Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,578,988 T→G 9.3% L133R (CTC→CGC) ‡ PP_2260 → glycerol‑phosphate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,578,9880TG 9.3% 125.7 / 4.6 43L133R (CTC→CGC) ‡PP_2260glycerol‑phosphate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (16/23);  new base G (2/2);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCG  >  NC_002947/2578843‑2579135
                                                                                                                                                 |                                                                                                                                                   
aaTTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctc                                                                                                                                                  <  1:48916/149‑1 (MQ=255)
aaTTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctc                                                                                                                                                  >  3:38736/1‑149 (MQ=255)
  ttACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGTCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCtctcag                                                                                                                                                >  2:119145/1‑147 (MQ=255)
     cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGt                                                                                                                                              <  1:243007/148‑1 (MQ=255)
     cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACCGGAGAGCTCCAGt                                                                                                                                              <  8:99453/148‑1 (MQ=255)
      ccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTgg                                                                                                                                            >  6:325452/1‑149 (MQ=255)
       cGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGc                                                                                                                                           >  6:275399/1‑149 (MQ=255)
         aCCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCAGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCca                                                                                                                                         <  6:129888/149‑1 (MQ=255)
              gAGCGTGTTCGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCcagca                                                                                                                                      <  1:12084/147‑1 (MQ=255)
               aGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCagcagc                                                                                                                                    <  4:38736/148‑1 (MQ=255)
                     tACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCAc                                                                                                                             >  1:100282/1‑149 (MQ=255)
                          aaaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGa                                                                                                                        >  2:99319/1‑149 (MQ=255)
                           aaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGCTGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGAt                                                                                                                       <  5:50546/149‑1 (MQ=255)
                                cgcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCCGCAACGCACGGCGATGGc                                                                                                                    >  7:293207/1‑147 (MQ=255)
                                 gcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCa                                                                                                                  <  5:58423/148‑1 (MQ=255)
                                  cctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAgg                                                                                                                <  5:112343/149‑1 (MQ=255)
                                   ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCTGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                               <  8:293207/149‑1 (MQ=255)
                                   ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                               >  3:317294/1‑149 (MQ=255)
                                   ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                               <  3:52327/149‑1 (MQ=255)
                                      gccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCa                                                                                                             <  2:100282/148‑1 (MQ=255)
                                       ccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACt                                                                                                           <  6:147376/149‑1 (MQ=255)
                                             cgcCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCaa                                                                                                     <  3:103533/149‑1 (MQ=255)
                                               ccAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAgg                                                                                                   <  2:94393/149‑1 (MQ=255)
                                                cAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGa                                                                                                  <  5:275399/149‑1 (MQ=255)
                                                        cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCGGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                                          <  8:220330/149‑1 (MQ=255)
                                                        cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                                          <  1:99319/149‑1 (MQ=255)
                                                                     cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgc                                                                             >  4:241799/1‑148 (MQ=255)
                                                                     cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                             >  5:110994/1‑149 (MQ=255)
                                                                     cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                             >  2:236292/1‑149 (MQ=255)
                                                                       gATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgccg                                                                           >  7:147031/1‑149 (MQ=255)
                                                                           cggcggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGc                                                                        >  4:310871/1‑148 (MQ=255)
                                                                               ggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTg                                                                   <  1:236292/149‑1 (MQ=255)
                                                                                          gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                                        <  3:310871/149‑1 (MQ=255)
                                                                                          gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                                        <  6:110994/149‑1 (MQ=255)
                                                                                                  cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                                          >  1:294850/1‑139 (MQ=255)
                                                                                                  cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                                          <  2:294850/139‑1 (MQ=255)
                                                                                                                tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                                       >  7:184620/1‑128 (MQ=255)
                                                                                                                tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                                       <  8:184620/128‑1 (MQ=255)
                                                                                                                            tGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTg                      >  5:178776/1‑149 (MQ=255)
                                                                                                                                    tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg                  <  6:109934/143‑1 (MQ=255)
                                                                                                                                    tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg                  >  5:109934/3‑145 (MQ=255)
                                                                                                                                    tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt              >  3:50538/3‑149 (MQ=255)
                                                                                                                                    cTGCCACTGGAGGTGTCCGGTGGCCAGCAGCATCGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt              <  8:61979/149‑1 (MQ=255)
                                                                                                                                        cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg                         <  6:127587/134‑1 (MQ=255)
                                                                                                                                        cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg                         >  5:127587/1‑134 (MQ=255)
                                                                                                                                             gAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCgcggc     >  3:130113/1‑149 (MQ=255)
                                                                                                                                                ctctCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGgcgcg  >  5:183346/1‑149 (MQ=255)
                                                                                                                                                ctctCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGAAGAGCCATTGGTCAACCTCGACTACAAGTTCCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTtcgcgtcgcg  >  7:241271/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AATTACCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCG  >  NC_002947/2578843‑2579135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑