Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,511,676 T→C 6.3% T19A (ACG→GCG) ‡ copA‑I ← copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,511,6760TC 6.3% 180.5 / 2.3 63T19A (ACG→GCG) ‡copA‑Icopper resistance protein A
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

CTGTTGTTGACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCAGCTTGCTGCTTC  >  NC_002947/2511531‑2511823
                                                                                                                                                 |                                                                                                                                                   
cTGTTGTTGACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGTCCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCt                                                                                                                                                  >  2:230143/1‑149 (MQ=255)
  gttgttGACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGAGCGGAGTCTGGCCAATGAACAGGTCGATATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGATgg                                                                                                                                                >  7:232183/1‑149 (MQ=255)
   ttgttgACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGGCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTggc                                                                                                                                               <  3:173776/149‑1 (MQ=255)
     ggtgACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAAGGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTggcg                                                                                                                                              <  5:41298/146‑1 (MQ=255)
         aCGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGTCCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGTGgcgc                                                                                                                                          <  1:230143/148‑1 (MQ=255)
            gTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAg                                                                                                                                      >  5:85637/1‑149 (MQ=255)
            gTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAg                                                                                                                                      >  2:250736/1‑149 (MQ=255)
                gTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCt                                                                                                                                  >  6:260082/1‑149 (MQ=255)
                 tGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCtt                                                                                                                                 >  2:290691/1‑149 (MQ=255)
                    ggTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGa                                                                                                                              <  1:290691/149‑1 (MQ=255)
                     gTACGCGGTCGGCCTGTGATGTTGACCGGAGGCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCGCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGAc                                                                                                                             <  2:287344/149‑1 (MQ=255)
                           ggTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACg                                                                                                                        >  3:96407/1‑148 (MQ=255)
                           ggTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCACGCTggcgg                                                                                                                                             >  4:153012/1‑127 (MQ=255)
                           ggTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCACGCTggcgg                                                                                                                                             <  3:153012/127‑1 (MQ=255)
                            gTCGGCCTGTGATGTTGACCAGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTa                                                                                                                      >  2:173425/1‑149 (MQ=255)
                               ggCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGACCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTAcgg                                                                                                                   >  3:123957/1‑149 (MQ=255)
                                 ccTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCAAGCGTGCTGCCGGCGCCCAGGCCTATGACAAACGTAcggcg                                                                                                                 >  4:20843/1‑149 (MQ=255)
                                  cTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTAcggcgg                                                                                                                <  6:85637/149‑1 (MQ=255)
                                     tgATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGTGCCCAGGCCTTTGACAAACGTAcgg                                                                                                                   <  3:208305/143‑1 (MQ=255)
                                     tgATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGTGCCCAGGCCTTTGACAAACGTAcgg                                                                                                                   >  4:208305/1‑143 (MQ=255)
                                     tgATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGTGCCCAGGCCTTTGACAAACGTAcgg                                                                                                                   >  8:302954/1‑143 (MQ=255)
                                     tgATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGTGCCCAGGCCTTTGACAAACGTAcgg                                                                                                                   <  7:302954/143‑1 (MQ=255)
                                       aTGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCACGCTggcgg                                                                                                                                             >  6:75928/1‑115 (MQ=255)
                                       aTGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCACGCTggcgg                                                                                                                                             <  5:75928/115‑1 (MQ=255)
                                        tGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGACCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAgggg                                                                                                          <  4:123957/149‑1 (MQ=255)
                                          ttGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGtt                                                                                                        <  4:93725/149‑1 (MQ=255)
                                             aCCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTgc                                                                                                      <  4:164744/148‑1 (MQ=255)
                                             aCCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTgc                                                                                                      <  5:260082/148‑1 (MQ=255)
                                             aCCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTgc                                                                                                      <  7:292028/148‑1 (MQ=255)
                                             aCCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCCTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTgcg                                                                                                     >  4:247316/1‑149 (MQ=255)
                                               cGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTgcgc                                                                                                    >  8:144549/1‑148 (MQ=255)
                                                ggAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCaa                                                                                                  <  1:49739/149‑1 (MQ=255)
                                                ggAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCaa                                                                                                  <  2:55574/149‑1 (MQ=255)
                                                  aGTCTGCCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCACGCCATCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACa                                                                                                <  3:20843/149‑1 (MQ=255)
                                                    tCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATg                                                                                              <  1:250736/149‑1 (MQ=255)
                                                        gCCAATGAACAGCTCGAAATTCTGGCCCGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGa                                                                                          <  8:232183/149‑1 (MQ=255)
                                                                       gAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGaa                                                                            >  3:61541/1‑148 (MQ=255)
                                                                        aaaTGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGaa                                                                            >  4:242777/1‑147 (MQ=255)
                                                                        aaaTGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATa                                                                          <  1:173425/149‑1 (MQ=255)
                                                                              tGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACg                                                                    >  7:150822/1‑149 (MQ=255)
                                                                                 ccggccAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCa                                                                      >  8:152273/1‑144 (MQ=255)
                                                                                 ccggccAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCa                                                                      <  7:152273/144‑1 (MQ=255)
                                                                                     ccAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGAGAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCg                                                             <  3:247316/149‑1 (MQ=255)
                                                                                     ccAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCg                                                             <  7:144549/149‑1 (MQ=255)
                                                                                         gTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTgc                                                         >  2:114130/1‑149 (MQ=255)
                                                                                         gTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTgc                                                         >  1:254/1‑149 (MQ=255)
                                                                                          tctcGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTgcc                                                        >  2:26638/1‑149 (MQ=255)
                                                                                          tctcGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTgcc                                                        >  6:313632/1‑149 (MQ=255)
                                                                                             cGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTgccgcc                                                     >  3:274999/1‑149 (MQ=255)
                                                                                                ccTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCctgc                                                  >  4:166877/1‑149 (MQ=255)
                                                                                                     aGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCa                                             <  4:96407/149‑1 (MQ=255)
                                                                                                       cGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAgg                                           >  4:119834/1‑149 (MQ=255)
                                                                                                            tGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCt                                      >  7:180537/1‑149 (MQ=255)
                                                                                                              gACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCt                                      <  4:60200/147‑1 (MQ=255)
                                                                                                               aCCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAg                                   <  8:150822/149‑1 (MQ=255)
                                                                                                                                aCCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGtgtt                  <  8:180537/149‑1 (MQ=255)
                                                                                                                                 cccAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGtgtt                  <  3:119834/148‑1 (MQ=255)
                                                                                                                                  ccAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGtgttgt                >  5:106505/1‑149 (MQ=255)
                                                                                                                                    aGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCa              <  3:166877/149‑1 (MQ=255)
                                                                                                                                     ggCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGtgtt                  >  1:152498/1‑144 (MQ=255)
                                                                                                                                     ggCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCAg             <  2:254/149‑1 (MQ=255)
                                                                                                                                            cAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCAGCTtgctg      >  7:192720/1‑149 (MQ=255)
                                                                                                                                                gTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCAGCTTGCTGCTtc  <  4:61541/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGTTGTTGACGGTCAGTGCGGTACGCGGTCGGCCTGTGATGTTGACCGGAGTCTGGCCAATGAACAGCTCGAAATGCTGGCCGGCCAGGTCTCGGCCTTCAGCGGCCTGGACCAGCGGGCGCCACAGACCCAGGCCCGCCAGCGTGCTGGCGGCGCCCAGGCCTTTGACAAACGTACGGCGGGAGGGGTTGCGCAACATGGCGAGGCCTCATGCGGAATAGCCACGATGTTCGCTGCCGCCTGCTGTCAGGTAGCTGAGGTGCACATTACAGTGTTGTCAGCTTGCTGCTTC  >  NC_002947/2511531‑2511823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑