Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,418,820 T→C 11.7% intergenic (+91/‑30) PP_2119 → / → ctpH ABC transporter permease/ATP‑binding protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,418,8200TC11.7% 127.7 / 7.6 51intergenic (+91/‑30)PP_2119/ctpHABC transporter permease/ATP‑binding protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base C (3/3);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

ATTGAGGACAGCCCGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGATG  >  NC_002947/2418673‑2418963
                                                                                                                                                   |                                                                                                                                               
attgaGGACAGCCCGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGt                                                                                                                                                 >  1:106971/1‑148 (MQ=255)
           cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttt                                                                                                                                     >  4:57760/1‑149 (MQ=255)
           cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttt                                                                                                                                     >  7:225523/1‑149 (MQ=255)
           cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                     <  2:108398/149‑4 (MQ=255)
            ccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCtttt                                                                                                                                    >  6:71657/1‑149 (MQ=255)
             cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttttt                                                                                                                                   >  3:285785/1‑149 (MQ=255)
             cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                     <  3:70355/147‑4 (MQ=255)
             cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                     >  4:70355/1‑144 (MQ=255)
              gTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCtttttt                                                                                                                                  <  7:166145/149‑1 (MQ=255)
                  tATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTg                                                                                                                               <  6:65345/148‑1 (MQ=255)
                     gcgcGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAg                                                                                                                                         <  4:6114/135‑1 (MQ=255)
                     gcgcGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAg                                                                                                                                         >  3:6114/1‑135 (MQ=255)
                        cgGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGc                                                                                                                         >  6:154461/1‑148 (MQ=255)
                           cTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGt                                                                                                                     <  4:44020/149‑1 (MQ=255)
                                 gcgcTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAg                                                                                                               <  5:71657/149‑1 (MQ=255)
                                     tGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                           >  8:194538/1‑149 (MQ=255)
                                     tGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                           >  4:190170/1‑149 (MQ=255)
                                       cAATTCACCACGTGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                           <  6:208931/147‑1 (MQ=255)
                                       cAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                         <  5:23003/149‑1 (MQ=255)
                                             accacGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCg                                                                                                    <  5:154461/148‑1 (MQ=255)
                                                acGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCt                                                                                                <  3:57760/149‑1 (MQ=255)
                                                  gggCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAACATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCa                                                                                              <  2:274220/149‑1 (MQ=255)
                                                    gCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGc                                                                                            <  7:93053/149‑1 (MQ=255)
                                                    gCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGc                                                                                            <  7:194538/149‑1 (MQ=255)
                                                        gCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                         <  6:209909/132‑1 (MQ=255)
                                                        gCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                         >  5:209909/1‑132 (MQ=255)
                                                                     ttGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGACATTGCGTGATGGCAGCTTTTTTTTGGCATGCCAGTAATGAGGCTGTACAGCGGCCTAAGCCTACTCCCTCTGGTTcc                                                                           >  3:34412/1‑149 (MQ=255)
                                                                        tAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTcc                                                                           >  5:68781/1‑146 (MQ=255)
                                                                                   tagtaATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGTGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGt                                                             >  3:246020/1‑149 (MQ=255)
                                                                                     gtaATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGtt                                                            <  8:225523/148‑1 (MQ=255)
                                                                                               gagaAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATccc                                                 >  8:76838/1‑149 (MQ=255)
                                                                                                agaagaTTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCt                                                <  4:285785/149‑1 (MQ=255)
                                                                                                            tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCTGAGACCCTCGATGTTGCGt                                                        >  4:44944/1‑129 (MQ=255)
                                                                                                            tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCTGAGACCCTCGATGTTGCGt                                                        <  3:44944/129‑1 (MQ=255)
                                                                                                            tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                    >  1:118570/1‑149 (MQ=255)
                                                                                                            tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                    >  1:240710/1‑149 (MQ=255)
                                                                                                            tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                    >  6:141797/1‑149 (MQ=255)
                                                                                                              aaCTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATtc                                  >  3:135485/1‑149 (MQ=255)
                                                                                                                 tGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTg                                                           >  6:24824/1‑121 (MQ=255)
                                                                                                                 tGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTg                                                           <  5:24824/121‑1 (MQ=255)
                                                                                                                            tGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggc                    <  3:190170/149‑1 (MQ=255)
                                                                                                                             gCTCGACCTGGCTGCCAGTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcg                   >  4:284262/1‑149 (MQ=255)
                                                                                                                              cTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                  >  3:96489/1‑149 (MQ=255)
                                                                                                                               tCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                  >  3:292707/1‑148 (MQ=255)
                                                                                                                                aaaaaaagCTGCCATCACGCAATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                  >  5:280024/8‑147 (MQ=255)
                                                                                                                                  aCCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGATACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCCCTCGCTGCTCGGCGGCAgt              <  7:76838/149‑1 (MQ=255)
                                                                                                                                            cATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGTGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGa    <  4:246020/149‑1 (MQ=255)
                                                                                                                                             aTTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAt   >  4:197218/1‑149 (MQ=255)
                                                                                                                                             aTTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAt   >  1:143569/1‑149 (MQ=255)
                                                                                                                                              ttGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAtg  >  5:129896/1‑149 (MQ=255)
                                                                                                                                               tGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGc      <  3:70717/144‑1 (MQ=255)
                                                                                                                                               tGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGc      >  4:70717/1‑144 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ATTGAGGACAGCCCGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGATG  >  NC_002947/2418673‑2418963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑