Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,182,071	G→A	7.1%	intergenic (‑36/‑119)	PP_1935 ← / → PP_5487	Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,182,071	0	G	A	7.1%	162.7 / 2.0	57	intergenic (‑36/‑119)	PP_1935/PP_5487	Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/24);  new base A (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCGAGTGAGTACTTTATT  >  NC_002947/2181924‑2182216                                                                                                                                                    |                                                                                                                                                   cggcAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTAtgc                                                                                                                                                   <  4:3946/149‑1 (MQ=255)     aGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGTGTCGAGGCACGAACAGGCAGTATGCTGcc                                                                                                                                               <  8:264632/149‑1 (MQ=255)       gATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCgg                                                                                                                                             >  3:29580/1‑149 (MQ=255)        aTCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGCATACTGCCTGt                                                                                                                                            <  1:42553/149‑1 (MQ=255)            aaaGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACg                                                                                                                                        >  8:55528/1‑149 (MQ=255)              aGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACTCGGCCTAAGGCATTTTTGAAATTCATCACGCGTACGCGTCCAAAAGGAAGCAGTCAACCTATTGCGTCGAGGCAGGAACAGGCAGTATGCTGCCGGTTACGTc                                                                                                                                      <  1:183470/149‑1 (MQ=255)                     tGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑t                                                                                                                              >  2:230663/1‑149 (MQ=255)                       cGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGACACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑t                                                                                                                              <  1:61497/147‑1 (MQ=255)                          aaGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCAtttt                                                                                                                                 >  5:20077/1‑142 (MQ=255)                          aaGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCAtttt                                                                                                                                 <  6:20077/142‑1 (MQ=255)                           aGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGttt                                                                                                                        <  7:55528/149‑1 (MQ=255)                             gCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTc                                                                                                                      >  7:197314/1‑149 (MQ=255)                                 cGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCt                                                                                                                  >  3:304167/1‑149 (MQ=255)                                  gCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCtt                                                                                                                 >  4:212643/1‑149 (MQ=255)                                      aaCACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTcc                                                                                                              >  6:278526/1‑148 (MQ=255)                                      aaCACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTc                                                                                                               <  3:212643/147‑1 (MQ=255)                                       acacGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCg                                                                                                             <  4:29580/148‑1 (MQ=255)                                        cacGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGa                                                                                                            >  8:292840/1‑148 (MQ=255)                                         acGCAGTTGGTGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGaa                                                                                                           >  8:7380/1‑148 (MQ=255)                                           gCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCAAGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATc                                                                                                        >  7:300739/1‑149 (MQ=255)                                             aGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCTGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCt                                                                                                       >  4:194938/1‑148 (MQ=255)                                             aGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCAAGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCt                                                                                                       >  1:63536/1‑148 (MQ=255)                                              gTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGa                                                                                                     <  8:15047/149‑1 (MQ=255)                                                    ggAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTc                                                                                               >  8:296022/1‑149 (MQ=255)                                                             ggCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTa                                                                                      <  1:110672/149‑1 (MQ=255)                                                               ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCtt                                                                                        >  3:299119/1‑145 (MQ=255)                                                               ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTaga                                                                                    <  7:7380/149‑1 (MQ=255)                                                               ccAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTaga                                                                                    <  5:235591/149‑1 (MQ=255)                                                                  aatgCAATTTTGTAATTCATCAACGGTAAGGGTCCAAAATGTATCATTGAACCTATTGCGTCGCGGCACGAACATGCACTATGCTTCCGGTTAAGTCATTTTCACGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagaga                                                                                  <  1:230663/146‑1 (MQ=255)                                                                  aaGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagagag                                                                                 >  8:203144/1‑149 (MQ=255)                                                                  aaGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTACCGCCTTTagagag                                                                                 >  5:240353/1‑149 (MQ=255)                                                                                    atcaCGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTc                                                                <  7:296022/148‑1 (MQ=255)                                                                                       acgcgtacgcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTg                                                             <  4:287637/148‑1 (MQ=255)                                                                                        cgagtacgcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCACCTTTAGAGAGTCCTCGGGAACGACTTCCTGCa                                                           <  8:300739/146‑1 (MQ=255)                                                                                               gcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagagag                                                                                 <  3:250365/120‑1 (MQ=255)                                                                                               gcgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagagag                                                                                 >  4:250365/1‑120 (MQ=255)                                                                                                cgtCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCTGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCaaaaa                                                   <  3:194938/149‑1 (MQ=255)                                                                                                  tCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACt                                                                  >  1:25740/1‑132 (MQ=255)                                                                                                  tCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACt                                                                  <  2:25740/132‑1 (MQ=255)                                                                                                    cAAAAGGAAGCATTCATCCTATTGCGTCGAGGCACGAACATGCAGTATTCTGCCGGTTACGTCATTTTC‑TTCGTTTTCGCCTTTCCGAAATCTGATTGCTCCCGCCTTTAGATAGTCCTCGGGTACTACTTCCTGCACCCAAAAATTAg                                               <  7:203144/149‑1 (MQ=255)                                                                                                     aaaaGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAg                                               >  5:18515/1‑148 (MQ=255)                                                                                                           aaGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagaga                                                                                  <  7:24288/107‑1 (MQ=255)                                                                                                           aaGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTagaga                                                                                  >  8:24288/1‑107 (MQ=255)                                                                                                           aaGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCa                                        <  4:304167/149‑1 (MQ=255)                                                                                                                   aaCCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAgtgt                                 <  2:75927/148‑1 (MQ=255)                                                                                                                        aTTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAatat                           >  2:165720/1‑149 (MQ=255)                                                                                                                           gCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGa                         <  1:165720/148‑1 (MQ=255)                                                                                                                             gTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATa                       >  8:40938/1‑148 (MQ=255)                                                                                                                                  tgacgtaaCCGGCAGCATACTGCCTGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAAcgc                  >  8:45398/7‑148 (MQ=255)                                                                                                                                   gCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAAcgc                  >  3:75999/1‑147 (MQ=255)                                                                                                                                       gAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCgagtga            >  7:227630/1‑149 (MQ=255)                                                                                                                                       gAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCgagt              >  6:151621/1‑147 (MQ=255)                                                                                                                                        aaCCGGCAGCATACTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAatat                           >  6:204665/1‑133 (MQ=255)                                                                                                                                        aaCCGGCAGCATACTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAatat                           <  5:204665/133‑1 (MQ=255)                                                                                                                                         aCAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAg                                               <  2:231834/112‑1 (MQ=255)                                                                                                                                         aCAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAg                                               >  1:231834/1‑112 (MQ=255)                                                                                                                                             gCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCGAGTGAGTACtt      >  1:292317/1‑149 (MQ=255)                                                                                                                                                 tATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCGAGTGAGTACTttatt  <  7:166639/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CGGCAGGATCAAAAGCTTCCTGCGTAAGGCCTCGCTGAACACGCAGTTGGCGGAATACGCGGCCAAAGGCAATTTTGTAATTCATCACGCGTACGCGTCCAAAAGGAAGCATTCAACCTATTGCGTCGAGGCACGAACAGGCAGTATGCTGCCGGTTACGTCATTTTC‑TGCGTTTTCGCCTTTCCGAAATCTGAGTGCTCCCGCCTTTAGAGAGTCCTCGGGAACGACTTCCTGCACCCAAAAATTAGGATCGCAAGAGTGTAAATATGATAAACGCGAGTGAGTACTTTATT  >  NC_002947/2181924‑2182216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑