Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,631,143 T→A 9.3% L203Q (CTG→CAG)  lon‑II → DNA‑binding ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,631,1430TA 9.3% 142.5 / 4.5 53L203Q (CTG→CAG) lon‑IIDNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base T (21/27);  new base A (2/3);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

ATCGACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAA  >  NC_002947/2630998‑2631287
                                                                                                                                                 |                                                                                                                                                
aTCGACGAGCCAGGGCGCCTGGTCGACACCATGGACGCGCACATGGCCCAGAAGATCGAGCAGAAGCAGGAAATTCTAGAAATCGTCGACTTGAATACCCGTGTCCAGCACGTCCTGGCGCTGCTGGAAGCAGAAATCCACCTCCTg                                                                                                                                                 >  8:196856/1‑147 (MQ=255)
   gacgaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGt                                                                                                                                            <  1:136086/149‑1 (MQ=255)
    acgaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTc                                                                                                                                           <  3:162564/149‑1 (MQ=255)
     cgaGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCg                                                                                                                                          <  8:218/149‑1 (MQ=255)
        gCCAGGGCGCCTGGTCGACACCACGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGatt                                                                                                                                       <  7:109575/149‑3 (MQ=255)
           aGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAgc                                                                                                                                    >  2:123831/1‑149 (MQ=255)
           aGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAAACGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGatttcg                                                                                                                                    <  1:102798/149‑6 (MQ=255)
              gcgcCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCa                                                                                                                                 >  3:254678/1‑149 (MQ=255)
                         acacCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                      <  2:109973/149‑1 (MQ=255)
                         acacCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                      <  5:61351/149‑1 (MQ=255)
                             cATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCTCATTCTCGGCCGGGTc                                                                                                                   >  6:240139/1‑148 (MQ=255)
                             cATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTc                                                                                                                   >  1:236432/1‑148 (MQ=255)
                             cATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTc                                                                                                                   >  5:55564/1‑148 (MQ=255)
                                   cgcgCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGt                                                                                                                    <  1:60225/141‑1 (MQ=255)
                                   cgcgCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGt                                                                                                                    >  2:60225/1‑141 (MQ=255)
                                    gcgcACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCGGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCa                                                                                                           <  6:213548/149‑1 (MQ=255)
                                       cacaTGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGAt                                                                                                        <  4:218435/149‑1 (MQ=255)
                                         caTGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATgg                                                                                                      <  2:236432/149‑1 (MQ=255)
                                          aTGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATg                                                                                                       >  8:144596/1‑147 (MQ=255)
                                              cccTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAgcgc                                                                                                 <  7:195220/149‑1 (MQ=255)
                                               ccTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCCGCCgg                                                                                                                      <  6:322238/127‑1 (MQ=255)
                                               ccTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCCCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCCGCCgg                                                                                                                      >  5:322238/1‑127 (MQ=255)
                                                     gATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGcagccagc                                                                                          <  8:256/149‑1 (MQ=255)
                                                        cGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAgcg                                                                                         >  6:274831/1‑147 (MQ=255)
                                                        cGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAgcg                                                                                         <  5:274831/147‑1 (MQ=255)
                                                            cAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                      >  5:69264/1‑114 (MQ=255)
                                                            cAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                      <  6:69264/114‑1 (MQ=255)
                                                            cAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGta                                                                                   >  6:263375/1‑149 (MQ=255)
                                                            cAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACCTGCTCGCGCTGCAGCATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATCGAGCGCAGCCAGAGCGAGt                                                                                    >  7:140916/1‑148 (MQ=255)
                                                                   aGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCt                                                                             >  4:22122/1‑148 (MQ=255)
                                                                     gAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAgcgc                                                                                        <  6:34142/135‑1 (MQ=255)
                                                                     gAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAgcgc                                                                                        >  5:34142/1‑135 (MQ=255)
                                                                      aaaTTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAAt                                                                         <  1:123831/149‑1 (MQ=255)
                                                                         ttCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAg                                                                      <  6:26756/149‑1 (MQ=255)
                                                                          tCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAg                                                                      >  1:207383/1‑148 (MQ=255)
                                                                             tGAAATCGTCGACTTGACTACCCTTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCTAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAg                                                                   <  7:196856/148‑1 (MQ=255)
                                                                                    gtcgACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAgg                                                            <  7:144596/148‑1 (MQ=255)
                                                                                                 cccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCagaaagaa                                              >  1:227128/1‑149 (MQ=255)
                                                                                                  ccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAAt                                                                         >  8:241442/1‑121 (MQ=255)
                                                                                                  ccGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAAt                                                                         <  7:241442/121‑1 (MQ=255)
                                                                                                  ccGTGTCGAGCACGTGCTGGCGATGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCTGGTCAAGAAGCAGATGGAGCGCAGCCAGCGAGAGTACTACCTGAATGAGCAGATGAAGGCCAtt                                                       <  8:207940/139‑1 (MQ=255)
                                                                                                  cagtgtCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTACTGCAGGTCGAAAAGCGCATTCGCGGCCTGGGAAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCAtt                                                       >  7:207940/3‑139 (MQ=255)
                                                                                                     tgtCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTg                                          <  3:22122/149‑1 (MQ=255)
                                                                                                           gCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATg                                    <  4:254678/149‑1 (MQ=255)
                                                                                                            cACGTGCTGGCGCTTCTGGATGCCTTAATCGACCTCCTGCAGGTCGAAAAGCGCATTCTCGGCCTGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATgg                                   <  8:140916/149‑1 (MQ=255)
                                                                                                            cACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATgg                                   <  5:263375/149‑1 (MQ=255)
                                                                                                               gTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGa                                <  4:249746/149‑1 (MQ=255)
                                                                                                                               aTGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGaa                <  2:227128/149‑1 (MQ=255)
                                                                                                                                   gcttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTgg                                         <  3:84941/115‑1 (MQ=255)
                                                                                                                                   gcttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTgg                                         >  4:84941/6‑120 (MQ=255)
                                                                                                                                   cGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTc             >  8:269544/1‑148 (MQ=255)
                                                                                                                                     ttttCGACCTGCAGGAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCg            >  2:207863/4‑147 (MQ=255)
                                                                                                                                         cGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTAACTGAATGAGCAGATCAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAAAGAAGTCGAagag       >  2:291859/1‑148 (MQ=255)
                                                                                                                                             ctcctGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGa   >  3:162143/1‑148 (MQ=255)
                                                                                                                                              tcctGCAGGTCGAAAAGCGCATTCGCCGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGaa  >  6:158746/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCGACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAA  >  NC_002947/2630998‑2631287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑