Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,443,500 T→A 10.9% F6I (TTT→ATT) ‡ PP_2140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,443,5000TA10.9% 145.6 / 8.2 55F6I (TTT→ATT) ‡PP_2140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base A (3/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

CGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAC  >  NC_002947/2443359‑2443644
                                                                                                                                             |                                                                                                                                                
cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                            <  1:315570/148‑1 (MQ=255)
  cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                         <  4:37676/149‑1 (MQ=255)
  cTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                         <  3:69123/149‑1 (MQ=255)
     cAGCCGGAATACAAAGGATCAGTATGTGCTGTCCGAGGTGGATTGCAAGCGTTCCGGTTGTAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                      <  6:36654/149‑1 (MQ=255)
     cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                      >  5:36654/1‑149 (MQ=255)
     cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCa                                                                                                                                       <  4:77428/148‑1 (MQ=255)
     cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGcccc                                                                                                                                        <  4:301026/147‑1 (MQ=255)
      aGCCGCAAGCCCAAGGAGCAGTATGGGCAGTCCGATGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGAAAAGCGCTTATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAAt                                                                                                                                     <  7:121353/149‑1 (MQ=255)
        ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  8:230452/1‑149 (MQ=255)
        ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaatc                                                                                                                                   <  1:196650/149‑4 (MQ=255)
         cGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGAAAGAAGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGCTTGAAGATAAGCGCTGATCGATTGGTGATGTTATTTGCGGCCGCTTTTCGGCCCCaataat                                                                                                                                  >  7:214182/1‑149 (MQ=255)
          gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  6:165319/1‑147 (MQ=255)
          gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   <  5:165319/147‑1 (MQ=255)
           cAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                                <  7:215301/149‑1 (MQ=255)
            aaGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTa                                                                                                                               >  4:184586/1‑149 (MQ=255)
                   aGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                        <  1:246246/149‑1 (MQ=255)
                         aGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATc                                                                                                                  >  4:290397/1‑149 (MQ=255)
                             tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCAATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGc                                                                                                               <  5:195415/148‑1 (MQ=255)
                                gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                              >  8:118508/1‑146 (MQ=255)
                                gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                              <  7:118508/146‑1 (MQ=255)
                                gCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                           >  6:87429/1‑149 (MQ=255)
                                         ggTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTAt                                                                                                  <  5:197715/149‑1 (MQ=255)
                                             gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATgg                                                                                                >  4:53379/1‑147 (MQ=255)
                                              aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                             <  2:83245/149‑1 (MQ=255)
                                              aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                             >  2:325840/1‑149 (MQ=255)
                                                 gCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAggg                                                                                           <  3:184586/148‑1 (MQ=255)
                                                  cAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGGTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTTGGGCCGCTTTGCGGCCCCAATCATCTATGATGCAGATATCCGCCTTGTAGCCTTAAGGGAGGGCa                                                                                         >  7:206949/1‑149 (MQ=255)
                                                   aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCAc                                                                                        >  8:246827/1‑149 (MQ=255)
                                                   aaGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGACTTATGGGAGGGCAc                                                                                        >  8:290695/1‑149 (MQ=255)
                                                    aGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                       <  5:87429/149‑1 (MQ=255)
                                                                    ggCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCAATGAAATGGCCCAGGAAc                                                                       >  5:319166/1‑149 (MQ=255)
                                                                     gCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                      <  6:132910/149‑1 (MQ=255)
                                                                     gCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAAct                                                                      <  2:101166/149‑1 (MQ=255)
                                                                       aTTCTACGACGGCAATGCCTGGGAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaa                                                                                                                                      >  7:49185/1‑83 (MQ=38)
                                                                       aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActct                                                                    >  1:293375/1‑149 (MQ=255)
                                                                       aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAActc                                                                     >  8:155827/1‑148 (MQ=255)
                                                                       aTTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaa                                                                                                                                      <  8:49185/83‑1 (MQ=39)
                                                                        ttCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGACTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTa                                                                   >  7:38778/1‑149 (MQ=255)
                                                                           tACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                           >  8:97070/1‑106 (MQ=255)
                                                                           tACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                           <  7:97070/106‑1 (MQ=255)
                                                                             cgacgGCAATGCCTGTAAGGTTGAAGATAAGCGCTGATGCATTTGTGCTGTGATTTGGTGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGATGGCACTGAAATGGCCCAGGAACTCTACAccc                                                              <  4:59662/149‑1 (MQ=255)
                                                                              gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                             <  1:325840/149‑1 (MQ=255)
                                                                              gacgGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCg                                                             >  8:47803/1‑149 (MQ=255)
                                                                                 ggCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTac                                                          <  7:155827/149‑1 (MQ=255)
                                                                                                     aGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttcttc                                       <  7:230452/148‑1 (MQ=255)
                                                                                                             gcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTc                              <  3:53379/149‑1 (MQ=255)
                                                                                                              cTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCg                             >  3:93902/1‑149 (MQ=255)
                                                                                                                            cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTTTAGCCTGATGGTAGGGCACGGAAATGGCCCAGGAACTCTACACCCGAATCCAAAAGAAACTGATCTTCGCAGGCCTCTCGCTCGAGTCCATg               >  8:292998/1‑149 (MQ=255)
                                                                                                                            cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGTAGGGCACTGAAATGGCGCAGGAACTCTACACCCGTACCAACCAGAAACTGTACTAAGCCGGCATCGCCATCAGGTCCaat               >  8:297249/1‑147 (MQ=255)
                                                                                                                            cTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCAt                <  3:290397/148‑1 (MQ=255)
                                                                                                                               tgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc            <  7:246827/149‑1 (MQ=255)
                                                                                                                               tgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGc             >  6:22829/1‑148 (MQ=255)
                                                                                                                                   ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAgg        >  4:301638/1‑149 (MQ=255)
                                                                                                                                     tgggCCGCAAAGCGGCCCCAATCATTTATGAAGCAGATATACGCCTATAAGACTTATGCCAGGGCACTGAAATGGCACAGGAACTCTACACCCGTAACAACCACAAACTGTTCTTCGAAGGCCTCGCCCACGAGTCCATGTCCaaacc       >  8:176025/2‑145 (MQ=255)
                                                                                                                                          cGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc  >  8:84386/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGGCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAC  >  NC_002947/2443359‑2443644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑