Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,240,346 T→G 12.2% L192R (CTG→CGG) ‡ gltX → glutamate‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,240,3460TG12.2% 100.4 / 8.4 41L192R (CTG→CGG) ‡gltXglutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (21/15);  new base G (3/2);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

CGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAT  >  NC_002947/2240201‑2240493
                                                                                                                                                 |                                                                                                                                                   
cgccgcCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGcc                                                                                                                                                  >  1:73852/1‑149 (MQ=255)
  ccgccTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGTACGTCGGCAGGCCgt                                                                                                                                                >  8:1364/1‑148 (MQ=255)
         gACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGTACGTCGGCAGGCCGTCGTTCtt                                                                                                                                         <  7:1364/149‑1 (MQ=255)
                      cGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTg                                                                                                                            >  3:186979/1‑149 (MQ=255)
                                  gCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGaccac                                                                                                                <  2:73852/149‑1 (MQ=255)
                                         ggTGCCGAGCGAAGGGATCTGCATGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                                         <  7:244358/149‑1 (MQ=255)
                                               gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                           <  5:123945/125‑1 (MQ=255)
                                               gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                           >  6:123945/1‑125 (MQ=255)
                                                aGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCAc                                                                                                  >  3:269236/1‑149 (MQ=255)
                                                         aTCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGtgct                                                                                         >  8:177977/1‑149 (MQ=255)
                                                            tGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCg                                                                                      >  8:169963/1‑149 (MQ=255)
                                                                  gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                                 >  4:316606/1‑148 (MQ=255)
                                                                       ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                           >  6:80083/1‑149 (MQ=255)
                                                                       ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                           >  7:120129/1‑149 (MQ=255)
                                                                        gACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGGGCTGCGTGGGGAAGAGTg                                                                          >  8:295523/1‑149 (MQ=255)
                                                                          cctgctgcGCGGCGATGTCGAGAGCCCGTGGGACCGCATGGACATGCAGGGGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGc                                                                        <  3:42268/147‑1 (MQ=255)
                                                                              ctgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGc                                                                     >  3:92920/1‑148 (MQ=255)
                                                                                     gcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcg                                                             <  7:253642/149‑1 (MQ=255)
                                                                                           tCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCATGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCAACGTGCTGAGTGGGGAAGAGTGGCTGCCATCGGCGACCAAg                                                       >  4:55095/1‑149 (MQ=255)
                                                                                             gagaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                                     >  4:183735/1‑149 (MQ=255)
                                                                                                   ccGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCa                                                >  3:52051/1‑148 (MQ=255)
                                                                                                    cGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa                                              <  6:201458/149‑1 (MQ=255)
                                                                                                         gACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTa                                         >  7:197458/1‑149 (MQ=255)
                                                                                                          aCCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        <  4:92920/149‑1 (MQ=255)
                                                                                                            cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        <  4:268773/147‑1 (MQ=255)
                                                                                                            cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        >  3:268773/1‑147 (MQ=255)
                                                                                                            cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGa                                      <  8:197458/149‑1 (MQ=255)
                                                                                                                 ggACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGAACACCTGATGGGCATCAACCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACt                                 >  5:303791/1‑149 (MQ=255)
                                                                                                                        cAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCt                           <  3:316606/148‑1 (MQ=255)
                                                                                                                                caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                              <  2:247768/134‑1 (MQ=255)
                                                                                                                                caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                              >  1:247768/4‑137 (MQ=255)
                                                                                                                                   gaagtaCGTCGGCAGGCCGTCGTTCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc                >  6:288862/1‑148 (MQ=255)
                                                                                                                                   gaagaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc                >  2:44918/1‑148 (MQ=255)
                                                                                                                                     agaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAg             <  7:267356/149‑1 (MQ=255)
                                                                                                                                      gaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGc            >  6:152625/1‑149 (MQ=255)
                                                                                                                                         cgacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgt         >  6:46231/1‑149 (MQ=255)
                                                                                                                                          gacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGTGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgtg        <  7:177977/149‑1 (MQ=255)
                                                                                                                                           acgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGc       <  4:52051/149‑1 (MQ=255)
                                                                                                                                            cgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCt      <  3:183735/149‑1 (MQ=255)
                                                                                                                                               ccTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACa   >  6:161103/1‑149 (MQ=255)
                                                                                                                                                cTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAt  <  3:55095/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAT  >  NC_002947/2240201‑2240493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑