Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,407,191 G→A 9.5% D458D (GAC→GAT)  PP_2111 ← aerotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,407,1910GA 9.5% 113.6 / 2.7 42D458D (GAC→GAT) PP_2111aerotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base A (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGTCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGTTGCAGCTTG  >  NC_002947/2407043‑2407329
                                                                                                                                                    |                                                                                                                                           
tGCTGGTCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAACCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATg                                                                                                                                             <  5:24913/149‑1 (MQ=255)
  cTGGTCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGt                                                                                                                                            <  1:165789/148‑1 (MQ=255)
   tGGTCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCg                                                                                                                                          >  3:214377/1‑149 (MQ=255)
      tCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGt                                                                                                                                        >  2:152025/1‑148 (MQ=255)
         gTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTTGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGata                                                                                                                                    >  2:312938/1‑149 (MQ=255)
             agcacTCGGCACTGCTGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTgg                                                                                                                                >  1:180492/5‑149 (MQ=255)
               cGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTg                                                                                                                                 <  1:321607/146‑1 (MQ=255)
                             gAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGAt                                                                                                                >  2:213058/1‑149 (MQ=255)
                                   tgcGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTACTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTAc                                                                                                          >  4:62715/1‑149 (MQ=255)
                                       ccTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAgc                                                                                                      <  4:5561/149‑1 (MQ=255)
                                        cTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCt                                                                                                                   >  4:213076/1‑135 (MQ=255)
                                        cTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCt                                                                                                                   <  3:213076/135‑1 (MQ=255)
                                                      ggTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTg                                                                                        >  6:254550/1‑148 (MQ=255)
                                                       gTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCa                                                                                      >  6:27811/1‑149 (MQ=255)
                                                           gCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTAc                                                                                  <  3:157537/149‑1 (MQ=255)
                                                                          gTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTc                                                                   <  4:214377/149‑1 (MQ=255)
                                                                             cTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCt                                                                 <  5:48212/148‑1 (MQ=255)
                                                                               gATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCtgtg                                                              >  5:308582/1‑149 (MQ=255)
                                                                                aTGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCtgtg                                                              <  5:27811/148‑1 (MQ=255)
                                                                                aTGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCtgtg                                                              <  3:302595/148‑1 (MQ=255)
                                                                                 tGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGcc                                                            <  2:180492/149‑1 (MQ=255)
                                                                                     gCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTgg                                                         <  1:213058/148‑1 (MQ=255)
                                                                                      cGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGc                                                             <  2:196709/143‑1 (MQ=255)
                                                                                      cGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGc                                                             >  1:196709/1‑143 (MQ=255)
                                                                                            aTCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTgg                                                   <  3:62715/147‑1 (MQ=255)
                                                                                            aTCTCGTCAGCCACTGCGGTCTGCTCCTCGCTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCAATACCCGATCGACGCCATCCTGAGCCTGGCGGTGG‑cc                                                >  3:85844/1‑149 (MQ=255)
                                                                                               tcGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGt                                             >  5:315190/1‑149 (MQ=255)
                                                                                                    aGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCT‑GGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTAGCGGTGG‑CCGGTTTCCAt                                       >  4:246089/1‑149 (MQ=255)
                                                                                                     gCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCAt                                       <  1:152025/149‑1 (MQ=255)
                                                                                                             ggTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCa                               <  6:315190/149‑1 (MQ=255)
                                                                                                                     cctcGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTg                       >  2:209792/1‑149 (MQ=255)
                                                                                                                       tcGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATACCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCt                     >  2:71246/1‑149 (MQ=255)
                                                                                                                               agcagcaATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCt             >  4:36535/1‑149 (MQ=255)
                                                                                                                               agcagcaATCTGGGTGGCCATATCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCt                     >  5:297405/1‑141 (MQ=255)
                                                                                                                               agcagcaATCTGGGTGGCCATATCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCt                     >  5:302246/1‑141 (MQ=255)
                                                                                                                               agcagcaATCTGGGTGGCCATATCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCt                     <  6:297405/141‑1 (MQ=255)
                                                                                                                               agcagcaATCTGGGTGGCCATATCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCt                     <  6:302246/141‑1 (MQ=255)
                                                                                                                                  agcaATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGtt          >  3:161894/1‑149 (MQ=255)
                                                                                                                                  agcaATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGtt          <  1:312938/149‑1 (MQ=255)
                                                                                                                                     aaTCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTttgttg         >  5:139443/1‑147 (MQ=255)
                                                                                                                                     aaTCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGTTg         >  5:48031/1‑147 (MQ=255)
                                                                                                                                           ggTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGTTGCAGCTTg  <  1:209792/148‑1 (MQ=255)
                                                                                                                                           ggTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATACCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGGACCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGTTGCAGCtt   <  1:71246/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
TGCTGGTCAGTTCTTCGCTCAGCAGTGCCGAGTGCTGCGCCTGTTCGGCGGTCTGGTCGGCCAGTTCGGCAATGGTGCTGATGTTGCGGCTGATCTCGTCAGCCACTGCGGTCTGCTCCTCGGTTGCAGCAGCAATCTGGGTGGCCATGTCGGTGATATTGGCCACCGCTTCGCTGATGCCTACCAGCGCCTCGTCGGCCTGCATTACCCGATCGACGCCTTCCTGTGCCTGGCGGTGG‑CCGGTTTCCATGGTCAGCACTGCGTTGCTGGCGGTCTGTTGCAGCTTG  >  NC_002947/2407043‑2407329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑