Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,263,570 T→C 5.2% G121G (GGT→GGC)  trpF → N‑(5'‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,263,5700TC 5.2% 227.2 / 2.4 77G121G (GGT→GGC) trpFN‑(5'‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base T (37/36);  new base C (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CTCGAAGTGGTCCCGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTGGCCAGGCCATTG  >  NC_002947/2263427‑2263718
                                                                                                                                               |                                                                                                                                                    
cTCGAAGTGGTCCCGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCcg                                                                                                                                                 >  6:288623/1‑149 (MQ=255)
  gtagcaggTCCAGCGGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCcgcg                                                                                                                                               >  6:140000/7‑149 (MQ=255)
      gTGGTCCCGCTGGACCTGCTACAGTTTCACGGAGATGAAACCCCGCAGGACTGTGAAGGCTACCAACGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCAGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCAt                                                                                                                                           >  8:201933/1‑149 (MQ=255)
        ggTCCCGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCAttt                                                                                                                                         >  3:85114/1‑149 (MQ=255)
         gTCCCGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCAtttt                                                                                                                                        >  5:287089/1‑149 (MQ=255)
            ccGCTGGACCTGCTACAGTTTCAGGGCGATGAAACCCCGCATGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCt                                                                                                                                     <  2:215655/149‑1 (MQ=255)
            ccGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCt                                                                                                                                     <  1:319911/149‑1 (MQ=255)
             cGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTc                                                                                                                                    <  2:312863/149‑1 (MQ=255)
             cGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTAACACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTc                                                                                                                                    >  7:288013/1‑149 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGCTCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGacac                                                                                                                               <  1:302142/149‑1 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGacac                                                                                                                               >  4:281638/1‑149 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGacac                                                                                                                               >  6:158093/1‑149 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGacac                                                                                                                               <  3:281638/149‑1 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACCCCGCAAGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGac                                                                                                                                 <  7:198343/147‑1 (MQ=255)
                  gACCTGCTACAGTTTCACGGCGATGAAACACCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGacac                                                                                                                               <  1:302145/149‑1 (MQ=255)
                          aCAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTAc                                                                                                                       >  3:197085/1‑149 (MQ=255)
                           cAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTAcc                                                                                                                      <  5:288623/149‑1 (MQ=255)
                                tCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCg                                                                                                                 >  2:18301/1‑149 (MQ=255)
                                tCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGACTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCg                                                                                                                 >  2:289285/1‑149 (MQ=255)
                                    ggCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGc                                                                                                              >  5:89715/1‑148 (MQ=255)
                                     gCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGc                                                                                                              >  4:36884/1‑147 (MQ=255)
                                      cGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAg                                                                                                           >  3:206199/1‑149 (MQ=255)
                                       gATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAgg                                                                                                          <  3:253433/149‑1 (MQ=255)
                                        aTGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAggg                                                                                                         <  4:197085/149‑1 (MQ=255)
                                                  gCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCg                                                                                               <  2:140476/149‑1 (MQ=255)
                                                   cAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGa                                                                                              <  4:134624/149‑1 (MQ=255)
                                                    aGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAg                                                                                             >  6:187920/1‑149 (MQ=255)
                                                    aGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAg                                                                                             <  5:158093/149‑1 (MQ=255)
                                                     ggACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAgg                                                                                            <  6:287089/149‑1 (MQ=255)
                                                           tgAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCATGGGGAACCGGCGAGGCGTTc                                                                                       <  6:89715/148‑1 (MQ=255)
                                                           tgAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGtt                                                                                        <  8:288013/147‑1 (MQ=255)
                                                           tgAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTc                                                                                       >  5:325666/1‑148 (MQ=255)
                                                                 cTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGt                                                                                <  5:187920/149‑1 (MQ=255)
                                                                 cTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGt                                                                                <  2:256204/149‑1 (MQ=255)
                                                                    ccaccGCCCCTGGATGAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCGTTACGCGGGTGCGCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGTCGAGGCGTTCGACTGGTCAt                                                                             <  2:110201/149‑1 (MQ=255)
                                                                          ccccTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTAc                                                                       >  8:126722/1‑149 (MQ=255)
                                                                          ccccTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTAc                                                                       <  4:85114/149‑1 (MQ=255)
                                                                           cccTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTAcc                                                                      >  8:177609/1‑149 (MQ=255)
                                                                             caggATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCgg                                                                    >  8:159257/3‑149 (MQ=255)
                                                                             cTGTATCAAGGCCCTGCGTGTGCGCCCTGGCGATGAGCTGGAGGCGGCGTGTCGTCTTTACGCGGGTGCCGGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCa                                                                              <  4:17383/139‑1 (MQ=255)
                                                                             cTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCa                                                                              >  3:17383/1‑139 (MQ=255)
                                                                               ggATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGgcg                                                                  >  7:78065/1‑149 (MQ=255)
                                                                                    aaGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCt                                                             <  3:36884/149‑1 (MQ=255)
                                                                                    aaGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCt                                                             >  8:11964/1‑149 (MQ=255)
                                                                                     aGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCtt                                                            <  1:18301/149‑1 (MQ=255)
                                                                                      ggCCCTGCGTGTGCTCCCTGGCGATGACCTGGAGGCGGCCTTTCGGCTTTACGCGGGTGCCCGCGGAATTTTTTTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCTGCGAGGCTTTCGACTGGTCATTGGTACCGGCGCGTCTTg                                                           <  7:201933/149‑1 (MQ=255)
                                                                                      ggCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTg                                                           >  5:293607/1‑149 (MQ=255)
                                                                                        cccTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTg                                                           >  4:284140/1‑147 (MQ=255)
                                                                                        cccTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTg                                                           <  3:284140/147‑1 (MQ=255)
                                                                                        cccTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCCCGTCTTgc                                                          >  2:48577/1‑147 (MQ=255)
                                                                                            gCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGc                                                     >  4:242122/1‑149 (MQ=255)
                                                                                            gCGTGTGAGCCCGGGAGATGACCTGGAGGCGGCCTGTAGGCGTTAAGCCGGTGACGGCGGGATTTTGGTCGACGGCGATGTACCGGGCGTGCGCGGGGGAGGCGGGGAGGGGTGGGACGGGTGATTGGTACCGGCGCGGGTTgggcggg                                                     >  2:126360/1‑144 (MQ=255)
                                                                                               tgtgCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGa                                                  >  3:205644/1‑149 (MQ=255)
                                                                                                  gcgcCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGatca                                               >  5:129193/1‑149 (MQ=255)
                                                                                                          gCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTg                                         <  8:158558/147‑1 (MQ=255)
                                                                                                             aTGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggc                                    >  6:29117/1‑149 (MQ=255)
                                                                                                                 ccTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  <  2:296337/147‑1 (MQ=255)
                                                                                                                 ccTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  >  1:296337/1‑147 (MQ=255)
                                                                                                                    ggAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGt                             >  1:115255/1‑149 (MQ=255)
                                                                                                                     gAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTc                            >  4:14179/1‑149 (MQ=255)
                                                                                                                         cggcCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGcc                        <  5:29117/149‑1 (MQ=255)
                                                                                                                          ggcCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGcc                        >  3:207256/1‑148 (MQ=255)
                                                                                                                          ggcCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGcc                        >  5:273354/1‑148 (MQ=255)
                                                                                                                          ggcCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCg                       <  7:126722/149‑1 (MQ=255)
                                                                                                                                tCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAAc                  <  6:293607/148‑1 (MQ=255)
                                                                                                                                tCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCAATCATCCTTGCGGGCGGGCTGTCGGCCGATAAc                  <  8:78065/148‑1 (MQ=255)
                                                                                                                                   gCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGACTGTCGGCCGATAACGTTg              >  6:234039/1‑149 (MQ=255)
                                                                                                                                     atgccgcgGGCACCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  <  6:10153/123‑1 (MQ=255)
                                                                                                                                     atgccgcgGGCACCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  >  5:10153/5‑127 (MQ=255)
                                                                                                                                     atgccgcgGGCACCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  <  2:261932/123‑1 (MQ=255)
                                                                                                                                     atgccgcgGGCACCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTgcgggcgg                                  >  1:261932/5‑127 (MQ=255)
                                                                                                                                       tACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCGAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGAGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTggc            <  7:159257/147‑1 (MQ=255)
                                                                                                                                         cgcgTGTGCCCGCGGCATATTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTggccag         <  5:198304/148‑1 (MQ=255)
                                                                                                                                         cgcgGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTggccag         <  4:206199/148‑1 (MQ=255)
                                                                                                                                          gcgGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTggccaggc       <  4:205644/149‑1 (MQ=255)
                                                                                                                                            gggTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTggccaggcca     >  8:271808/1‑149 (MQ=255)
                                                                                                                                              gTGCCCGCGGCATTTTGCTCGACACCTATGTAGCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTGGCCAGGCCAtt   <  6:325666/149‑1 (MQ=255)
                                                                                                                                               tGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTGGCCAGGCCATTg  <  8:169470/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CTCGAAGTGGTCCCGCTGGACCTGCTACAGTTTCACGGCGATGAAACCCCGCAGGACTGTGAAGGCTACCACCGCCCCTGGATCAAGGCCCTGCGTGTGCGCCCTGGCGATGACCTGGAGGCGGCCTGTCGGCTTTACGCGGGTGCCCGCGGCATTTTGCTCGACACCTATGTACCGGGCGTGCCAGGGGGAACCGGCGAGGCGTTCGACTGGTCATTGGTACCGGCGCGTCTTGGCAAGCCGATCATCCTTGCGGGCGGGCTGTCGGCCGATAACGTTGGCCAGGCCATTG  >  NC_002947/2263427‑2263718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑