Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,396,295 A→T 5.1% L1121H (CTT→CAT)  PP_2100 ← two‑component system sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,396,2950AT 5.1% 246.6 / 2.0 78L1121H (CTT→CAT) PP_2100two‑component system sensor protein
Reads supporting (aligned to +/- strand):  ref base A (38/36);  new base T (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TCGCTGGCCTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGG  >  NC_002947/2396148‑2396439
                                                                                                                                                   |                                                                                                                                                
tCGCTGGCCTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAg                                                                                                                                                 <  6:222563/149‑1 (MQ=255)
  gCTGGCCTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGc                                                                                                                                               <  8:45655/149‑1 (MQ=255)
      gCCGCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCTTCAGGCCGACCTGGCCAGCCTGATGGcgc                                                                                                                                             <  6:73412/147‑3 (MQ=255)
       ccTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTgg                                                                                                                                          <  6:261329/149‑1 (MQ=255)
        cTCGAGGGCTTCACAGGCCGCCTTCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCAACTTGTGGGCCTGCTCGGCTATGGCGCGGCTGTCGTTTTGCGGGTGAAGCGCCATCTGGCTGGCCAGGTCGGCCTGAAGGCTCTGGt                                                                                                                                         <  1:192691/149‑1 (MQ=255)
        cTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGt                                                                                                                                         >  3:274631/1‑149 (MQ=255)
        cTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGt                                                                                                                                         >  1:323383/1‑149 (MQ=255)
         tCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTg                                                                                                                                        >  5:141887/1‑149 (MQ=255)
                cTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGtt                                                                                                                                 <  4:64826/149‑1 (MQ=255)
                 ttCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGttt                                                                                                                                >  6:125661/1‑149 (MQ=255)
                 ttCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGttt                                                                                                                                >  2:320311/1‑149 (MQ=255)
                    acaGGCCGCCATCAGGTCCGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCa                                                                                                                             <  4:243189/149‑1 (MQ=255)
                    acaGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGt                                                                                                                                         <  2:51104/137‑1 (MQ=255)
                    acaGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTcc                                                                                                                              <  5:125661/148‑1 (MQ=255)
                      aGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAg                                                                                                                            >  1:153518/1‑148 (MQ=255)
                        gccgccATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCTTCAGGCCGACCTGGCCAGCCTGATgg                                                                                                                                                <  2:308826/126‑1 (MQ=255)
                        gccgccATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCTTCAGGCCGACCTGGCCAGCCTGATgg                                                                                                                                                >  1:308826/1‑126 (MQ=255)
                          cgccATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAgc                                                                                                                       >  1:213178/1‑149 (MQ=255)
                             cATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCt                                                                                                                    <  6:218978/149‑1 (MQ=255)
                             cATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCt                                                                                                                    >  1:190865/1‑149 (MQ=255)
                              aTCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCt                                                                                                                    >  1:41188/1‑148 (MQ=255)
                               tcaggtcaggGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGa                                                                                                                  <  5:118747/149‑1 (MQ=255)
                                       ggggCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAAc                                                                                                          <  1:320311/149‑1 (MQ=255)
                                          gCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCgg                                                                                                       >  8:114650/1‑149 (MQ=255)
                                           cTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCggg                                                                                                      >  5:324527/1‑149 (MQ=255)
                                                      cgcgcgGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGa                                                                                            <  4:224222/148‑1 (MQ=255)
                                                                       cTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCAcggcg                                                                                    >  7:227619/1‑139 (MQ=255)
                                                                       cTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCAcggcg                                                                                    <  8:227619/139‑1 (MQ=255)
                                                                       cTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGt                                                                          >  4:114714/1‑149 (MQ=255)
                                                                       cTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGt                                                                          >  2:85624/1‑149 (MQ=255)
                                                                       cTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGt                                                                          >  6:324329/1‑149 (MQ=255)
                                                                        ttGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGTGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTc                                                                         >  8:225153/1‑149 (MQ=255)
                                                                          gtgGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTc                                                                         <  2:213178/147‑1 (MQ=255)
                                                                               ccTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCGTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCg                                                                           <  8:225055/140‑1 (MQ=255)
                                                                               ccTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCGTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCg                                                                           >  7:225055/1‑140 (MQ=255)
                                                                               ccTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTgg                                                                  <  4:49867/149‑1 (MQ=255)
                                                                               ccTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTgg                                                                  <  2:190865/149‑1 (MQ=255)
                                                                                cTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTgg                                                                  >  3:152081/1‑148 (MQ=255)
                                                                                      ggCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCt                                                           >  1:58580/1‑149 (MQ=255)
                                                                                      ggCAATGGCGCCGCTGTCGTGTGGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAg                                                             <  8:131037/147‑1 (MQ=255)
                                                                                       gCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCtt                                                          <  6:141887/149‑1 (MQ=255)
                                                                                         aaTGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGc                                                        >  8:184741/1‑149 (MQ=255)
                                                                                               gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAg                                                                                                  >  3:117894/1‑101 (MQ=255)
                                                                                               gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAg                                                                                                  <  4:117894/101‑1 (MQ=255)
                                                                                               gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCggg                                                  <  7:114650/149‑1 (MQ=255)
                                                                                               gccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCggg                                                  >  3:142013/1‑149 (MQ=255)
                                                                                                ccgcTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCgggg                                                 <  7:184741/149‑1 (MQ=255)
                                                                                                      tCGTGTTGGGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCGTGAAGGCTCTGGTGCAAGGTTTCCAGGTAGCGCTGACGGTCAACCGGGTCAGGCCCGACCCCGGCGGCCAGGCCGTCCAGCTGGTACAGCTGGCGTCGGGGGCGTTg                                           >  4:6290/1‑149 (MQ=255)
                                                                                                      tCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTg                                           <  5:325055/149‑1 (MQ=255)
                                                                                                      tCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTg                                           >  3:84674/1‑149 (MQ=255)
                                                                                                        gtgtTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGc                                          <  4:142013/148‑1 (MQ=255)
                                                                                                              cGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTggcgg                                    <  4:274631/148‑1 (MQ=255)
                                                                                                                   ccagagcCTTCAGGCCGACCTGGCCAGCCTGATGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCggg                              >  7:285543/6‑149 (MQ=38)
                                                                                                                    aaGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGc                             <  2:58580/149‑1 (MQ=255)
                                                                                                                    aaGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGc                             <  6:205980/149‑1 (MQ=255)
                                                                                                                      gcgcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCg                            >  5:156691/1‑148 (MQ=255)
                                                                                                                      gcgcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCg                            >  4:62529/1‑148 (MQ=255)
                                                                                                                      gcgcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAAACGGGTCAGGCCAGACCACGGCGGACAGGCCGTCCAGCTGGTACAGGTTGGGTGGGGGGGGTTGGTGggggggggggggt                           >  2:128258/1‑146 (MQ=255)
                                                                                                                        gcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACGGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCGGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAAt                         <  3:6290/149‑1 (MQ=255)
                                                                                                                        gcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAAt                         <  6:119116/149‑1 (MQ=255)
                                                                                                                        gcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAAt                         >  3:273975/1‑149 (MQ=255)
                                                                                                                        gcCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAAt                         <  5:324329/149‑1 (MQ=255)
                                                                                                                          cATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCCCGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATgc                       >  5:321987/1‑149 (MQ=255)
                                                                                                                           aTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATgc                       >  5:205444/1‑148 (MQ=255)
                                                                                                                           aTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCAAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTgg                                       >  4:39611/1‑132 (MQ=255)
                                                                                                                           aTCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCAAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTgg                                       <  3:39611/132‑1 (MQ=255)
                                                                                                                              aGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATgccagc                   <  2:153518/149‑1 (MQ=255)
                                                                                                                               ggCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATgccagc                   >  8:301153/1‑148 (MQ=255)
                                                                                                                                  tGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAgg               >  4:302814/1‑149 (MQ=255)
                                                                                                                                      cAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgct           >  2:66955/1‑149 (MQ=255)
                                                                                                                                      cAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgct           >  6:162557/1‑149 (MQ=255)
                                                                                                                                      cAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgct           >  2:198336/1‑149 (MQ=255)
                                                                                                                                      cAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCACCTGGTACAGATTGCGTCGGGGGCGTTGCTGCAGGCGCGGGCGAATGCCAGCCAGGCgct           >  8:40904/1‑149 (MQ=255)
                                                                                                                                        ggTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCgctgg         <  3:302814/149‑1 (MQ=255)
                                                                                                                                            ggCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGa     <  7:40904/149‑1 (MQ=255)
                                                                                                                                              ccTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTATAGCTTGCGTC‑GGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAggg  >  3:98684/1‑149 (MQ=255)
                                                                                                                                              ccTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg   <  6:205444/149‑1 (MQ=255)
                                                                                                                                              ccTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAgg   <  3:62529/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCGCTGGCCTCGAGGGCTTCACAGGCCGCCATCAGGTCAGGGGCTTCCAGCATGCGCGCGGCGCTGAGCACCTTGTGGGCCTGCTCGGCAATGGCGCCGCTGTCGTGTTGCGGGTGAAGCGCCATCAGGCTGGCCAGGTCGGCCTGAAGGCTCTGGTGCAAGGTTTCCAGGAAGCGCTGACGGTCAACCGGGTCAGGCCCGACCACGGCGGCCAGGCCGTCCAGCTGGTACAGCTTGCGTCGGGGGCGTTGCTGGCGGCGCGGGCGAATGCCAGCCAGGCGCTGGCTGAGGG  >  NC_002947/2396148‑2396439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑