Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,255,974 G→T 10.3% intergenic (+34/‑670) asd → / → PP_1990 aspartate‑semialdehyde dehydrogenase/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,255,9740GT10.3% 114.7 / 4.3 39intergenic (+34/‑670)asd/PP_1990aspartate‑semialdehyde dehydrogenase/transposase
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base T (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

CGCAAGCTGAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTG  >  NC_002947/2255827‑2256122
                                                                                                                                                     |                                                                                                                                                    
cgcAAGCTGAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGt                                                                                                                                                     >  6:299495/1‑149 (MQ=255)
  cAAGCTGAACATGGGGGCGCATGACCTGGGCGCGTTCACCGTGGGTGACCAGCTTCTGTTGGGCGCCGCCGAACCGCTGCGCCGCACCTGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGt                                                                                                                                                     <  1:256941/149‑1 (MQ=255)
        gAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGc                                                                                                                                             >  4:292339/1‑149 (MQ=255)
          aCATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGTCACCAGTGCgg                                                                                                                                           <  5:178411/149‑1 (MQ=255)
                     cAGTACCTGGGCGCGTTCACCGTGGGTGGCCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTAt                                                                                                                                <  1:145638/149‑1 (MQ=255)
                      aGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATg                                                                                                                               >  2:218946/1‑149 (MQ=255)
                                    ttCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCa                                                                                                                 <  2:227477/149‑1 (MQ=255)
                                    ttCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCa                                                                                                                 >  6:242311/1‑149 (MQ=255)
                                     tCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCAt                                                                                                                >  2:134895/1‑149 (MQ=255)
                                         cGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTgg                                                                                                            <  5:178522/149‑1 (MQ=255)
                                                gACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCgg                                                                                                     >  4:102759/1‑149 (MQ=255)
                                                            tgGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCg                                                                                         <  2:14652/149‑1 (MQ=255)
                                                              gggCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGa                                                                                       <  1:134895/149‑1 (MQ=255)
                                                                     gccgAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGATAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCa                                                                                >  8:64465/1‑149 (MQ=255)
                                                                                   ccgcA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAAt                                                                  <  3:102759/149‑1 (MQ=255)
                                                                                    cgcA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATg                                                                 <  1:218946/149‑1 (MQ=255)
                                                                                                 gATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCt                                                      >  4:22780/1‑149 (MQ=255)
                                                                                                        ctgCAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGa                                               <  8:132265/149‑1 (MQ=255)
                                                                                                         tgGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGa                                               >  7:35463/1‑148 (MQ=255)
                                                                                                             gCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACagag                                          >  6:26512/1‑149 (MQ=255)
                                                                                                                      gTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccg                                 >  3:4548/1‑149 (MQ=255)
                                                                                                                       ttCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgt                                >  1:2142/1‑149 (MQ=255)
                                                                                                                       ttCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgt                                >  7:182548/1‑149 (MQ=255)
                                                                                                                          aGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgttgc                             >  7:222239/1‑149 (MQ=255)
                                                                                                                              ttCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgcggttgccgtt                         >  7:213170/1‑149 (MQ=255)
                                                                                                                                gcataaaaaaaTACCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACa                                             >  8:193441/7‑127 (MQ=38)
                                                                                                                                gcataaaaaaaTACCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACa                                             <  7:193441/121‑1 (MQ=38)
                                                                                                                                 aCCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGCGCCGTTGCCGTTGCCGttgct                      >  7:162338/1‑149 (MQ=255)
                                                                                                                                    tGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgctttt                   >  7:73779/1‑149 (MQ=255)
                                                                                                                                      aaaaaTACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgctttt                   <  8:213170/147‑1 (MQ=255)
                                                                                                                                         aaTACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGCGCCGTTGCCGTTGCCGttgcttttgcgtt              >  4:71175/1‑149 (MQ=255)
                                                                                                                                           tACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttg            <  3:292339/149‑1 (MQ=255)
                                                                                                                                            aCCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTATTGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           >  4:192661/1‑149 (MQ=255)
                                                                                                                                             ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgct          >  6:34141/1‑149 (MQ=255)
                                                                                                                                             ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           <  5:299495/148‑1 (MQ=255)
                                                                                                                                             ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           <  5:242311/148‑1 (MQ=255)
                                                                                                                                              cGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttg                  >  7:147751/1‑140 (MQ=255)
                                                                                                                                               gaaCTTGTTACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTc        <  8:35463/147‑1 (MQ=255)
                                                                                                                                                cACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCt       <  6:317349/149‑1 (MQ=255)
                                                                                                                                                     gTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTg  >  7:258487/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCAAGCTGAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCA‑‑TGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTG  >  NC_002947/2255827‑2256122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑