Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,157,393 G→A 7.3% V233I (GTC→ATC)  fabD → malonyl‑CoA‑ACP transacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,157,3930GA 7.3% 158.1 / 2.5 55V233I (GTC→ATC) fabDmalonyl‑CoA‑ACP transacylase
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base A (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

GGCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTGGCCCGGGCAAGGT  >  NC_002947/2157251‑2157538
                                                                                                                                              |                                                                                                                                                 
ggCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACATCa                                                                                                                                                >  2:50622/1‑146 (MQ=255)
ggCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACATCa                                                                                                                                                >  2:45945/1‑146 (MQ=255)
ggCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACATCa                                                                                                                                                <  1:50622/146‑1 (MQ=255)
ggCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACATCa                                                                                                                                                <  1:45945/146‑1 (MQ=255)
  ccAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTg                                                                                                                                             >  4:166739/1‑147 (MQ=255)
          gCCAAGCGCGCCCTGCCACTGGCGGTCACCGTCCCCTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTcgc                                                                                                                                   >  5:58797/1‑149 (MQ=255)
             aaGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTcgccgc                                                                                                                                >  3:170299/1‑149 (MQ=255)
                          cACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGcc                                                                                                                   <  4:30232/149‑1 (MQ=255)
                           aCTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGccc                                                                                                                  <  1:287517/149‑1 (MQ=255)
                               gCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTc                                                                                                                >  8:282217/1‑147 (MQ=255)
                                      gCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGAc                                                                                                       <  1:194212/149‑1 (MQ=255)
                                             gTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTgg                                                                                                <  2:42155/149‑1 (MQ=255)
                                             gTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTgg                                                                                                >  4:121378/1‑149 (MQ=255)
                                                gCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGca                                                                                              <  2:202626/148‑1 (MQ=255)
                                                gCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGca                                                                                              >  1:176337/1‑148 (MQ=255)
                                                  aCTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCGCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAg                                                                                           <  3:26474/149‑1 (MQ=255)
                                                      cgcCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCt                                                                                         <  1:148879/147‑1 (MQ=255)
                                                         ccTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGAc                                                                                                                            >  7:8784/1‑109 (MQ=255)
                                                         ccTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGAc                                                                                                                            <  8:8784/109‑1 (MQ=255)
                                                                  gccagccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTAc                                                                           >  3:110905/1‑149 (MQ=255)
                                                                    cagccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGc                                                                         >  4:264171/1‑149 (MQ=255)
                                                                    cagccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGc                                                                         <  4:170299/149‑1 (MQ=255)
                                                                     agccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCt                                                                        >  3:1788/1‑149 (MQ=255)
                                                                     agccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCt                                                                        >  7:55338/1‑149 (MQ=255)
                                                                     agccGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCt                                                                        >  5:252445/1‑149 (MQ=255)
                                                                      gccgccGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTg                                                                       <  5:88638/149‑1 (MQ=255)
                                                                       ccgccgAGCGCTTTGTCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTgg                                                                      <  5:66307/149‑1 (MQ=255)
                                                                          ccgAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTc                                                                   <  2:176337/149‑1 (MQ=255)
                                                                          ccgAGCGCTTTGCCGAATCCTGCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTc                                                                   <  6:252445/149‑1 (MQ=255)
                                                                            gAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTc                                                                   >  4:80/1‑147 (MQ=255)
                                                                            gAGCGCTATGCCGAATCGGTCAATGCCATCGAATGGAAGGGCCCCCAGAGACCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGa                                                                 <  4:250119/149‑1 (MQ=255)
                                                                              gcgcTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGAGTCCGGTGGTGCAGAACGTCACTGCAGCCGGCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAgt                                                               <  4:306676/149‑1 (MQ=255)
                                                                                gcTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAgtgc                                                             <  7:282217/149‑1 (MQ=255)
                                                                                 cTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCTACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAgtgcg                                                            >  7:230470/1‑149 (MQ=255)
                                                                                  tttGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAgtgcgt                                                           >  8:105765/1‑149 (MQ=255)
                                                                                      ccGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAg                                                       >  7:51184/1‑149 (MQ=255)
                                                                                        gAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAgcc                                                     >  6:213198/1‑147 (MQ=255)
                                                                                         atctggTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGAcc                                                    <  7:184889/145‑1 (MQ=255)
                                                                                          aTCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGAccc                                                   <  3:121378/149‑1 (MQ=255)
                                                                                              gTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGAcc                                                    >  8:184889/1‑144 (MQ=255)
                                                                                                 aaTGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGgccgc                                            <  4:1788/149‑1 (MQ=255)
                                                                                                       aTCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACgg                                      >  5:124172/1‑149 (MQ=255)
                                                                                                       aTCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACgg                                      >  6:279531/1‑149 (MQ=255)
                                                                                                               gAAGGCCCCGCCCCTTCCGGTGGGGCAGAACGTCACTGCAGCCGTCGCCCCCGACCTCGCTGCCCTCAACCACGACCTGCTGGCACAGCTGTACCCGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTc                               <  6:58797/148‑1 (MQ=255)
                                                                                                                     cccGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCg                                  <  1:210683/139‑1 (MQ=255)
                                                                                                                     cccGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCg                                  >  2:210683/1‑139 (MQ=255)
                                                                                                                     cccGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTgg                        >  1:292182/1‑149 (MQ=255)
                                                                                                                      ccGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGt                       >  2:6736/1‑149 (MQ=255)
                                                                                                                           gATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAgt                  <  6:124172/149‑1 (MQ=255)
                                                                                                                           gATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAgt                  <  2:292182/149‑1 (MQ=255)
                                                                                                                                cGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTgg              <  4:110905/148‑1 (MQ=255)
                                                                                                                                cGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGACGAGTGTGGc             >  1:273965/1‑149 (MQ=255)
                                                                                                                                 ggtggtGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTGGcc            >  5:229206/1‑149 (MQ=255)
                                                                                                                                        cAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTGGCCCGGGc       >  3:181388/1‑147 (MQ=255)
                                                                                                                                           aaCGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTGGCCCGGGCAAGGt  >  6:27064/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GGCCAAAGGTGCCAAGCGCGCCCTGCCACTGGCGGTGAGCGTCCCGTCGCACTGCGCCCTGATGAAGCCAGCCGCCGAGCGCTTTGCCGAATCGGTCAATGCCATCGAATGGAAGGCCCCGCAGATTCCGGTGGTGCAGAACGTCACTGCAGCCGTCGCCGCCGACCTCGATGCCCTCAAGCACGACCTGCTGGCACAGCTGTACCAGCCGGTACGCTGGGTCGAGTGCGTGCAGACCCTGGCCGCCAACGGTGCGGTCAACCTGGTCGAGTGTGGCCCGGGCAAGGT  >  NC_002947/2157251‑2157538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑