Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,333 C→T 8.1% G325G (GGC→GGT)  dctA‑II → C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,3330CT 8.1% 139.9 / 2.2 49G325G (GGC→GGT) dctA‑IIC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base T (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

AACTCGAGAAGCTTGGGTGCAAGAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCGGGGT  >  NC_002947/2340188‑2340476
                                                                                                                                                  |                                                                                                                                                 
aaCTCGAGAAGCTTGGGTGCAAGAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAg                                                                                                                                                <  7:148508/149‑1 (MQ=255)
                   ccaGAAACGCGTGGATCGCCTGGAGCTCCCCACTGCCTACTCCATCAACCAGGACTGAACTGCCATCTATCTGTCGTTGCCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGCCCAGGTGGTCACCATGCTGGCGa                                                                                                                             <  8:116908/147‑1 (MQ=255)
                   cAAGAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGa                                                                                                                             <  3:5972/149‑1 (MQ=255)
                      gAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCAcc                                                                                                                                       >  5:208156/1‑136 (MQ=255)
                      gAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCAcc                                                                                                                                       <  6:208156/136‑1 (MQ=255)
                       aaaGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGTCAGGTGGTCACCATGCTGGCGATCAt                                                                                                                         >  4:238017/1‑149 (MQ=255)
                                       tGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGCTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAg                                                                                                         >  4:812/1‑149 (MQ=255)
                                         gTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAggg                                                                                                       >  4:6655/1‑149 (MQ=255)
                                                ccACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCgg                                                                                                  >  1:13043/1‑147 (MQ=255)
                                                ccACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCgg                                                                                                  >  5:218410/1‑147 (MQ=255)
                                                 cACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAg                                                                                               >  6:168560/1‑149 (MQ=255)
                                                             gTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCg‑‑g                                                                                   >  4:61139/1‑147 (MQ=255)
                                                                   ccTGGACGGTACTGCCATCTATGTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGc                                                                                                 <  7:168387/129‑1 (MQ=255)
                                                                   ccTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGtt                                                                           >  5:137377/1‑149 (MQ=255)
                                                                   ccTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCACGTGGTCACCATGAAGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGc                                                                                                 >  8:168387/1‑129 (MQ=255)
                                                                        aCGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGTAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGtg                                                                      <  4:303558/149‑1 (MQ=255)
                                                                               tGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGTCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGTGGCAGGTGTCACCG‑‑GCAGCGGTTTCGtggccttgg                                                               >  4:144396/1‑149 (MQ=255)
                                                                               tGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑CCAGCGGTTTCGtggccttgg                                                               >  4:209651/1‑149 (MQ=255)
                                                                                        tctGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GCCCTGAGCCTGGGCCAGGTGGGCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGa                                                         <  8:163897/146‑1 (MQ=255)
                                                                                        tctGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGa                                                         <  4:153910/146‑1 (MQ=255)
                                                                                        tctGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGAccc                                                      >  2:265131/1‑149 (MQ=255)
                                                                                           gTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTa                                                   >  6:31126/1‑149 (MQ=255)
                                                                                              gTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCg                                                >  8:60495/1‑149 (MQ=255)
                                                                                                     gCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGTCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCa                                         <  3:238017/149‑1 (MQ=255)
                                                                                                          gtTCATCGCGCAGGCCTGCAACATCGGACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGa                                      >  1:168804/1‑148 (MQ=255)
                                                                                                           ttCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACAt                                   <  6:137377/149‑1 (MQ=255)
                                                                                                                   gcAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTgg                           >  3:255643/1‑149 (MQ=255)
                                                                                                                       gCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCg‑‑g                                                                                   <  8:18745/89‑1 (MQ=255)
                                                                                                                       gCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCg‑‑g                                                                                   >  7:18745/1‑89 (MQ=255)
                                                                                                                         cTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTggccggcc                     >  7:147303/1‑149 (MQ=255)
                                                                                                                          tGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCt                    >  8:294916/1‑149 (MQ=255)
                                                                                                                            cAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTgg                  >  1:320125/1‑149 (MQ=255)
                                                                                                                             aaCATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGt                                               >  7:20069/1‑119 (MQ=255)
                                                                                                                             aaCATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGt                                               <  8:20069/119‑1 (MQ=255)
                                                                                                                               cATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCAc                                        >  1:223802/1‑124 (MQ=255)
                                                                                                                               cATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCAc                                        <  2:223802/124‑1 (MQ=255)
                                                                                                                                aTC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCt              >  6:117515/1‑149 (MQ=255)
                                                                                                                                 ttggACCTTTGCCTGGGCCAGGTGGTAACCATGGGTGCTTTCATGCTGCGGTCCTCCATTGGCGCGGCAGGTGTTACTGACCCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGcc                <  1:265131/146‑1 (MQ=255)
                                                                                                                                 tC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTg             >  4:36996/1‑149 (MQ=255)
                                                                                                                                      ccTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGa         <  5:168560/149‑1 (MQ=255)
                                                                                                                                          aGCCTGGGTCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGTGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATc       <  3:144396/147‑1 (MQ=255)
                                                                                                                                          aGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATc       <  3:812/147‑1 (MQ=255)
                                                                                                                                          aGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCgg     <  7:60495/149‑1 (MQ=255)
                                                                                                                                           gCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCggg    <  5:31126/149‑1 (MQ=255)
                                                                                                                                           gCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCggg    <  3:36996/149‑1 (MQ=255)
                                                                                                                                             cTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCGGGGt  <  2:168804/149‑1 (MQ=255)
                                                                                                                                             cTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCATTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCgggg   >  8:217924/1‑148 (MQ=255)
                                                                                                                                              tGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGtggccttggcct                                                            >  1:183460/1‑90 (MQ=255)
                                                                                                                                              tGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGtggccttggcct                                                            <  2:183460/90‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AACTCGAGAAGCTTGGGTGCAAGAAAGGCGTGGTTGGCCTGGTGCTGCCCACTGGCTACTCGTTCAACCTGGACGGTACTGCCATCTATCTGTCGTTGGCAGCCGTGTTCATCGCGCAGGCCTGCAACATC‑GACCTGAGCCTGGGCCAGGTGGTCACCATGCTGGCGATCATGCTGCTGTCCTCCAAGGGCGCGGCAGGTGTCACCG‑‑GCAGCGGTTTCGTGGCCTTGGCCTCGACCCTTACCGTGATCCACGACATTCCCCTGGCCGGCCTGGCCCTGTTGATCGGGGT  >  NC_002947/2340188‑2340476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑