Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,578,982 T→C 8.5% L131P (CTG→CCG)  PP_2260 → glycerol‑phosphate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,578,9820TC 8.5% 126.9 / 3.3 47L131P (CTG→CCG) PP_2260glycerol‑phosphate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (18/25);  new base C (2/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

AATTCATCAATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCT  >  NC_002947/2578835‑2579123
                                                                                                                                                   |                                                                                                                                             
aaTTCATCAATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTg                                                                                                                                              <  8:108391/149‑1 (MQ=255)
aaTTCATCAATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTg                                                                                                                                              >  2:48916/1‑149 (MQ=255)
        aaTTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctc                                                                                                                                      <  1:48916/149‑1 (MQ=255)
        aaTTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctc                                                                                                                                      >  3:38736/1‑149 (MQ=255)
          ttACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGTCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctcag                                                                                                                                    >  2:119145/1‑147 (MQ=255)
             cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGt                                                                                                                                  <  1:243007/148‑1 (MQ=255)
             cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACCGGAGAGCTCCAGt                                                                                                                                  <  8:99453/148‑1 (MQ=255)
              ccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTgg                                                                                                                                >  6:325452/1‑149 (MQ=255)
               cGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGc                                                                                                                               >  6:275399/1‑149 (MQ=255)
                 aCCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCAGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCca                                                                                                                             <  6:129888/149‑1 (MQ=255)
                      gAGCGTGTTCGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCcagca                                                                                                                          <  1:12084/147‑1 (MQ=255)
                       aGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCagcagc                                                                                                                        <  4:38736/148‑1 (MQ=255)
                             tACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCAc                                                                                                                 >  1:100282/1‑149 (MQ=255)
                                  aaaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGa                                                                                                            >  2:99319/1‑149 (MQ=255)
                                   aaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGCTGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGAt                                                                                                           <  5:50546/149‑1 (MQ=255)
                                        cgcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCCGCAACGCACGGCGATGGc                                                                                                        >  7:293207/1‑147 (MQ=255)
                                         gcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCa                                                                                                      <  5:58423/148‑1 (MQ=255)
                                          cctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAgg                                                                                                    <  5:112343/149‑1 (MQ=255)
                                           ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCTGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                   <  8:293207/149‑1 (MQ=255)
                                           ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                   >  3:317294/1‑149 (MQ=255)
                                           ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                   <  3:52327/149‑1 (MQ=255)
                                              gccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCa                                                                                                 <  2:100282/148‑1 (MQ=255)
                                               ccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACt                                                                                               <  6:147376/149‑1 (MQ=255)
                                                     cgcCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCaa                                                                                         <  3:103533/149‑1 (MQ=255)
                                                       ccAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAgg                                                                                       <  2:94393/149‑1 (MQ=255)
                                                        cAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGa                                                                                      <  5:275399/149‑1 (MQ=255)
                                                                cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCGGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                              <  8:220330/149‑1 (MQ=255)
                                                                cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                              <  1:99319/149‑1 (MQ=255)
                                                                             cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgc                                                                 >  4:241799/1‑148 (MQ=255)
                                                                             cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                 >  5:110994/1‑149 (MQ=255)
                                                                             cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                 >  2:236292/1‑149 (MQ=255)
                                                                               gATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgccg                                                               >  7:147031/1‑149 (MQ=255)
                                                                                   cggcggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGc                                                            >  4:310871/1‑148 (MQ=255)
                                                                                       ggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTg                                                       <  1:236292/149‑1 (MQ=255)
                                                                                                  gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                            <  3:310871/149‑1 (MQ=255)
                                                                                                  gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                            <  6:110994/149‑1 (MQ=255)
                                                                                                          cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                              >  1:294850/1‑139 (MQ=255)
                                                                                                          cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                              <  2:294850/139‑1 (MQ=255)
                                                                                                                        tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                           >  7:184620/1‑128 (MQ=255)
                                                                                                                        tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                           <  8:184620/128‑1 (MQ=255)
                                                                                                                                    tGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTg          >  5:178776/1‑149 (MQ=255)
                                                                                                                                            tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg      <  6:109934/143‑1 (MQ=255)
                                                                                                                                            tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg      >  5:109934/3‑145 (MQ=255)
                                                                                                                                            tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt  >  3:50538/3‑149 (MQ=255)
                                                                                                                                            cTGCCACTGGAGGTGTCCGGTGGCCAGCAGCATCGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt  <  8:61979/149‑1 (MQ=255)
                                                                                                                                                cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg             <  6:127587/134‑1 (MQ=255)
                                                                                                                                                cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg             >  5:127587/1‑134 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AATTCATCAATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCT  >  NC_002947/2578835‑2579123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑