Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,318,915	C→A	7.4%	P386H (CCT→CAT)	PP_2039 →	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,318,915	0	C	A	7.4%	224.4 / 3.0	74	P386H (CCT→CAT)	PP_2039	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (38/30);  new base A (4/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTGTTGATGC  >  NC_002947/2318769‑2319060                                                                                                                                                   |                                                                                                                                                  cggcaccgcCGTGTATCTGCACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCt                                                                                                                                                  >  2:258853/7‑148 (MQ=255)  ggcaccgcCGTGTATCTGCACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCt                                                                                                                                                  >  5:313091/6‑147 (MQ=255)   ttCGCGGCGTGGAGATACACGGTGGTGCCGGGCTGCGGCGTGGCAACCCGGTGGAGTTGATTTTCCTTGCGTCACGTTTTATGCGCATTCTCACCGGATCCAGCGTGATCCAGCTCAAAACCATCGCCAAGGTCGTGCTCATGCCTGAg                                                                                                                                               <  8:97822/148‑1 (MQ=255)     cgcgCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGc                                                                                                                                              >  1:155478/1‑148 (MQ=255)      gcgcCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCAGGCATGAGCac                                                                                                                                            <  8:273809/149‑2 (MQ=255)       cgcCGTGTATCTGCACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAAt                                                                                                                                           >  5:261574/1‑149 (MQ=255)        gcCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTTCTCCTGAATGAGCAAt                                                                                                                                           >  5:117037/1‑148 (MQ=255)         ccGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGa                                                                                                                                         <  8:288793/149‑1 (MQ=255)         ccGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGa                                                                                                                                         <  8:237366/149‑1 (MQ=255)                     acGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATgccc                                                                                                                             >  5:19928/1‑149 (MQ=255)                       ggcggTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATgcccgg                                                                                                                           >  8:180768/1‑149 (MQ=255)                           gTGCCGGGCTGCTGCGGGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATgcccggcccg                                                                                                                       <  6:313091/149‑1 (MQ=255)                              ccGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCgtgg                                                                                                                    <  8:65425/149‑1 (MQ=255)                                gggCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCgtggtg                                                                                                                  >  6:271746/1‑149 (MQ=255)                                    tgctgcGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCg                                                                                                              >  2:304785/1‑149 (MQ=255)                                      ctgcGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCgg                                                                                                             <  5:271746/148‑1 (MQ=255)                                       tgcGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGt                                                                                                           >  4:252191/1‑149 (MQ=255)                                        gcGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGtt                                                                                                          >  2:48521/1‑149 (MQ=255)                                           tGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTtctc                                                                                                       >  6:60300/1‑149 (MQ=255)                                           tGGAAACCCGGTGGAGTGGATATTCCGTGCGACACGTAAGATGCGCATTCTAAACGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTCAGCAATGACGCGGTATGCCCGGCCCGTCGTGGCCGGGTtctc                                                                                                       >  4:99747/1‑149 (MQ=255)                                              cAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTtctc                                                                                                       >  8:75454/1‑146 (MQ=255)                                                   cGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGAc                                                                                                  >  4:201735/1‑146 (MQ=255)                                                   cGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGAc                                                                                                  >  2:190271/1‑146 (MQ=255)                                                           tggaTATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATaa                                                                                       <  7:180768/149‑1 (MQ=255)                                                                atTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACga                                                                                    >  6:325417/1‑147 (MQ=255)                                                                 ttCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACgagag                                                                                 <  2:41671/149‑1 (MQ=255)                                                                 ttCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACgaga                                                                                  <  2:155478/148‑1 (MQ=255)                                                                     gTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGatca                                                                             <  3:252191/149‑1 (MQ=255)                                                                         gACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAAt                                                                                         >  6:193185/1‑133 (MQ=255)                                                                         gACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAAt                                                                                         <  5:193185/133‑1 (MQ=255)                                                                          acacGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATcatcat                                                                         >  7:121260/1‑148 (MQ=255)                                                                           cacGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATg                                                                        >  6:230267/1‑148 (MQ=255)                                                                            acGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGAc                                                                      <  1:304785/149‑1 (MQ=255)                                                                             cGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACt                                                                     <  8:138153/149‑1 (MQ=255)                                                                              gTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTc                                                                    <  1:190852/149‑1 (MQ=255)                                                                                atgatgCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCa                                                                   >  6:192769/1‑148 (MQ=255)                                                                                 tgatgCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATa                                                                 <  8:121260/149‑1 (MQ=255)                                                                                   atgCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACt                                                               >  5:190354/1‑149 (MQ=255)                                                                                     gcgcATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATCACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACtca                                                             >  7:122413/1‑147 (MQ=255)                                                                                      cgcATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGa                                                             <  8:45994/148‑1 (MQ=255)                                                                                         aTTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACAcc                                                         >  2:253364/1‑149 (MQ=255)                                                                                          ttCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGATATCATCATGACTCATACTGACAccc                                                        >  8:103665/1‑149 (MQ=255)                                                                                             tcACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCg                                                     >  8:186281/1‑149 (MQ=255)                                                                                              cACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGac                                                            >  2:96108/1‑141 (MQ=255)                                                                                              cACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGac                                                            <  1:96108/141‑1 (MQ=255)                                                                                                ccGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGGGGCCGGGTTCTCCAGACAAGAACAATAACGAGATCTCATCATGACTCATACTGACACCCACGc                                                    >  1:77772/1‑147 (MQ=255)                                                                                                  ggAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGt                                                <  7:174010/149‑1 (MQ=255)                                                                                                     agcAGCTAGATCCAGCGGAACTCCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGATCAATAATGAGAGATCATCATGACTCATACTGACACCCCGGCACGTGCa                                             <  8:122413/147‑1 (MQ=255)                                                                                                     aCCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCa                                             <  1:190271/149‑1 (MQ=255)                                                                                                      ccAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAg                                            <  1:253364/149‑1 (MQ=255)                                                                                                          cGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGatca                                                                             <  5:96679/112‑1 (MQ=255)                                                                                                          cGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGatca                                                                             >  6:96679/1‑112 (MQ=255)                                                                                                          cGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCAc                                        >  5:59838/1‑149 (MQ=255)                                                                                                          cGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCAc                                        >  5:27957/1‑149 (MQ=255)                                                                                                              aTCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACgaga                                                                                  <  1:160425/103‑1 (MQ=255)                                                                                                              aTCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACgaga                                                                                  >  2:160425/1‑103 (MQ=255)                                                                                                                   gcgcAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGgat                               <  5:230267/149‑1 (MQ=255)                                                                                                                   gcgcAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGgat                               <  5:60300/149‑1 (MQ=255)                                                                                                                      cAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGgatgat                            >  5:189802/1‑149 (MQ=255)                                                                                                                      cAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACA‑CCCCGCACGTGCAGGCACGGCCTGGATGAtt                           <  1:48521/149‑1 (MQ=255)                                                                                                                            cATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATAc                                                                <  2:5595/107‑1 (MQ=255)                                                                                                                            cATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATAc                                                                >  1:5595/1‑107 (MQ=255)                                                                                                                            cATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCgg                       >  1:5242/1‑148 (MQ=255)                                                                                                                                 ccAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACAccc                                                        <  7:235983/110‑1 (MQ=255)                                                                                                                                 ccAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACAccc                                                        >  8:235983/1‑110 (MQ=255)                                                                                                                                    cgtcattGCTCAGGCATGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTgg              <  2:69717/143‑1 (MQ=255)                                                                                                                                     gggCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACTGCCTGGATGATTTCGGTActgatg               >  3:166107/1‑147 (MQ=255)                                                                                                                                        cGTGCTCATGCCTGAGCAATGACGCGGTATGCACGGCCCGTGGAGGCCGGGTTCACCAGACAACAACAATAACGAGAGATCATCATGACTCATACTGACACCACCGCACGTGCAGGCACGCCCTGGATGATTGCGGTACTGCTGGCGCt          >  4:269854/1‑149 (MQ=255)                                                                                                                                        attGCTCAGGCATGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCt          >  7:262867/3‑149 (MQ=255)                                                                                                                                         ttGCTCAGGCATGAGCAATGCCGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTg         >  6:58129/2‑149 (MQ=255)                                                                                                                                         gTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTg         <  5:192769/149‑1 (MQ=255)                                                                                                                                           gCTCAGGCATGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGCCTCATACTGACACCCACGCACGTGAAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTGtt       >  7:80248/1‑149 (MQ=255)                                                                                                                                              cATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACTTGCAGGCACGGCATGGATGATTGCGGTAATGCTGGCGCTGTTGAt    >  3:314461/1‑149 (MQ=255)                                                                                                                                               aTGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTGTTGa     <  7:103665/147‑1 (MQ=255)                                                                                                                                                ttccTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTGTTGATGc  <  6:261574/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGTGGCAACCCGGTGGAGTGGATATTCCGTGCGACACGTATGATGCGCATTCTCACCGGAACCAGCGAGATCCAGCGCAACACCATCGCCAAGGGCGTGCTCATGCCTGAGCAATGACGCGGTATGCCCGGCCCGTGGTGGCCGGGTTCTCCAGACAAGAACAATAACGAGAGATCATCATGACTCATACTGACACCCCCGCACGTGCAGGCACGGCCTGGATGATTGCGGTACTGCTGGCGCTGTTGATGC  >  NC_002947/2318769‑2319060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑