcggcg                                                           <  5:125924/148‑1 (MQ=255)
                                                                                            tACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGaaa                                                        <  6:310596/149‑1 (MQ=255)
                                                                                             aCGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAg                                                       >  5:9768/1‑149 (MQ=255)
                                                                                               gTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAgtg                                                     >  1:16293/1‑149 (MQ=255)
                                                                                                   aTGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGc                                                 >  6:114610/1‑149 (MQ=255)
                                                                                                         aacaCCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATg                                           <  4:32805/149‑1 (MQ=255)
                                                                                                         aacaCCCTATGGTGGGGT‑GAGCGCGTGTTATACCAGGACGCCGGGCATAAGTTACCAAGCAGCGCAGCACCTGACAAAAAAATGCCCAGGGTTAACCCCCATGAAATAGCTACACGCGTTGGTCAGGCGGCGACAGTTGTGCGGGATg                                           >  8:276267/1‑148 (MQ=255)
                                                                                                          acacCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAgcagcgcagc                                                                                                                           <  1:187824/68‑1 (MQ=255)
                                                                                                          acacCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAgcagcgcagc                                                                                                                           >  2:187824/1‑68 (MQ=255)
                                                                                                              ccTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGaa                                         >  4:84534/1‑146 (MQ=255)
                                                                                                              ccTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGaa                                         <  3:84534/146‑1 (MQ=255)
                                                                                                              ccTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTCGTCAGGCGGCGAAAGTGGTGCGGTATGAATAc                                      >  7:102753/1‑149 (MQ=255)
                                                                                                                    gtggggtggAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGgc                                <  5:114610/149‑1 (MQ=255)
                                                                                                                             aGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCGGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAAc                       <  7:139869/149‑1 (MQ=255)
                                                                                                                              gcgcGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACt                      <  5:246957/149‑1 (MQ=255)
                                                                                                                              gcgcGTGTTCTACCAGGACGCCAGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTTCGGTATGAATACCCCGGCACGCACAACt                      <  1:179688/149‑1 (MQ=255)
                                                                                                                              gcgcGTGTTCTACCAGGACGCCAGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTTCGGTATGAATACCCCGGCACGCACAACt                      <  1:184766/149‑1 (MQ=255)
                                                                                                                                gcgTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACtt                     >  2:171343/1‑148 (MQ=255)
                                                                                                                                        tACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACTTCATcggcgg            >  4:304571/1‑149 (MQ=255)
                                                                                                                                          ccAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAgtggtg                                                  >  7:44347/1‑109 (MQ=255)
                                                                                                                                          ccAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAgtggtg                                                  <  8:44347/109‑1 (MQ=255)
                                                                                                                                                 gCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGACGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACTTCATCGGCGGTGgcagcag   <  1:67194/149‑1 (MQ=255)
                                                                                                                                                  ccGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACTTCATCGGCGGTGgcagcagc  <  6:9768/149‑1 (MQ=255)
                                                                                                                                                    gggCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCAACGCTTTGTTCAGGCGCGGAAAGTGGTAGGGTTTAAAAACCACGGCACGCAAAACTACAACGCGGGTGgcagctc   >  2:254392/1‑144 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAGCGACAACCGGGTCAGTGAGATCATCCTGCCCACGGCCAACCAGGCCCGTTGGCGCTTCACTTATGAGGACGTGTTGGGGTACTTGTGCGTACGTGAATGCACAACACCCTATGGTGGGGTGGAGCGCGTGTTCTACCAGGACGCCGGGCATAAGTTCCCAAGCAGCGCAGCACGTGACAAAAATCTGCCCAGGGTTACCCGCCATGAAATCGATCCACGCTTTGGTCAGGCGGCGAAAGTGGTGCGGTATGAATACCCCGGCACGCACAACTTCATCGGCGGTGGCAGCAGC  >  NC_002947/2125292‑2125586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑