Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,783,192 C→T 8.5% L77L (CTG→CTA) ‡ PP_2436 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,783,1920CT 8.5% 129.6 / 2.2 47L77L (CTG→CTA) ‡PP_2436LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

ACTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGC  >  NC_002947/2783046‑2783331
                                                                                                                                                  |                                                                                                                                            
aCTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAcagcgccag                                                                                                                                            >  4:293787/1‑149 (MQ=255)
  tGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAcagcgccagcg                                                                                                                                          >  3:82203/1‑149 (MQ=255)
      ggCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑Gtt                                                                                                                                     <  3:293787/149‑1 (MQ=255)
         gcCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGACAGCGC‑GTTgcg                                                                                                                                  >  8:133543/1‑149 (MQ=255)
         gcCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAC‑GCGCTGGCGCTGTTgcg                                                                                                                                  <  4:45612/149‑1 (MQ=255)
               cggcgCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTGCGCAAC‑GCGCTGGCGCTGTTGCGgacatc                                                                                                                            <  4:33605/149‑3 (MQ=255)
                     aGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCgat                                                                                                                      <  2:217486/149‑1 (MQ=255)
                         gcTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGatgatg                                                                                                                  >  1:159325/1‑149 (MQ=255)
                                           aGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGtgtg                                                                                                >  1:39814/1‑149 (MQ=255)
                                            gATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTgg                                                                                               <  2:39814/149‑1 (MQ=255)
                                             aTGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTggg                                                                                              <  4:297666/149‑1 (MQ=255)
                                                aCCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCAc                                                                                           <  2:159325/149‑1 (MQ=255)
                                                       cGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCt                                                                                    >  1:285370/1‑149 (MQ=255)
                                                         accgaggcggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCtt                                                                                   <  1:328104/141‑1 (MQ=255)
                                                           cAGGTCGGTCATACTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTgcg                                                                                >  5:77178/1‑149 (MQ=255)
                                                               tcggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGt                                                                            <  5:64558/149‑1 (MQ=255)
                                                                cggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTa                                                                           >  1:13651/1‑149 (MQ=255)
                                                                 ggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTa                                                                           <  6:216252/148‑1 (MQ=255)
                                                                   tcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACg                                                                         >  2:168696/1‑148 (MQ=255)
                                                                          gATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGaa                                                                   >  8:102677/1‑147 (MQ=255)
                                                                              cGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCgg                                                             <  7:173581/149‑1 (MQ=255)
                                                                              cGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCgg                                                             <  4:288648/149‑1 (MQ=255)
                                                                               ggAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCggg                                                            >  4:164387/1‑149 (MQ=255)
                                                                                 aaGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTc                                                          <  6:77178/149‑1 (MQ=255)
                                                                                   ggTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCAt                                                        >  3:111436/1‑149 (MQ=255)
                                                                                       ttgttgGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTg                                                      >  4:133329/1‑147 (MQ=255)
                                                                                          ttgGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGt                                                 >  1:131876/1‑149 (MQ=255)
                                                                                                  ctgcGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCAc                                                                      >  6:204291/1‑120 (MQ=255)
                                                                                                  ctgcGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCAc                                                                      <  5:204291/120‑1 (MQ=255)
                                                                                                            aaCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAg                               >  5:251881/1‑149 (MQ=255)
                                                                                                             aCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAgg                              <  7:102677/149‑1 (MQ=255)
                                                                                                             aCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAgg                              <  4:82203/149‑1 (MQ=255)
                                                                                                               gTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGgcgc                                    <  7:176773/141‑1 (MQ=255)
                                                                                                               gTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGgcgc                                    >  8:176773/1‑141 (MQ=255)
                                                                                                                tGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCa                           >  3:266175/1‑149 (MQ=255)
                                                                                                                   ggCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTg                        <  2:285370/149‑1 (MQ=255)
                                                                                                                     cTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGa                      <  2:131876/149‑1 (MQ=255)
                                                                                                                        tCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGac                     <  2:13651/147‑1 (MQ=255)
                                                                                                                          cTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTg                 >  4:177740/1‑149 (MQ=255)
                                                                                                                           tGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGc                >  6:222081/1‑149 (MQ=255)
                                                                                                                                cGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCt           >  6:143419/1‑149 (MQ=255)
                                                                                                                                 gATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTg          <  4:266175/149‑1 (MQ=255)
                                                                                                                                  ctgtgCGCAAC‑GCGCTGGCGCTGTTGCGGACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTgg         >  5:301037/2‑149 (MQ=255)
                                                                                                                                  aTGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTgg         >  8:181732/1‑149 (MQ=255)
                                                                                                                                   tgtgCGCAAC‑GCGCTGGCGCTGTTGCGGACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTggg        >  4:248116/1‑149 (MQ=255)
                                                                                                                                     tCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGt       >  6:200063/1‑148 (MQ=255)
                                                                                                                                       cGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAAt    <  5:267087/149‑1 (MQ=255)
                                                                                                                                       cGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAAt    >  1:174441/1‑149 (MQ=255)
                                                                                                                                         cAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGc  <  1:168696/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
ACTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGC  >  NC_002947/2783046‑2783331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑