Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,215,345 G→A 6.4% intergenic (+159/‑785) PP_1957 → / → PP_5493 Pdr/VanB family oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,215,3450GA 6.4% 184.5 / 2.2 64intergenic (+159/‑785)PP_1957/PP_5493Pdr/VanB family oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base A (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

GGGGCAGGGGGCCAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCCACAGGCTTCCCT  >  NC_002947/2215198‑2215492
                                                                                                                                                   |                                                                                                                                                   
ggggcaggggGCCAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTg                                                                                                                                                     >  4:208913/1‑148 (MQ=255)
   gcaggggGCCAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCAtt                                                                                                                                                 <  1:124108/149‑1 (MQ=255)
        gggCCAGTCACTCTTATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGa                                                                                                                                            <  5:198134/149‑1 (MQ=255)
          gCCAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGc                                                                                                                                          >  4:72392/1‑149 (MQ=255)
           ccAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCg                                                                                                                                         >  1:119764/1‑149 (MQ=255)
                   tctGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGaa                                                                                                                                 >  2:51597/1‑149 (MQ=255)
                   tctGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGaa                                                                                                                                 >  5:280025/1‑149 (MQ=255)
                     tGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAAcc                                                                                                                               >  7:144416/1‑149 (MQ=255)
                         tATCCGCGACCAGAGCCTACTCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGc                                                                                                                            <  6:275819/148‑1 (MQ=255)
                                gACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTACATTGCGGAGCGGCCTAGAACCCGCTTTGCgg                                                                                                                     >  6:145817/1‑148 (MQ=255)
                                  ccAGAGCCAACGCTGTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGGGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAAt                                                                                                                  <  8:254643/149‑1 (MQ=255)
                                   cAGAGCCAACGCTTTGGTTGCGAGGTCTTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAAt                                                                                                                  >  8:248403/1‑148 (MQ=255)
                                   cAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAAt                                                                                                                  >  1:208371/1‑148 (MQ=255)
                                   cAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAAt                                                                                                                  >  2:236185/1‑148 (MQ=255)
                                    agagCCAACGGTTTGCTTGCGAGGTCCTTAAGGATTGGTGCAGGGATGCGCTGTAGTGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCGTCTGCGTTGCTTTGCGGAGCCGCCTAGAACCCTCTGTGCGGaaag                                                                                                                 >  8:122317/1‑146 (MQ=255)
                                        ccAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAAccc                                                                                                            <  1:236185/149‑1 (MQ=255)
                                          aaCGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCTTTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAg                                                                                                          >  1:103458/1‑149 (MQ=255)
                                           aCGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGc                                                                                                         <  3:72392/149‑1 (MQ=255)
                                               tttGGTTGCGAGGTACTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTCCGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGCACCCGCTTTGCGGAATGAACCCAGCGGGt                                                                                                     >  4:97790/1‑149 (MQ=255)
                                                   gTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCgg                                                                                                  <  2:103458/148‑1 (MQ=255)
                                                   gTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCg                                                                                                   >  7:131267/1‑147 (MQ=255)
                                                    ttGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAg                                                                                                <  4:122683/149‑1 (MQ=255)
                                                    ttGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAg                                                                                                >  6:217267/1‑149 (MQ=255)
                                                       cGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAgggg                                                                                             >  5:195519/1‑149 (MQ=255)
                                                        gAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGc                                                                                            <  6:195519/149‑1 (MQ=255)
                                                        gAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGc                                                                                            <  3:188498/149‑1 (MQ=255)
                                                        gAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGc                                                                                            >  1:202832/1‑149 (MQ=255)
                                                           gTCCTTAAGCATTGGTGCACGGATGCTCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCt                                                                                          <  8:260684/148‑1 (MQ=255)
                                                            tCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTg                                                                                        <  5:217267/149‑1 (MQ=255)
                                                               ttAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCGGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCAAGCGGGTCGGAGGGGCCTTGGaa                                                                                     >  1:263901/1‑149 (MQ=255)
                                                               ttAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCTAGCGGGTCGGAGGGGCCTTGGaa                                                                                     >  6:232239/1‑149 (MQ=255)
                                                                 aaGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGaa                                                                                     <  2:119764/147‑1 (MQ=255)
                                                                 aaGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAAt                                                                                    <  8:131267/148‑1 (MQ=255)
                                                                  aGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATc                                                                                   <  2:202832/148‑1 (MQ=255)
                                                                  aGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCa                                                                                  <  7:36857/149‑1 (MQ=255)
                                                                   gCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCaa                                                                                 <  3:208913/149‑1 (MQ=255)
                                                                    cATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAAt                                                                                >  3:299631/1‑149 (MQ=255)
                                                                     aTTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAAtt                                                                               <  6:280025/149‑1 (MQ=255)
                                                                        ggTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGc                                                                             >  5:289342/1‑148 (MQ=255)
                                                                            cACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGt                                                                         <  4:299631/148‑1 (MQ=255)
                                                                            cACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGt                                                                         <  2:208371/148‑1 (MQ=255)
                                                                             aCGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTg                                                                        >  8:15845/1‑148 (MQ=255)
                                                                                     gcTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACACGCTTTGCGCAATGAACCCAGCGGGTCGTAGGGGCCTTGGAATCAATTGCTAGTGCACTGATTc                                                               >  3:264832/1‑149 (MQ=255)
                                                                                         tAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATc                                                                                   >  2:112790/1‑125 (MQ=255)
                                                                                         tAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGt                                                            <  8:242628/148‑1 (MQ=255)
                                                                                               tAGTGAGTTCAGCAATCGCTAACCATTCATGACTACGAGTGTCCTCTGCCTTGCATTGAGGAGCGGACTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGAACTGATTCTGTCGATAgt                                                     >  4:13763/1‑149 (MQ=255)
                                                                                                     gTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAAc                                               <  8:144416/149‑1 (MQ=255)
                                                                                                      ttCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAAc                                               >  5:143905/1‑148 (MQ=255)
                                                                                                          gCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCg                                                                                                        >  3:10912/1‑87 (MQ=255)
                                                                                                          gCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCg                                                                                                        <  4:10912/87‑1 (MQ=255)
                                                                                                             cTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTACATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAAcc                                              <  6:299434/142‑1 (MQ=255)
                                                                                                             cTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTACATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAAcc                                              >  5:299434/1‑142 (MQ=255)
                                                                                                                gCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCtt                                                                                         <  1:159456/96‑1 (MQ=255)
                                                                                                                gCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCtt                                                                                         >  2:159456/1‑96 (MQ=255)
                                                                                                                gCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAaca                                    >  1:154552/1‑149 (MQ=255)
                                                                                                                gCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAaca                                    <  7:248403/149‑1 (MQ=255)
                                                                                                                    aCCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACgg                                 >  7:188393/1‑148 (MQ=255)
                                                                                                                          cATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCa                           <  1:51597/148‑1 (MQ=255)
                                                                                                                              ccTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCAt                      <  8:188393/149‑1 (MQ=255)
                                                                                                                                 aCGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGa                   >  2:245792/1‑149 (MQ=255)
                                                                                                                                  cGAGTGTCCTCTGCCTTTCATTGCGTAGCGGCCTAGAACCGTCTTTGCGTAATGAACCCAGCGGGTCGGATGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCTATAGTGTTAACCACGTCAAACACGGCACTCAGTCATgg                    <  3:13763/147‑1 (MQ=255)
                                                                                                                                          ctgtGCCTTACATTGCGGAGCGGCCTAGAACGCGCTTTGAGGAATGAACCCAGCGGGTCGGAGGTGCCTTGGAATCAATTGCGAGTGCACTGATTCTTTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCcaca           <  5:145817/145‑1 (MQ=255)
                                                                                                                                           tctGCCTTGCATTGCGGAGCGGCCTAGAACCAGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTAATGGACATCCACAgg         >  5:195152/1‑149 (MQ=255)
                                                                                                                                               ccTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCCACAGGCtt      <  6:289342/148‑1 (MQ=255)
                                                                                                                                                  tGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCCACAGGCTTCCCt  >  7:149224/1‑149 (MQ=255)
                                                                                                                                                   gCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCCACAGGCTTCCCt  >  1:264268/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGGCAGGGGGCCAGTCACTCTGATTATCCGCGACCAGAGCCAACGCTTTGGTTGCGAGGTCCTTAAGCATTGGTGCACGGATGCGCTGTAGGGGTAGTGAGTTCAGCACTCGCTAACCATTCATGCCTACGAGTGTCCTCTGCCTTGCATTGCGGAGCGGCCTAGAACCCGCTTTGCGGAATGAACCCAGCGGGTCGGAGGGGCCTTGGAATCAATTGCGAGTGCACTGATTCTGTCGATAGTGTTAACCACGTCAAACACGGCACTCAGTCATGGACATCCACAGGCTTCCCT  >  NC_002947/2215198‑2215492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑