| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,795 | G→C | 10.9% | T767T (ACG→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,795 | 0 | G | C | 10.9% | 134.6 / 9.0 | 55 | T767T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (24/25); new base C (4/2); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01 | |||||||||||
CATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196651‑196937 | cATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATCACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCgg < 2:15035/149‑1 (MQ=255) ccGTTTAAGCCGGCATGACCCAGGGCTGCGTCGATTTCGAGACCCCGGCGAATGTCGTGTTCTACAACGGTTCGACCTTCAGCTTCACCATCGAGAACGCCACAGGCTGCAACTTCGAAGAGCTGACCCCGAACCCGACGGCGGCTCa < 2:221723/148‑1 (MQ=11) ttGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GAc > 6:4066/1‑148 (MQ=37) aGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGa > 3:252452/1‑149 (MQ=37) gCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGCCGCCGGCTCA‑GACAACGAt > 6:4702/1‑149 (MQ=255) ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAAGGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATc < 5:4066/149‑1 (MQ=37) ccGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATc < 1:55372/149‑1 (MQ=37) gCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAAAGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAAc < 6:148792/149‑1 (MQ=37) ccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCgg > 5:88945/1‑149 (MQ=34) gggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTc > 4:168948/1‑148 (MQ=34) gCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGcc < 6:158094/149‑1 (MQ=34) gCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGc < 3:168948/149‑1 (MQ=34) gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGa < 7:28353/149‑1 (MQ=32) aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACgg > 1:251932/1‑145 (MQ=32) aaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACgg < 2:251932/145‑1 (MQ=32) cTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCgg > 6:22986/1‑149 (MQ=32) acaacaACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGt > 5:92324/1‑148 (MQ=32) ggTTCGAACGTCAGCCTCACCATCCACAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGTACCCGTCGCCGGCTCATTTCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg < 1:97669/149‑1 (MQ=11) aCCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 4:216474/1‑149 (MQ=32) aCCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt > 8:166125/1‑149 (MQ=32) aCCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt < 6:92324/149‑1 (MQ=32) tCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc > 3:225828/1‑149 (MQ=32) tCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc > 7:297639/1‑149 (MQ=32) tCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGCTCGATGCCACCACCGCGACCCAGACGGCGAGCCCGTAGGTCACCGAAGGCGGCGTGAt > 3:134692/1‑148 (MQ=12) tCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GAACACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCAGTCGGTCACCGAAGGCGGCGTGAt > 8:118672/1‑148 (MQ=17) gCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGc > 3:247579/1‑119 (MQ=32) gCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGc < 4:247579/119‑1 (MQ=32) cGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAcc > 4:158348/1‑148 (MQ=32) gTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTa < 8:88582/149‑1 (MQ=32) agaACGCCACTGGCGGCGACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAcc > 1:47682/1‑149 (MQ=18) gaACGCCACTGGCGGCGACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAccc < 2:47682/149‑1 (MQ=21) gaACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAccc > 2:38014/1‑149 (MQ=34) gaACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAccc > 8:68699/1‑149 (MQ=34) gCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg > 4:60344/1‑124 (MQ=32) gCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg < 3:60344/124‑1 (MQ=32) gcggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATc < 5:22986/147‑1 (MQ=32) gcggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATc < 4:225828/147‑1 (MQ=32) ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAc > 8:6345/1‑134 (MQ=11) ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAc < 7:6345/134‑1 (MQ=11) cAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGccc < 3:158348/149‑1 (MQ=32) aaCTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCa < 7:166125/149‑1 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAg < 8:142949/143‑1 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAg > 7:142949/1‑143 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg < 5:302959/149‑1 (MQ=32) aaCAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACgtga > 7:298118/1‑146 (MQ=17) cccccGAACCCGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCCGACGCCGGTGACAGt > 7:219342/2‑149 (MQ=12) aCCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 3:216474/147‑1 (MQ=32) cccGAACCCGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGCCAGTGa > 3:223410/1‑149 (MQ=9) ccGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCAGCCCAGACGACGGTGACAGTGAc > 3:30156/1‑149 (MQ=17) ccGAACCCGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGTCAGTGAc > 8:207936/1‑149 (MQ=12) cGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 5:4702/149‑1 (MQ=32) cGAACCCGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 1:87789/149‑1 (MQ=17) cGAACCCGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 1:278418/1‑149 (MQ=17) cccGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 6:88945/149‑1 (MQ=11) cccGACCCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 1:99376/149‑1 (MQ=11) | CATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCA‑GACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196651‑196937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |