Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,651,276 G→C 9.1% intergenic (+41/+29) PP_2323 → / ← aroF‑I hypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,651,2760GC 9.1% 166.0 / 6.4 55intergenic (+41/+29)PP_2323/aroF‑Ihypothetical protein/tyr‑sensitive phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (30/20);  new base C (2/3);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

TCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGCA  >  NC_002947/2651136‑2651420
                                                                                                                                            |                                                                                                                                                 
tctGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGc                                                                                                                                           >  4:169024/1‑149 (MQ=255)
  tGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGACAGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGCCAGTGCCCGGCGt                                                                                                                                         >  6:22827/1‑149 (MQ=255)
     aGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTCCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGtttt                                                                                                                                      <  1:132139/149‑1 (MQ=255)
       tACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGtttttt                                                                                                                                    <  7:102477/149‑2 (MQ=255)
             gAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTccc                                                                                                                              >  6:329530/1‑149 (MQ=255)
             gAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGt                                                                                                                                         <  8:157191/138‑1 (MQ=255)
             gAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCACTGCCCGGCGt                                                                                                                                         >  7:157191/1‑138 (MQ=255)
             gAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGATGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTccc                                                                                                                              >  2:288923/1‑149 (MQ=255)
                                            gCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGgcag                                                                                               >  7:219217/1‑149 (MQ=255)
                                              tCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGgcag‑ca                                                                                            >  3:49253/1‑149 (MQ=255)
                                               cATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGgcag‑ca                                                                                            >  8:46632/1‑148 (MQ=255)
                                                   tgtgGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATc                                                                                        <  8:219711/148‑1 (MQ=255)
                                                       gAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCtt                                                                                     >  1:292772/1‑147 (MQ=255)
                                                       gAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTg                                                                                    <  6:49337/148‑1 (MQ=255)
                                                          cGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGc                                                                                 <  3:162507/148‑1 (MQ=255)
                                                            ttACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCg                                                                                                   >  4:296192/1‑129 (MQ=255)
                                                            ttACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCg                                                                                                   <  3:296192/129‑1 (MQ=255)
                                                               cATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGgc                                                                            <  5:267263/148‑1 (MQ=255)
                                                               cATGGAGCGCCAGCAGAGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGTGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGgc                                                                            <  4:257225/148‑1 (MQ=255)
                                                                 tGGAGCGACAGCAGCGGCATTCGAGCAAGGCCTGAGATGACGACAGGAACCAGACACAACAAAAAAAGCCGGGCAGTGCCCGGCTTTTTTAATTACCGCAATGTTCAGACTCGATTACGCTGCTGCAG‑CAAATCATTTAGCTTGgcatg                                                                         >  4:76773/1‑149 (MQ=255)
                                                                  ggAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCtt                                                                               >  1:206816/1‑142 (MQ=255)
                                                                  ggAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCtt                                                                               <  2:206816/142‑1 (MQ=255)
                                                                     gcgcCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGcatgcatg                                                                     <  3:169024/149‑1 (MQ=255)
                                                                        ccAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAGCCACATCCTTGAGCTTGGCATGCAtgct                                                                   <  1:168586/149‑1 (MQ=255)
                                                                         cagcagCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATc                                                                                        >  4:177630/1‑126 (MQ=255)
                                                                         cagcagCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATc                                                                                        <  3:177630/126‑1 (MQ=255)
                                                                           gcagcGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTgcg                                                                >  7:1671/1‑148 (MQ=255)
                                                                               cGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAggg                                                           <  3:268900/149‑1 (MQ=255)
                                                                                ggCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGt                                                          >  5:231165/1‑149 (MQ=255)
                                                                                 gCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCTGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGtt                                                         <  1:288923/149‑1 (MQ=255)
                                                                                 gCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGtt                                                         <  7:46632/149‑1 (MQ=255)
                                                                                  cATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGttt                                                        >  3:262182/1‑149 (MQ=255)
                                                                                   aTTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGtttt                                                       <  6:329082/149‑1 (MQ=255)
                                                                                       gAGCACGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTGGAGCTAGGCATGCATGCTGCGCAGGGATTGCTCg                                                   >  4:247921/1‑149 (MQ=255)
                                                                                       gAGCAAGCCCTGAGGTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTtctc                                                    <  5:1692/148‑1 (MQ=255)
                                                                                         gCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCgg                                                  >  2:269673/1‑148 (MQ=255)
                                                                                         gCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCgg                                                  >  5:90534/1‑148 (MQ=255)
                                                                                          cAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCgg                                                  >  4:213611/1‑147 (MQ=255)
                                                                                              gCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGcc                                             >  8:134556/1‑148 (MQ=255)
                                                                                                cTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGAc                                          >  3:181034/1‑149 (MQ=255)
                                                                                                cTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGAc                                          >  8:1206/1‑149 (MQ=255)
                                                                                                     cTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTc                                     <  7:134556/149‑1 (MQ=255)
                                                                                                          gacAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCTATg                                 >  2:105326/1‑148 (MQ=255)
                                                                                                            cAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAg                              <  5:22827/149‑1 (MQ=255)
                                                                                                             aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAgg                             >  2:272625/1‑149 (MQ=255)
                                                                                                             aGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAgg                             >  4:231722/1‑149 (MQ=255)
                                                                                                                    aGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGt                      <  3:231722/149‑1 (MQ=255)
                                                                                                                     gACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTg                     >  3:187848/1‑149 (MQ=255)
                                                                                                                          aacaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGa                >  5:39317/1‑149 (MQ=255)
                                                                                                                           acaaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGa                >  6:127180/1‑148 (MQ=255)
                                                                                                                            caaAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGaca              >  1:299580/1‑149 (MQ=255)
                                                                                                                                aaaCGCCGGGCACTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGc           >  7:153425/1‑148 (MQ=255)
                                                                                                                                    ggcGGGAAGTGACCTGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCTTCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCATGTTTTTCTCGTTAGCCGACCAGTCGATGCAGGCGTCCGTGATCTACACGCCATAc      <  3:76773/147‑1 (MQ=255)
                                                                                                                                       gggCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGc   <  3:213611/149‑1 (MQ=255)
                                                                                                                                         gCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGCa  >  5:32760/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCTGGAGTACGCCGAGCAGATGGGGTACACGGTGATCCCGTCTTGCTCATATGTGGAGCGTTACATGGAGCGCCAGCAGCGGCATTCGAGCAAGGCCTGAGCTGACGACAGGCACCAGACACAACAAAAAACGCCGGGCAGTGCCCGGCGTTTTTCATTCCCGCAATGTTCAGCCGCGCTTACGCTGCGGCAG‑CACATCCTTGAGCTTGGCATGCATGCTGCGCAGGGTTTTCTCGGTAGCCGACCAGTCGATGCAGGCGTCCGTGATCGACACGCCATACTGCA  >  NC_002947/2651136‑2651420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑