Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,334,847 C→T 9.3% G537D (GGC→GAC)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,334,8470CT 9.3% 176.9 / 6.9 64G537D (GGC→GAC) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (31/27);  new base T (2/4);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

CAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCG  >  NC_002947/2334709‑2334990
                                                                                                                                           |                                                                                                                                               
cAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGc                                                                                                                                       <  6:273072/149‑1 (MQ=255)
    gcTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                   <  6:309/149‑1 (MQ=255)
     cTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                  <  5:288468/149‑2 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                   <  1:274720/147‑1 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                   >  2:274720/1‑147 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGcc                                                                                                                                 >  5:209017/1‑149 (MQ=255)
        cagaAATGACTCGGCAAAGCGCCTGACAAACTCGGCCATCTGTGGGTATTAGGCCACCAGTGCTGGCTCGAACTCCTGGCGAATCAGGCCCTCGGCCAGTTAACTCACACGCTCATC‑GCCCATGCCCTGGCCGACCCAGGCATGTCCca                                                                                                                               >  6:70546/2‑149 (MQ=255)
               gCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCt                                                                                                                        >  2:176559/1‑149 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                     <  8:90032/147‑1 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                     <  4:275273/147‑1 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                     <  3:90699/147‑1 (MQ=255)
                     gCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTa                                                                                                                  >  6:100640/1‑149 (MQ=255)
                                 accaccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                      >  1:317174/1‑149 (MQ=255)
                                   caccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                    >  3:160279/1‑149 (MQ=255)
                                           ccATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCggg                                                                                            <  5:43769/149‑1 (MQ=255)
                                             aTCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGcc                                                                                          >  4:80499/1‑149 (MQ=255)
                                              tCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCg                                                                                         >  6:164236/1‑149 (MQ=255)
                                                  tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTcc                                                                                     <  1:176559/149‑1 (MQ=255)
                                                   gggTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCca                                                                                    >  1:135515/1‑149 (MQ=255)
                                                     gTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                      >  3:38996/1‑129 (MQ=255)
                                                     gTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                      <  4:38996/129‑1 (MQ=255)
                                                          cGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCg                                                                              <  2:317174/148‑1 (MQ=255)
                                                          cGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCg                                                                              <  4:160279/148‑1 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTGCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCgg                                                                             <  6:209017/148‑1 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                            >  5:158134/1‑149 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                            >  5:318698/1‑149 (MQ=255)
                                                              caccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCt                                                                         <  5:164236/149‑1 (MQ=255)
                                                               accaGTGCGGTCGCGACCTCCTGGAGAATCAGCCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCTTGCCCTGGCCGACGCAGGCATGGCCCATCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCTTTCCACAGGCGGTTCtt                                                                        <  7:66355/149‑1 (MQ=255)
                                                               accaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCtt                                                                        >  7:76494/1‑149 (MQ=255)
                                                                 caGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTtga                                                                      >  7:127530/1‑149 (MQ=255)
                                                                      cGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatc                                                                 <  5:57601/149‑1 (MQ=255)
                                                                       gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCCATGCCCTGGCCGCCCCAGGCATGGCCCAGCAGGATGCCGTACCAGGCACCAATGGCGTGCGGGCCGTTCCACAGGCGGTTGTtgatgagc                                                                 >  1:89687/1‑147 (MQ=255)
                                                                       gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                <  5:85828/149‑1 (MQ=255)
                                                                           gcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                    >  6:70632/1‑109 (MQ=255)
                                                                           gcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                    <  5:70632/109‑1 (MQ=255)
                                                                              aCCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatctg                                                         <  2:135515/149‑1 (MQ=255)
                                                                                      gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                 >  6:16208/1‑149 (MQ=255)
                                                                                      gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                 >  5:106249/1‑149 (MQ=255)
                                                                                        gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                 >  2:90484/1‑147 (MQ=255)
                                                                                        gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                               <  6:106249/149‑1 (MQ=255)
                                                                                          aTCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                             <  3:80499/149‑1 (MQ=255)
                                                                                              ggCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACt                                         >  5:68139/1‑149 (MQ=255)
                                                                                                 gcTCTGCGATTTCACTCACTCGCGCATC‑GCCCATGCCCTGGCCGACGCAGGCATGGCCCAGCATGCTGGCGTACCAGGCCACAATGTAGTGCGGGACGTTCCACAGGCGGTTCTTGATGATATGGATCTGGGCAATGTCTGGCACTGTc                                      <  5:70546/149‑1 (MQ=255)
                                                                                                          gTTCGCTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGGGTACCAGGCCACAATGGCGTGCGGGCCGTGCCAGAGGCGGTGCTTGAGGGGCTGGCTCGGGGGAATGTCTGGCACTGGCTTCACCTgg                             >  2:119339/1‑149 (MQ=255)
                                                                                                              actcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGgcg                           >  4:136136/1‑147 (MQ=255)
                                                                                                              actcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTACACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGtcg                           >  8:146136/1‑147 (MQ=255)
                                                                                                                tcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAgg                       >  2:10859/1‑149 (MQ=255)
                                                                                                                  acCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGTCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGc                      <  1:90484/148‑1 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatct                                                          >  8:52482/1‑110 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatct                                                          <  7:52482/110‑1 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCACTGGCCGACCCAGGCATGGCACAGCAGGCTGGCGTAAAAGGCCACAATGGCGTGCGGGCCGTTCCACAGTCGGTTCTTGATGATCTGGATCTGGGCAATGTGTAGCACTTTCTTAACCTGGCGCAGGCGtt                   >  6:327610/1‑149 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCAc                                 >  2:58783/1‑133 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCAc                                 <  1:58783/133‑1 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCg                 <  2:89687/149‑1 (MQ=255)
                                                                                                                          aTC‑GACCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAg               >  7:210826/1‑147 (MQ=255)
                                                                                                                            c‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAg               >  5:322332/1‑145 (MQ=255)
                                                                                                                             gggccATGCCTGGGTCGGCCAGGGCATGGGCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                             <  7:257483/112‑1 (MQ=37)
                                                                                                                             gggccATGCCTGGGTCGGCCAGGGCATGGGCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                             >  8:257483/4‑115 (MQ=37)
                                                                                                                              gCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGAt          <  3:136136/149‑1 (MQ=255)
                                                                                                                                  aTGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCt      >  7:137418/1‑149 (MQ=255)
                                                                                                                                   tGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg     <  5:100640/149‑1 (MQ=255)
                                                                                                                                   tGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg     <  6:28732/149‑1 (MQ=255)
                                                                                                                                     cccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc   <  6:68139/149‑1 (MQ=255)
                                                                                                                                      ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGGTCCACAGGCGGTTATTGAAGCTCTGGATCTGGGCATTGTCTGGCAATCTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg  >  4:245613/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCG  >  NC_002947/2334709‑2334990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑