Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,240,340	A→T	13.3%	D190V (GAC→GTC)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,240,340	0	A	T	13.3%	87.0 / 4.2	36	D190V (GAC→GTC)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (19/12);  new base T (3/2);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGC  >  NC_002947/2240196‑2240488                                                                                                                                                 |                                                                                                                                                     ggCAGCCCCTCCAGCACGCTGGCGAGCCGCACGTGAACCCCATGAACGTGCCGACCGAAGGTAACTGCGTGGGGCCGGACATGCTGCGCGGCGATGTCGAGATCCCCTGTGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGc                                                                                                                                                  <  6:55826/148‑1 (MQ=255)      cgccgcCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGcc                                                                                                                                             >  1:73852/1‑149 (MQ=255)        ccgccTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGTACGTCGGCAGGCCgt                                                                                                                                           >  8:1364/1‑148 (MQ=255)               gACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGTACGTCGGCAGGCCGTCGTTCtt                                                                                                                                    <  7:1364/149‑1 (MQ=255)                            cGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTg                                                                                                                       >  3:186979/1‑149 (MQ=255)                                        gCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGaccac                                                                                                           <  2:73852/149‑1 (MQ=255)                                               ggTGCCGAGCGAAGGGATCTGCATGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                                    <  7:244358/149‑1 (MQ=255)                                                     gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                      >  6:123945/1‑125 (MQ=255)                                                     gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                      <  5:123945/125‑1 (MQ=255)                                                      aGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCAc                                                                                             >  3:269236/1‑149 (MQ=255)                                                               aTCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGtgct                                                                                    >  8:177977/1‑149 (MQ=255)                                                                  tGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCg                                                                                 >  8:169963/1‑149 (MQ=255)                                                                        gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                            >  4:316606/1‑148 (MQ=255)                                                                             ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                      >  7:120129/1‑149 (MQ=255)                                                                             ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                      >  6:80083/1‑149 (MQ=255)                                                                              gACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGGGCTGCGTGGGGAAGAGTg                                                                     >  8:295523/1‑149 (MQ=255)                                                                                cctgctgcGCGGCGATGTCGAGAGCCCGTGGGACCGCATGGACATGCAGGGGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGc                                                                   <  3:42268/147‑1 (MQ=255)                                                                                    ctgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGc                                                                >  3:92920/1‑148 (MQ=255)                                                                                           gcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcg                                                        <  7:253642/149‑1 (MQ=255)                                                                                                 tCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCATGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCAACGTGCTGAGTGGGGAAGAGTGGCTGCCATCGGCGACCAAg                                                  >  4:55095/1‑149 (MQ=255)                                                                                                   gagaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                                >  4:183735/1‑149 (MQ=255)                                                                                                         ccGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCa                                           >  3:52051/1‑148 (MQ=255)                                                                                                          cGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa                                         <  6:201458/149‑1 (MQ=255)                                                                                                               gACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTa                                    >  7:197458/1‑149 (MQ=255)                                                                                                                aCCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                   <  4:92920/149‑1 (MQ=255)                                                                                                                  cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                   <  4:268773/147‑1 (MQ=255)                                                                                                                  cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                   >  3:268773/1‑147 (MQ=255)                                                                                                                  cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGa                                 <  8:197458/149‑1 (MQ=255)                                                                                                                       ggACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGAACACCTGATGGGCATCAACCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACt                            >  5:303791/1‑149 (MQ=255)                                                                                                                              cAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCt                      <  3:316606/148‑1 (MQ=255)                                                                                                                                      caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                         <  2:247768/134‑1 (MQ=255)                                                                                                                                      caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                         >  1:247768/4‑137 (MQ=255)                                                                                                                                         gaagtaCGTCGGCAGGCCGTCGTTCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc           >  6:288862/1‑148 (MQ=255)                                                                                                                                         gaagaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc           >  2:44918/1‑148 (MQ=255)                                                                                                                                           agaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAg        <  7:267356/149‑1 (MQ=255)                                                                                                                                            gaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGc       >  6:152625/1‑149 (MQ=255)                                                                                                                                               cgacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgt    >  6:46231/1‑149 (MQ=255)                                                                                                                                                gacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGTGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgtg   <  7:177977/149‑1 (MQ=255)                                                                                                                                                 acgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGc  <  4:52051/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     TGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGC  >  NC_002947/2240196‑2240488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑