Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,240,351	A→T	10.2%	T194S (ACG→TCG)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,240,351	0	A	T	10.2%	108.0 / 3.5	39	T194S (ACG→TCG)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (20/15);  new base T (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

GACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAT  >  NC_002947/2240210‑2240493                                                                                                                                              |                                                                                                                                               gACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGTACGTCGGCAGGCCGTCGTTCtt                                                                                                                                         <  7:1364/149‑1 (MQ=255)              cGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTg                                                                                                                            >  3:186979/1‑149 (MQ=255)                          gCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGaccac                                                                                                                <  2:73852/149‑1 (MQ=255)                                 ggTGCCGAGCGAAGGGATCTGCATGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                                         <  7:244358/149‑1 (MQ=255)                                       gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                           >  6:123945/1‑125 (MQ=255)                                       gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGATGTACTTCCTGGCCAACGTgg                                                                                                                           <  5:123945/125‑1 (MQ=255)                                        aGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCAc                                                                                                  >  3:269236/1‑149 (MQ=255)                                                 aTCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGtgct                                                                                         >  8:177977/1‑149 (MQ=255)                                                    tGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCg                                                                                      >  8:169963/1‑149 (MQ=255)                                                          gtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                                                 >  4:316606/1‑148 (MQ=255)                                                               ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                           >  7:120129/1‑149 (MQ=255)                                                               ggACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                                           >  6:80083/1‑149 (MQ=255)                                                                gACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGGGCTGCGTGGGGAAGAGTg                                                                          >  8:295523/1‑149 (MQ=255)                                                                  cctgctgcGCGGCGATGTCGAGAGCCCGTGGGACCGCATGGACATGCAGGGGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGc                                                                        <  3:42268/147‑1 (MQ=255)                                                                      ctgcGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGc                                                                     >  3:92920/1‑148 (MQ=255)                                                                             gcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcg                                                             <  7:253642/149‑1 (MQ=255)                                                                                   tCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCATGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCAACGTGCTGAGTGGGGAAGAGTGGCTGCCATCGGCGACCAAg                                                       >  4:55095/1‑149 (MQ=255)                                                                                     gagaTCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                                     >  4:183735/1‑149 (MQ=255)                                                                                           ccGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCa                                                >  3:52051/1‑148 (MQ=255)                                                                                            cGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa                                              <  6:201458/149‑1 (MQ=255)                                                                                                 gACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTa                                         >  7:197458/1‑149 (MQ=255)                                                                                                  aCCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        <  4:92920/149‑1 (MQ=255)                                                                                                    cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        <  4:268773/147‑1 (MQ=255)                                                                                                    cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTAc                                        >  3:268773/1‑147 (MQ=255)                                                                                                    cGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGa                                      <  8:197458/149‑1 (MQ=255)                                                                                                         ggACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGAACACCTGATGGGCATCAACCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACt                                 >  5:303791/1‑149 (MQ=255)                                                                                                                cAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCt                           <  3:316606/148‑1 (MQ=255)                                                                                                                        caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                              >  1:247768/4‑137 (MQ=255)                                                                                                                        caggaagtaCGTCGGCAGGCCGTCGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCg                              <  2:247768/134‑1 (MQ=255)                                                                                                                           gaagtaCGTCGGCAGGCCGTCGTTCTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc                >  6:288862/1‑148 (MQ=255)                                                                                                                           gaagaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGccc                >  2:44918/1‑148 (MQ=255)                                                                                                                             agaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAg             <  7:267356/149‑1 (MQ=255)                                                                                                                              gaaCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGc            >  6:152625/1‑149 (MQ=255)                                                                                                                                 cgacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgt         >  6:46231/1‑149 (MQ=255)                                                                                                                                  gacgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGTGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCtgtg        <  7:177977/149‑1 (MQ=255)                                                                                                                                   acgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGc       <  4:52051/149‑1 (MQ=255)                                                                                                                                    cgGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCt      <  3:183735/149‑1 (MQ=255)                                                                                                                                       ccTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACa   >  6:161103/1‑149 (MQ=255)                                                                                                                                        cTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAt  <  3:55095/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               GACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGATGAAGAACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAGCAGCCCAAGCTGTGCTACAT  >  NC_002947/2240210‑2240493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑