Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,334,850 A→G 9.4% V536A (GTC→GCC)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,334,8500AG 9.4% 165.9 / 7.3 65V536A (GTC→GCC) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base G (2/4);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

CAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGC  >  NC_002947/2334709‑2334998
                                                                                                                                              |                                                                                                                                                    
cAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGc                                                                                                                                               <  6:273072/149‑1 (MQ=255)
    gcTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                           <  6:309/149‑1 (MQ=255)
     cTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATggg                                                                                                                                          <  5:288468/149‑2 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                           <  1:274720/147‑1 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCTGGGTCGGCCAGGGCATgg                                                                                                                                           >  2:274720/1‑147 (MQ=255)
      tCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGcc                                                                                                                                         >  5:209017/1‑149 (MQ=255)
        cagaAATGACTCGGCAAAGCGCCTGACAAACTCGGCCATCTGTGGGTATTAGGCCACCAGTGCTGGCTCGAACTCCTGGCGAATCAGGCCCTCGGCCAGTTAACTCACACGCTCATC‑GCCCATGCCCTGGCCGACCCAGGCATGTCCca                                                                                                                                       >  6:70546/2‑149 (MQ=255)
               gCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCt                                                                                                                                >  2:176559/1‑149 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                             <  8:90032/147‑1 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                             <  4:275273/147‑1 (MQ=255)
                    ggCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGc                                                                                                                             <  3:90699/147‑1 (MQ=255)
                     gCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTa                                                                                                                          >  6:100640/1‑149 (MQ=255)
                                 accaccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                              >  1:317174/1‑149 (MQ=255)
                                   caccTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                            >  3:160279/1‑149 (MQ=255)
                                           ccATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCggg                                                                                                    <  5:43769/149‑1 (MQ=255)
                                             aTCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGcc                                                                                                  >  4:80499/1‑149 (MQ=255)
                                              tCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCg                                                                                                 >  6:164236/1‑149 (MQ=255)
                                                  tgGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTcc                                                                                             <  1:176559/149‑1 (MQ=255)
                                                   gggTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCca                                                                                            >  1:135515/1‑149 (MQ=255)
                                                     gTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                              <  4:38996/129‑1 (MQ=255)
                                                     gTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAAt                                                                                                              >  3:38996/1‑129 (MQ=255)
                                                          cGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCg                                                                                      <  4:160279/148‑1 (MQ=255)
                                                          cGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCg                                                                                      <  2:317174/148‑1 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTGCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCgg                                                                                     <  6:209017/148‑1 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                                    >  5:158134/1‑149 (MQ=255)
                                                           ggCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGt                                                                                    >  5:318698/1‑149 (MQ=255)
                                                              caccaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCt                                                                                 <  5:164236/149‑1 (MQ=255)
                                                               accaGTGCGGTCGCGACCTCCTGGAGAATCAGCCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCTTGCCCTGGCCGACGCAGGCATGGCCCATCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCTTTCCACAGGCGGTTCtt                                                                                <  7:66355/149‑1 (MQ=255)
                                                               accaGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCtt                                                                                >  7:76494/1‑149 (MQ=255)
                                                                 caGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTtga                                                                              >  7:127530/1‑149 (MQ=255)
                                                                      cGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatc                                                                         <  5:57601/149‑1 (MQ=255)
                                                                       gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATCGGCCCATGCCCTGGCCGCCCCAGGCATGGCCCAGCAGGATGCCGTACCAGGCACCAATGGCGTGCGGGCCGTTCCACAGGCGGTTGTtgatgagc                                                                         >  1:89687/1‑147 (MQ=255)
                                                                       gggCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatct                                                                        <  5:85828/149‑1 (MQ=255)
                                                                           gcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                            >  6:70632/1‑109 (MQ=255)
                                                                           gcgACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATgg                                                                                                            <  5:70632/109‑1 (MQ=255)
                                                                              aCCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatctg                                                                 <  2:135515/149‑1 (MQ=255)
                                                                                      gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                         >  6:16208/1‑149 (MQ=255)
                                                                                      gCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                         >  5:106249/1‑149 (MQ=255)
                                                                                        gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGt                                                         >  2:90484/1‑147 (MQ=255)
                                                                                        gAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCt                                                       <  6:106249/149‑1 (MQ=255)
                                                                                          aTCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                                     <  3:80499/149‑1 (MQ=255)
                                                                                              ggCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACt                                                 >  5:68139/1‑149 (MQ=255)
                                                                                                 gcTCTGCGATTTCACTCACTCGCGCATC‑GCCCATGCCCTGGCCGACGCAGGCATGGCCCAGCATGCTGGCGTACCAGGCCACAATGTAGTGCGGGACGTTCCACAGGCGGTTCTTGATGATATGGATCTGGGCAATGTCTGGCACTGTc                                              <  5:70546/149‑1 (MQ=255)
                                                                                                          gTTCGCTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGGGTACCAGGCCACAATGGCGTGCGGGCCGTGCCAGAGGCGGTGCTTGAGGGGCTGGCTCGGGGGAATGTCTGGCACTGGCTTCACCTgg                                     >  2:119339/1‑149 (MQ=255)
                                                                                                              actcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGgcg                                   >  4:136136/1‑147 (MQ=255)
                                                                                                              actcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTACACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGtcg                                   >  8:146136/1‑147 (MQ=255)
                                                                                                                tcacCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAgg                               >  2:10859/1‑149 (MQ=255)
                                                                                                                  acCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGTCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGc                              <  1:90484/148‑1 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatct                                                                  >  8:52482/1‑110 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATgatctggatct                                                                  <  7:52482/110‑1 (MQ=255)
                                                                                                                    ccGCGCATC‑GCCCATGCACTGGCCGACCCAGGCATGGCACAGCAGGCTGGCGTAAAAGGCCACAATGGCGTGCGGGCCGTTCCACAGTCGGTTCTTGATGATCTGGATCTGGGCAATGTGTAGCACTTTCTTAACCTGGCGCAGGCGtt                           >  6:327610/1‑149 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCAc                                         <  1:58783/133‑1 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCAc                                         >  2:58783/1‑133 (MQ=255)
                                                                                                                      gcgcATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCg                         <  2:89687/149‑1 (MQ=255)
                                                                                                                          aTC‑GACCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAg                       >  7:210826/1‑147 (MQ=255)
                                                                                                                            c‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAg                       >  5:322332/1‑145 (MQ=255)
                                                                                                                             gggccATGCCTGGGTCGGCCAGGGCATGGGCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                                     <  7:257483/112‑1 (MQ=37)
                                                                                                                             gggccATGCCTGGGTCGGCCAGGGCATGGGCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTgg                                                     >  8:257483/4‑115 (MQ=37)
                                                                                                                              gCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGAt                  <  3:136136/149‑1 (MQ=255)
                                                                                                                                  aTGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCt              >  7:137418/1‑149 (MQ=255)
                                                                                                                                   tGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg             <  6:28732/149‑1 (MQ=255)
                                                                                                                                   tGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTg             <  5:100640/149‑1 (MQ=255)
                                                                                                                                     cccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGcc           <  6:68139/149‑1 (MQ=255)
                                                                                                                                      ccTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGGTCCACAGGCGGTTATTGAAGCTCTGGATCTGGGCATTGTCTGGCAATCTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCcg          >  4:245613/1‑149 (MQ=255)
                                                                                                                                             gACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCgg   <  1:10859/149‑1 (MQ=255)
                                                                                                                                              aCCCAGGCATGGCCCAGCAGGCGGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGc  <  1:119339/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CAGCGCTCGAGAAATGCCTCGGCAAAGCGGCTGACCACCTCGGCCATCTGTGGGTATTCGGCCACCAGTGCGGGCGCGACCTCCTGGCGAATCAGGCGCTCGGCCAGTTCACTCACCCGCGCATC‑GCCCATGCCCTGGCCGACCCAGGCATGGCCCAGCAGGCTGGCGTACCAGGCCACAATGGCGTGCGGGCCGTTCCACAGGCGGTTCTTGATGATCTGGATCTGGGCAATGTCTGGCACTGTCTTCACCTGGCGCAGGCGTTCGAGCAGATCGCTGCCGCGTTCGGC  >  NC_002947/2334709‑2334998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑