Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,255,977 A→C 10.5% intergenic (+37/‑667) asd → / → PP_1990 aspartate‑semialdehyde dehydrogenase/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,255,9770AC10.5% 101.1 / 5.1 38intergenic (+37/‑667)asd/PP_1990aspartate‑semialdehyde dehydrogenase/transposase
Reads supporting (aligned to +/- strand):  ref base A (20/14);  new base C (2/2);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTG  >  NC_002947/2255835‑2256122
                                                                                                                                              |                                                                                                                                                 
gAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGc                                                                                                                                             >  4:292339/1‑149 (MQ=255)
  aCATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGTCACCAGTGCgg                                                                                                                                           <  5:178411/149‑1 (MQ=255)
             cAGTACCTGGGCGCGTTCACCGTGGGTGGCCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTAt                                                                                                                                <  1:145638/149‑1 (MQ=255)
              aGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATg                                                                                                                               >  2:218946/1‑149 (MQ=255)
                            ttCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCa                                                                                                                 >  6:242311/1‑149 (MQ=255)
                            ttCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCa                                                                                                                 <  2:227477/149‑1 (MQ=255)
                             tCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCAt                                                                                                                >  2:134895/1‑149 (MQ=255)
                                 cGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTgg                                                                                                            <  5:178522/149‑1 (MQ=255)
                                        gACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCgg                                                                                                     >  4:102759/1‑149 (MQ=255)
                                                    tgGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCg                                                                                         <  2:14652/149‑1 (MQ=255)
                                                      gggCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGa                                                                                       <  1:134895/149‑1 (MQ=255)
                                                             gccgAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGATAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCa                                                                                >  8:64465/1‑149 (MQ=255)
                                                                           ccgcATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAAt                                                                  <  3:102759/149‑1 (MQ=255)
                                                                            cgcATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATg                                                                 <  1:218946/149‑1 (MQ=255)
                                                                                       gATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCt                                                      >  4:22780/1‑149 (MQ=255)
                                                                                              ctgCAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGa                                               <  8:132265/149‑1 (MQ=255)
                                                                                               tgGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGa                                               >  7:35463/1‑148 (MQ=255)
                                                                                                   gCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACagag                                          >  6:26512/1‑149 (MQ=255)
                                                                                                            gTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccg                                 >  3:4548/1‑149 (MQ=255)
                                                                                                             ttCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgt                                >  1:2142/1‑149 (MQ=255)
                                                                                                             ttCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgt                                >  7:182548/1‑149 (MQ=255)
                                                                                                                aGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgccgttgc                             >  7:222239/1‑149 (MQ=255)
                                                                                                                    ttCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAgccgttgcggttgccgtt                         >  7:213170/1‑149 (MQ=255)
                                                                                                                      gcataaaaaaaTACCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACa                                             >  8:193441/7‑127 (MQ=38)
                                                                                                                      gcataaaaaaaTACCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACa                                             <  7:193441/121‑1 (MQ=38)
                                                                                                                       aCCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGCGCCGTTGCCGTTGCCGttgct                      >  7:162338/1‑149 (MQ=255)
                                                                                                                          tGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgctttt                   >  7:73779/1‑149 (MQ=255)
                                                                                                                            aaaaaTACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgctttt                   <  8:213170/147‑1 (MQ=255)
                                                                                                                               aaTACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGCGCCGTTGCCGTTGCCGttgcttttgcgtt              >  4:71175/1‑149 (MQ=255)
                                                                                                                                 tACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttg            <  3:292339/149‑1 (MQ=255)
                                                                                                                                  aCCGCACTGTCACCAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTATTGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           >  4:192661/1‑149 (MQ=255)
                                                                                                                                   ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgct          >  6:34141/1‑149 (MQ=255)
                                                                                                                                   ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           <  5:242311/148‑1 (MQ=255)
                                                                                                                                   ccGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttgcttttgc           <  5:299495/148‑1 (MQ=255)
                                                                                                                                    cGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGttgcttttg                  >  7:147751/1‑140 (MQ=255)
                                                                                                                                     gaaCTTGTTACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTc        <  8:35463/147‑1 (MQ=255)
                                                                                                                                      cACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCt       <  6:317349/149‑1 (MQ=255)
                                                                                                                                           gTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTg  >  7:258487/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GAACATGGGGTCGCAGTACCTGGGCGCGTTCACCGTGGGTGACCAGCTGCTGTGGGGCGCCGCCGAACCGCTGCGCCGCATGTTGCGGATTCTGCTGGAGCGTTGATCGTTCAGGCTTCACCTGAAAAATACCGCACTGGTGACAGTGCGGTATTTTTTTATGCCTGAAAGTATGCATTTGGGTCGCGGGCCTAATCGCCGGATAATTCATGCCGCTGTTTAATGGCGAAATGTCTGCTCTGACAGAGCCGTTGCCGTTGCCGTTGCTTTTGCTTTTGCTTCTAAGTG  >  NC_002947/2255835‑2256122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑