Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,578,993 G→A 10.4% G135S (GGT→AGT)  PP_2260 → glycerol‑phosphate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,578,9930GA10.4% 128.5 / 5.2 48G135S (GGT→AGT) PP_2260glycerol‑phosphate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base A (2/3);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

CCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCGCCA  >  NC_002947/2578848‑2579138
                                                                                                                                                 |                                                                                                                                                 
cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGt                                                                                                                                                 <  1:243007/148‑1 (MQ=255)
cccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACCGGAGAGCTCCAGt                                                                                                                                                 <  8:99453/148‑1 (MQ=255)
 ccGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTgg                                                                                                                                               >  6:325452/1‑149 (MQ=255)
  cGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGc                                                                                                                                              >  6:275399/1‑149 (MQ=255)
    aCCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCAGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCca                                                                                                                                            <  6:129888/149‑1 (MQ=255)
         gAGCGTGTTCGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCcagca                                                                                                                                         <  1:12084/147‑1 (MQ=255)
          aGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCagcagc                                                                                                                                       <  4:38736/148‑1 (MQ=255)
                tACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCAc                                                                                                                                >  1:100282/1‑149 (MQ=255)
                     aaaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGa                                                                                                                           >  2:99319/1‑149 (MQ=255)
                      aaCATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGCTGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGAt                                                                                                                          <  5:50546/149‑1 (MQ=255)
                           cgcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCCGCAACGCACGGCGATGGc                                                                                                                       >  7:293207/1‑147 (MQ=255)
                            gcctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCa                                                                                                                     <  5:58423/148‑1 (MQ=255)
                             cctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAgg                                                                                                                   <  5:112343/149‑1 (MQ=255)
                              ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCTGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                                  <  8:293207/149‑1 (MQ=255)
                              ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                                  >  3:317294/1‑149 (MQ=255)
                              ctcgccGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAggg                                                                                                                  <  3:52327/149‑1 (MQ=255)
                                 gccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCa                                                                                                                <  2:100282/148‑1 (MQ=255)
                                  ccgcTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACt                                                                                                              <  6:147376/149‑1 (MQ=255)
                                        cgcCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCaa                                                                                                        <  3:103533/149‑1 (MQ=255)
                                          ccAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAgg                                                                                                      <  2:94393/149‑1 (MQ=255)
                                           cAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGa                                                                                                     <  5:275399/149‑1 (MQ=255)
                                                   cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCGGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                                             <  8:220330/149‑1 (MQ=255)
                                                   cATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGt                                                                                             <  1:99319/149‑1 (MQ=255)
                                                                cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgc                                                                                >  4:241799/1‑148 (MQ=255)
                                                                cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                                >  2:236292/1‑149 (MQ=255)
                                                                cAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcga                                                                                >  5:110994/1‑149 (MQ=255)
                                                                  gATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTcgccg                                                                              >  7:147031/1‑149 (MQ=255)
                                                                      cggcggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGc                                                                           >  4:310871/1‑148 (MQ=255)
                                                                          ggcggGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTg                                                                      <  1:236292/149‑1 (MQ=255)
                                                                                     gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                                           <  3:310871/149‑1 (MQ=255)
                                                                                     gAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGa                                                           <  6:110994/149‑1 (MQ=255)
                                                                                             cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                                             <  2:294850/139‑1 (MQ=255)
                                                                                             cGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTc                                                             >  1:294850/1‑139 (MQ=255)
                                                                                                           tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                                          <  8:184620/128‑1 (MQ=255)
                                                                                                           tCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGAc                                                          >  7:184620/1‑128 (MQ=255)
                                                                                                                       tGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTg                         >  5:178776/1‑149 (MQ=255)
                                                                                                                               tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg                     <  6:109934/143‑1 (MQ=255)
                                                                                                                               tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGcgcg                     >  5:109934/3‑145 (MQ=255)
                                                                                                                               tggCCACCGGAGAGCTCCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt                 >  3:50538/3‑149 (MQ=255)
                                                                                                                               cTGCCACTGGAGGTGTCCGGTGGCCAGCAGCATCGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCt                 <  8:61979/149‑1 (MQ=255)
                                                                                                                                   cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg                            <  6:127587/134‑1 (MQ=255)
                                                                                                                                   cACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAg                            >  5:127587/1‑134 (MQ=255)
                                                                                                                                        gAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCgcggc        >  3:130113/1‑149 (MQ=255)
                                                                                                                                           ctctCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGgcgcg     >  5:183346/1‑149 (MQ=255)
                                                                                                                                           ctctCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGAAGAGCCATTGGTCAACCTCGACTACAAGTTCCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTtcgcgtcgcg     >  7:241271/1‑149 (MQ=255)
                                                                                                                                             ctcCAGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCGcc   <  4:50538/149‑1 (MQ=255)
                                                                                                                                              tcCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCGCca  >  3:313313/1‑149 (MQ=255)
                                                                                                                                              tcCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCGCca  >  5:271225/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCCGACCTTGAGCGTGTACGAAAACATCGCCTCGCCGCTGCGCCAGGCGCGCATGGCCGAGGACCAGATCCGGCGGCGGGTGCAGGAAACTGCCGAGATGCTGCGCATCGAAACCTACCTGCAACGCCTGCCACTGGAGCTCTCCGGTGGCCAGCAGCAACGCACGGCGATGGCCAGGGCACTGGTCAAGGATGCCTCGTTGATCCTCTTCGACGAGCCATTGGTCAACCTCGACTACAAGTTGCGCGAAGGCCTGCGCCAGGAGCTGCGCGAGCTGTTCGCGGCGCGCCA  >  NC_002947/2578848‑2579138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑