Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,418,821 G→A 11.7% intergenic (+92/‑29) PP_2119 → / → ctpH ABC transporter permease/ATP‑binding protein/methyl‑accepting chemotaxis protein CtpH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,418,8210GA11.7% 131.0 / 7.2 51intergenic (+92/‑29)PP_2119/ctpHABC transporter permease/ATP‑binding protein/methyl‑accepting chemotaxis protein CtpH
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base A (3/3);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

CCCGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGATGCTGCT  >  NC_002947/2418684‑2418968
                                                                                                                                         |                                                                                                                                                   
cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttt                                                                                                                                          >  4:57760/1‑149 (MQ=255)
cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttt                                                                                                                                          >  7:225523/1‑149 (MQ=255)
cccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                          <  2:108398/149‑4 (MQ=255)
 ccGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCtttt                                                                                                                                         >  6:71657/1‑149 (MQ=255)
  cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCttttt                                                                                                                                        >  3:285785/1‑149 (MQ=255)
  cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                          >  4:70355/1‑144 (MQ=255)
  cGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAGccag                                                                                                                                          <  3:70355/147‑4 (MQ=255)
   gTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCtttttt                                                                                                                                       <  7:166145/149‑1 (MQ=255)
       tATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTg                                                                                                                                    <  6:65345/148‑1 (MQ=255)
          gcgcGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAg                                                                                                                                              <  4:6114/135‑1 (MQ=255)
          gcgcGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATCACGCAATGGCAg                                                                                                                                              >  3:6114/1‑135 (MQ=255)
             cgGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGc                                                                                                                              >  6:154461/1‑148 (MQ=255)
                cTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGt                                                                                                                          <  4:44020/149‑1 (MQ=255)
                      gcgcTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAg                                                                                                                    <  5:71657/149‑1 (MQ=255)
                          tGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                                >  8:194538/1‑149 (MQ=255)
                          tGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                                >  4:190170/1‑149 (MQ=255)
                            cAATTCACCACGTGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTg                                                                                                                <  6:208931/147‑1 (MQ=255)
                            cAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                              <  5:23003/149‑1 (MQ=255)
                                  accacGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCg                                                                                                         <  5:154461/148‑1 (MQ=255)
                                     acGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCt                                                                                                     <  3:57760/149‑1 (MQ=255)
                                       gggCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAACATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCa                                                                                                   <  2:274220/149‑1 (MQ=255)
                                         gCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGc                                                                                                 <  7:194538/149‑1 (MQ=255)
                                         gCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGc                                                                                                 <  7:93053/149‑1 (MQ=255)
                                             gCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                              <  6:209909/132‑1 (MQ=255)
                                             gCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTa                                                                                                              >  5:209909/1‑132 (MQ=255)
                                                          ttGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGACATTGCGTGATGGCAGCTTTTTTTTGGCATGCCAGTAATGAGGCTGTACAGCGGCCTAAGCCTACTCCCTCTGGTTcc                                                                                >  3:34412/1‑149 (MQ=255)
                                                             tAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTcc                                                                                >  5:68781/1‑146 (MQ=255)
                                                                        tagtaATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGTGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGt                                                                  >  3:246020/1‑149 (MQ=255)
                                                                          gtaATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGtt                                                                 <  8:225523/148‑1 (MQ=255)
                                                                                    gagaAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATccc                                                      >  8:76838/1‑149 (MQ=255)
                                                                                     agaagaTTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCt                                                     <  4:285785/149‑1 (MQ=255)
                                                                                                 tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCTGAGACCCTCGATGTTGCGt                                                             >  4:44944/1‑129 (MQ=255)
                                                                                                 tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCTGAGACCCTCGATGTTGCGt                                                             <  3:44944/129‑1 (MQ=255)
                                                                                                 tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                         >  1:118570/1‑149 (MQ=255)
                                                                                                 tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                         >  1:240710/1‑149 (MQ=255)
                                                                                                 tCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAAt                                         >  6:141797/1‑149 (MQ=255)
                                                                                                   aaCTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATtc                                       >  3:135485/1‑149 (MQ=255)
                                                                                                      tGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTg                                                                >  6:24824/1‑121 (MQ=255)
                                                                                                      tGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTg                                                                <  5:24824/121‑1 (MQ=255)
                                                                                                                 tGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggc                         <  3:190170/149‑1 (MQ=255)
                                                                                                                  gCTCGACCTGGCTGCCAGTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcg                        >  4:284262/1‑149 (MQ=255)
                                                                                                                   cTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                       >  3:96489/1‑149 (MQ=255)
                                                                                                                    tCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                       >  3:292707/1‑148 (MQ=255)
                                                                                                                     aaaaaaagCTGCCATCACGCAATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTcggcgg                       >  5:280024/8‑147 (MQ=255)
                                                                                                                       aCCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGATACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCCCTCGCTGCTCGGCGGCAgt                   <  7:76838/149‑1 (MQ=255)
                                                                                                                                 cATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGTGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGa         <  4:246020/149‑1 (MQ=255)
                                                                                                                                  aTTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAt        >  4:197218/1‑149 (MQ=255)
                                                                                                                                  aTTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAt        >  1:143569/1‑149 (MQ=255)
                                                                                                                                   ttGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAtg       >  5:129896/1‑149 (MQ=255)
                                                                                                                                    tGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGc           <  3:70717/144‑1 (MQ=255)
                                                                                                                                    tGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGc           >  4:70717/1‑144 (MQ=255)
                                                                                                                                         gATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCGAACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGATgctgct  >  1:84736/1‑148 (MQ=255)
                                                                                                                                         gATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAtgc      >  8:11057/1‑144 (MQ=255)
                                                                                                                                         gATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGAtgc      <  7:11057/144‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CCCGTTGTATGCGCGGCTGGCAGCGCTGCAATTCACCACGGGCTAGCGTGTCGTGGCCTTGTAACATTTCTGTAGTAATTGCCTGAGAAGATTTGCCTCAACTGTTGCGTAAGTGCTCGACCTGGCTGCCATTGCGTGATGGCAGCTTTTTTTTGGCCTGCCAGTAATGAGGCTGTACAGCGGCCTCAGCCTACTCCCTCTGGTTCCGAGACCCTCGATGTTGCGTAAATCCCTCAGAGTGCAAATTCTCTCGCTGCTCGGCGGCAGTGTGCTGGCGATGCTGCT  >  NC_002947/2418684‑2418968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑