Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,508,777 G→A 16.1% S113L (TCG→TTG) ‡ PP_2203 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,508,7770GA16.1% 98.1 / 9.9 46S113L (TCG→TTG) ‡PP_2203hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/15);  new base A (3/4);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

TAGCAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAG  >  NC_002947/2508633‑2508925
                                                                                                                                                |                                                                                                                                                    
tAGCAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACACCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGcc                                                                                                                                                  >  8:151124/1‑149 (MQ=255)
  gCAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCa                                                                                                                                                <  1:200869/149‑1 (MQ=255)
  gCAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCa                                                                                                                                                >  5:211483/1‑149 (MQ=255)
   cAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCAt                                                                                                                                               <  7:151124/149‑1 (MQ=255)
     gggTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATgg                                                                                                                                             <  6:194564/149‑1 (MQ=255)
     gggTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATgg                                                                                                                                             <  3:101042/149‑1 (MQ=255)
      ggTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATggg                                                                                                                                            >  5:179975/1‑149 (MQ=255)
       gTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGc                                                                                                                                           >  1:194272/1‑149 (MQ=255)
         ggcggcACGTAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGGATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCAt                                                                                                                                         <  2:221178/149‑1 (MQ=255)
          gcggcACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGctc                                                                                                                                         <  2:194272/148‑3 (MQ=255)
                ccAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCGGc                                                                                                                                  <  1:309444/149‑1 (MQ=255)
                 cAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCa                                                                                                                                 >  5:34671/1‑149 (MQ=255)
                  aaaGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCCCCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCACGGCAc                                                                                                                                >  1:1819/1‑149 (MQ=255)
                        gcTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCGGCat                                                                                                                                >  6:174921/1‑142 (MQ=255)
                        gcTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCGGCat                                                                                                                                <  5:174921/143‑2 (MQ=255)
                            ataaaCCGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGCCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGcc                                                                                                                       <  3:181167/146‑1 (MQ=255)
                            agaAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGccc                                                                                                                      >  3:1161/1‑149 (MQ=255)
                                  aGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAgg                                                                                                                >  3:272080/1‑149 (MQ=255)
                                  aGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGAGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGa                                                                                                                  <  3:71276/147‑1 (MQ=255)
                                        ccTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCGGCat                                                                                                                                <  2:40842/127‑2 (MQ=39)
                                        ccTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCATGCCCATGGGCTCGCCCGGCat                                                                                                                                >  1:40842/1‑126 (MQ=39)
                                                   cATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCcag                                                                                               >  3:296544/1‑149 (MQ=255)
                                                                    cACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCGGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcgg                                                                              <  4:86594/149‑1 (MQ=255)
                                                                        gtgtCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTcggccgg                                                                          >  5:308363/1‑149 (MQ=255)
                                                                                 ccATCCGGGGCCACCCCGATCCCCTGGTCGGCATCTTGGGGGTCCCCCATCTCCATGCCGGGCCACCCCTTGGGATTCCCCGCCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGcc                                                                 <  4:206501/149‑1 (MQ=255)
                                                                                    tCCGGGGCCCCCCCGATGACCTGGTAGCCATCCTTGGGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATCCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTc                                                              <  1:284702/149‑1 (MQ=255)
                                                                                          gTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTcgacga                                                         >  3:231392/1‑148 (MQ=255)
                                                                                          ctCACCCCGATGCCGTGTTAGGCTTCCTTGGTGCCACCCATCTCCTTGCGGGGCTCGCCCTTGGGGGTGCCGGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGaa                                                        <  4:185252/148‑1 (MQ=255)
                                                                                                 cGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGCCGATCTTgccg                                                 >  6:75722/1‑149 (MQ=255)
                                                                                                     cagcTGGTAGGCAGCCTTGTGGCCACCCATCTCAAGCCGGGGGGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGa                                             <  5:105906/146‑1 (MQ=255)
                                                                                                      aCCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGAt                                            >  6:158664/1‑149 (MQ=255)
                                                                                                       ccTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATc                                           >  7:56107/1‑149 (MQ=255)
                                                                                                        cTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCa                                          >  7:177021/1‑149 (MQ=255)
                                                                                                                cATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCACATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGACAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTAGACCAACTTTCCGCCGATCACCCCGgtg                                  >  7:268871/1‑149 (MQ=255)
                                                                                                                    cTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTgg                               <  5:308170/148‑1 (MQ=255)
                                                                                                                       gtgGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCt                                                                >  2:77801/1‑112 (MQ=255)
                                                                                                                       gtgGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCt                                                                <  1:77801/112‑1 (MQ=255)
                                                                                                                          gTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCacgacg                        >  5:244259/1‑149 (MQ=255)
                                                                                                                          gTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCacgacg                        >  3:262295/1‑149 (MQ=255)
                                                                                                                           tCACCCATCTCCATGCCGGGCGAGCCCTTGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGc                       <  2:8463/149‑1 (MQ=255)
                                                                                                                               ccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAgg                   >  6:101364/1‑149 (MQ=255)
                                                                                                                                      cttGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGt                <  4:231392/143‑1 (MQ=255)
                                                                                                                                      cATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgcg            >  7:156427/1‑149 (MQ=255)
                                                                                                                                        tGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGgcgc           >  2:304701/1‑148 (MQ=255)
                                                                                                                                            gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGc      >  2:118144/1‑149 (MQ=255)
                                                                                                                                               cGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGa   <  4:296544/149‑1 (MQ=255)
                                                                                                                                                gAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg  <  8:177021/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TAGCAGGGTGGCGGCACCAAAGGCGCTGAGAAACAGCGCCCCTAGGCTGAGCATCAGTAGTTGGCGACCACGGTGTCCTGGCCATCCTGGGTCACCCCGATGACCTGGTAGGCATCCTTGTGGTCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGGTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAG  >  NC_002947/2508633‑2508925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑