Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,192,830	G→T	5.3%	R79L (CGG→CTG)	PP_1938 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,192,830	0	G	T	5.3%	235.0 / 3.5	75	R79L (CGG→CTG)	PP_1938	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/34);  new base T (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

GAGATGTCCGGCGCCTCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTGGATTC  >  NC_002947/2192689‑2192977                                                                                                                                                |                                                                                                                                                    gagaTGTCCGGCGCCTCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCgtgt                                                                                                                                              <  6:131417/149‑1 (MQ=255)   gaTGTCCGGCGCCTTACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGc                                                                                                                                            <  1:5916/149‑1 (MQ=255)    aTGTCCGGCGCCTCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGcc                                                                                                                                           >  1:292113/1‑149 (MQ=255)               cTCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGaaa                                                                                                                                 >  4:150787/1‑148 (MQ=255)                tCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAAc                                                                                                                               >  5:315678/1‑149 (MQ=255)                 cACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGAAGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAAc                                                                                                                               >  8:45044/1‑148 (MQ=255)                  aCGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGAGGGGCGGTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGATGACAATTTTCGGCCGTGTGCATTGAGGGaaacc                                                                                                                               >  1:261063/1‑147 (MQ=255)                    gATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGc                                                                                                                           <  4:151055/149‑1 (MQ=255)                      ttttGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCtt                                                                                                                         <  3:150787/149‑1 (MQ=255)                              gATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAgg                                                                                                                 >  2:285753/1‑149 (MQ=255)                                tCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAACACTCGCTTCAGAGAgggg                                                                                                               <  6:87995/149‑1 (MQ=255)                                 ccGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGt                                                                                                              >  2:46668/1‑149 (MQ=255)                                  cGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGtt                                                                                                             >  2:132906/1‑149 (MQ=255)                                           cGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGa                                                                                                     <  3:19607/148‑1 (MQ=255)                                            ggATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGCCACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATc                                                                                                   >  2:95255/1‑149 (MQ=255)                                                 tCGAAGTCGAGGTCGTAAGCGACATGTCCGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCGTCAGAGAGGGGTTGACCCAGATCCGCTg                                                                                              <  8:249465/149‑1 (MQ=255)                                                   gAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGcc                                                                                            <  2:292113/149‑1 (MQ=255)                                                     aGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGcc                                                                                            >  7:45629/1‑147 (MQ=255)                                                          aGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGccc                                                                                           <  4:313815/143‑1 (MQ=255)                                                          aGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGccc                                                                                           >  3:313815/1‑143 (MQ=255)                                                            ttCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACAcc                                                                                   >  6:216225/1‑149 (MQ=255)                                                                tAATCGAAATGTCTGTAGT‑‑TGGGCTTCGCCGGCTGATAGAACTGATTGTTGACGCGCACGTGATCGCTTAAACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCTAGATCCGCTGCCCAGAACACCTGa                                                                                <  7:45044/148‑1 (MQ=255)                                                                tAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAg                                                                               <  6:162604/149‑1 (MQ=255)                                                                     gccATCTCTGAAGA‑‑GGCGCTGCGCCGGCTCATAGAACTGATGGATGACGCGCACGTGATCGCTTACACTTTTCGTCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGTTTGACCCACAACCCCTGCCCAGAACACCTGAGTTCt                                                                           >  7:204421/3‑148 (MQ=255)                                                                     gACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGCCCCAGATCCGCTGCCCAGAACACCTGAGTTCt                                                                           >  1:113731/1‑148 (MQ=255)                                                                        aTGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAg                                                                       >  6:184422/1‑149 (MQ=255)                                                                          gTCTGAAGA‑‑GGCGCTGGGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGc                                                                      >  8:61049/1‑148 (MQ=255)                                                                            cTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTAt                                                                   >  4:207244/1‑149 (MQ=255)                                                                              gAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAac                                                                                      >  3:6082/1‑128 (MQ=255)                                                                              gAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAac                                                                                      <  4:6082/128‑1 (MQ=255)                                                                               aaGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGATAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTAt                                                                   <  3:270591/146‑1 (MQ=255)                                                                               aaGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTAtc                                                                  <  6:315678/147‑1 (MQ=255)                                                                                 ga‑‑gGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATctct                                                               >  3:252798/1‑148 (MQ=255)                                                                                 ga‑‑gGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTATGAAGCTATctct                                                               >  3:42727/1‑148 (MQ=255)                                                                                  a‑‑gGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGt                                                                              >  2:26579/1‑132 (MQ=255)                                                                                  a‑‑gGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTCACCCAGATCCGCTGCCCAGAACACCTGAGt                                                                              <  1:26579/132‑1 (MQ=255)                                                                                          tGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAg                                                       >  5:40780/1‑149 (MQ=255)                                                                                                ggCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTg                                                 <  1:132906/149‑1 (MQ=255)                                                                                                ggCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTg                                                 <  6:279419/149‑1 (MQ=255)                                                                                                   tGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTgg                                                >  7:219003/1‑147 (MQ=255)                                                                                                   tGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTa                                              <  3:207244/149‑1 (MQ=255)                                                                                                    gATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAg                                             >  2:224958/1‑149 (MQ=255)                                                                                                     aTAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTg                                                                                              >  4:56109/1‑99 (MQ=255)                                                                                                     aTAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTg                                                                                              <  3:56109/99‑1 (MQ=255)                                                                                                        gAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCg                                         <  8:45629/149‑1 (MQ=255)                                                                                                          aCTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGc                                       <  4:252798/149‑1 (MQ=255)                                                                                                            tGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGt                                     >  8:127826/1‑149 (MQ=255)                                                                                                                 gatgACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCa                                >  3:306135/1‑149 (MQ=255)                                                                                                                  atgACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGc                                                      >  4:211178/1‑126 (MQ=255)                                                                                                                  atgACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGc                                                      <  3:211178/126‑1 (MQ=255)                                                                                                                  atgACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCAc                               <  5:216225/149‑1 (MQ=255)                                                                                                                   tgACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCAcc                              >  5:152023/1‑149 (MQ=255)                                                                                                                     aCGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTa                            <  5:266168/149‑1 (MQ=255)                                                                                                                      cgcgCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAg                           <  5:184422/149‑1 (MQ=255)                                                                                                                        atctcGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGtt                         <  7:236603/145‑1 (MQ=255)                                                                                                                           aCGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTAcc                      <  1:285753/149‑1 (MQ=255)                                                                                                                             gTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGtt                         >  8:236603/1‑144 (MQ=255)                                                                                                                             gTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAg                    >  5:330362/1‑149 (MQ=255)                                                                                                                                aTCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGtt                         <  2:326519/141‑1 (MQ=255)                                                                                                                                aTCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGtt                         >  1:326519/1‑141 (MQ=255)                                                                                                                                 tCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTc                                   >  2:253556/1‑130 (MQ=255)                                                                                                                                 tCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTc                                   <  1:253556/130‑1 (MQ=255)                                                                                                                                     tGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAg            >  3:220350/1‑149 (MQ=255)                                                                                                                                       acacTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAacac                                                                                    <  6:95578/75‑1 (MQ=255)                                                                                                                                       acacTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAggg          <  7:61049/149‑1 (MQ=255)                                                                                                                                         acTTTTCTGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCt        >  3:124731/1‑149 (MQ=255)                                                                                                                                         acTTTTCTGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCt        >  3:129590/1‑149 (MQ=255)                                                                                                                                         acTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCt        <  2:113731/149‑1 (MQ=255)                                                                                                                                         acTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCt        >  8:149220/1‑149 (MQ=255)                                                                                                                                          cTTTTCTGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTg       <  4:124731/149‑1 (MQ=255)                                                                                                                                          cTTTTCTGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTg       <  4:129590/149‑1 (MQ=255)                                                                                                                                          cTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTg       <  1:224958/149‑1 (MQ=255)                                                                                                                                           ttttCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCAGCTAGTTACCAGGCCGACAGGGCTgg      >  5:237321/1‑149 (MQ=255)                                                                                                                                           ttttCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTg       >  3:1512/1‑148 (MQ=255)                                                                                                                                              tCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTGGAt    <  6:152023/148‑1 (MQ=255)                                                                                                                                                ggCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTGGAttc  <  5:113246/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GAGATGTCCGGCGCCTCACGATTTTGCCTGATCCGGCTTTTCCGGATGTCGAAGTCGAGTTCGTAAGCGACATGTCTGAAGA‑‑GGCGCTGCGCCGGCTGATAGAACTGATGGATGACGCGCACGTGATCGCTGACACTTTTCGGCCGTGTGCCTTGACGGAAAACTCGCTTCAGAGAGGGGTTGACCCAGATCCGCTGCCCAGAACACCTGAGTTCTGAAGCTATCTCTAACGCCAGCTAGTGGTAGGTCGGCGTTCCCACCTAGTTACCAGGCCGACAGGGCTGGATTC  >  NC_002947/2192689‑2192977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑