Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,313,687 A→T 7.8% I134N (ATT→AAT)  benE‑I ← benzoate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,313,6870AT 7.8% 191.2 / 5.4 64I134N (ATT→AAT) benE‑Ibenzoate transport protein
Reads supporting (aligned to +/- strand):  ref base A (33/26);  new base T (3/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGC  >  NC_002947/2313542‑2313826
                                                                                                                                                 |                                                                                                                                           
ggCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATgc                                                                                                                                          >  7:51904/1‑149 (MQ=255)
    aCCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATgccgc                                                                                                                                       >  5:32487/1‑148 (MQ=255)
    aCCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATgccgc                                                                                                                                       <  6:32487/148‑1 (MQ=255)
     ccACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTTGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGccgccg                                                                                                                                     <  1:320090/149‑1 (MQ=255)
     ccACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGccgccg                                                                                                                                     <  6:58661/149‑1 (MQ=255)
     ccACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGccgccg                                                                                                                                     <  6:216671/149‑1 (MQ=255)
      cACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCATTGCCGCCgg                                                                                                                                    <  3:283806/149‑1 (MQ=255)
        cGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCggg                                                                                                                                   >  7:255864/1‑148 (MQ=255)
        cGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGc                                                                                                                                  <  5:205231/149‑1 (MQ=255)
         ggCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCa                                                                                                                                 >  2:306681/1‑149 (MQ=255)
          gCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCAc                                                                                                                                <  3:305011/149‑1 (MQ=255)
               aCGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTAcgc                                                                                                                           <  8:51904/149‑1 (MQ=255)
                 gggCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTAcgcg                                                                                                                          >  6:151932/1‑148 (MQ=255)
                      gTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAg                                                                                                                    >  3:116879/1‑149 (MQ=255)
                             gTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTc                                                                                                              >  4:93971/1‑148 (MQ=255)
                               ttGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCg                                                                                                             >  5:258020/1‑147 (MQ=255)
                                  aaaGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCa                                                                                                        >  4:74170/1‑149 (MQ=255)
                                     gCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCAccc                                                                                                     >  7:61044/1‑149 (MQ=255)
                                      ccACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCg                                                                                                    >  2:168121/1‑149 (MQ=255)
                                            aCGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGc                                                                                              >  8:243976/1‑149 (MQ=255)
                                                tGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAg                                                                                                          >  2:187349/1‑133 (MQ=255)
                                                tGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAg                                                                                                          <  1:187349/133‑1 (MQ=255)
                                                tGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGccc                                                                                            >  4:183427/1‑147 (MQ=255)
                                                  cAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAg                                                                                         <  6:215442/148‑1 (MQ=255)
                                                  cAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGc                                                                                        >  5:272010/1‑149 (MQ=255)
                                                     tCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGcac                                                                                      >  4:288240/1‑148 (MQ=255)
                                                             ccGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGcc                                                                             >  1:110687/1‑149 (MQ=255)
                                                               ggCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCcgg                                                                           <  7:243976/149‑1 (MQ=255)
                                                                   aGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCggcggc                                                                       <  1:168121/149‑1 (MQ=255)
                                                                   aGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCggcggc                                                                       <  2:110687/149‑1 (MQ=255)
                                                                     gCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCg                                                                     >  3:42649/1‑149 (MQ=255)
                                                                     gCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCg                                                                     <  6:272010/149‑1 (MQ=255)
                                                                      cTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGa                                                                    <  3:74170/149‑1 (MQ=255)
                                                                           gTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGAcagca                                                               <  6:258020/149‑1 (MQ=255)
                                                                                   acAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGa                                                                    >  1:262121/1‑136 (MQ=255)
                                                                                   acAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGa                                                                    <  2:262121/136‑1 (MQ=255)
                                                                                       gCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTa                                                   >  5:323034/1‑149 (MQ=255)
                                                                                       gCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTa                                                   >  1:177073/1‑149 (MQ=255)
                                                                                             aaaGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCt                                             >  6:202641/1‑149 (MQ=255)
                                                                                                     cGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAg                                     <  6:323034/149‑1 (MQ=255)
                                                                                                        tGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGc                                  <  5:151932/149‑1 (MQ=255)
                                                                                                            gAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGc                              >  2:225341/1‑149 (MQ=255)
                                                                                                               cTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTgg                           <  5:202641/149‑1 (MQ=255)
                                                                                                                  cagcagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGc                          >  6:213007/1‑147 (MQ=255)
                                                                                                                  cagcagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGc                          <  3:93971/147‑1 (MQ=255)
                                                                                                                   agcagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAg                       >  1:91463/1‑149 (MQ=255)
                                                                                                                   agcagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAg                       >  4:236960/1‑149 (MQ=255)
                                                                                                                     cagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTgg                           >  1:75335/1‑143 (MQ=255)
                                                                                                                     cagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTgg                           <  2:75335/143‑1 (MQ=255)
                                                                                                                     cagGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAggg                     <  3:288240/149‑1 (MQ=255)
                                                                                                                        gATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGACGCACCCGCTCAGCCGCAGCCCCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCgg                  >  7:98876/1‑149 (MQ=255)
                                                                                                                            ccGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAc              <  1:306681/149‑1 (MQ=255)
                                                                                                                             cGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAc              >  2:183694/1‑148 (MQ=255)
                                                                                                                             cGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAc              >  2:19144/1‑148 (MQ=255)
                                                                                                                              ggCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGAcc             >  4:187289/1‑148 (MQ=255)
                                                                                                                               gCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCaa           <  8:61044/149‑1 (MQ=255)
                                                                                                                                ccAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAg          <  2:177073/149‑1 (MQ=255)
                                                                                                                                 cagcagGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAg          >  3:280636/1‑148 (MQ=255)
                                                                                                                                  agcagGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGaa        >  3:258371/1‑149 (MQ=255)
                                                                                                                                      gcccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAg          >  4:280979/3‑143 (MQ=255)
                                                                                                                                      gcccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAg          <  3:280979/141‑1 (MQ=255)
                                                                                                                                       gCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACgcgg   <  5:213007/149‑1 (MQ=255)
                                                                                                                                        ccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACgcggc  >  6:59492/1‑149 (MQ=255)
                                                                                                                                        ccGGCGGCATTGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACgcggc  >  5:5826/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
GGCGACCACGGCGTAACGGGCGGTGAAGCGTTTGAAAGCCACATACGCTGCAATCAGCAACCCGGCGAGGCTGGGGTCGACCGACAGGCTGGCAAAGGCACCGATGCCGAACTGCAGCAGGATGCCGGCCAGCAGGCCGGCGGCAATGCCGCCGGGCACTACGCGCACCAGCCGCTCGAAGCACCCGCTCAGCCCCAGCACCACGAAGCCGGCGGCCGACAGCAGGTAGGCACCTATGGCCTCGCTGTAGGGCACGCTGGCCAGGGCGGTGACCAAGAACGCGGC  >  NC_002947/2313542‑2313826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑