Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,783,193 A→G 8.5% L77P (CTG→CCG) ‡ PP_2436 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,783,1930AG 8.5% 124.9 / 3.0 47L77P (CTG→CCG) ‡PP_2436LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base G (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

ACTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGCCGACGATCTG  >  NC_002947/2783046‑2783341
                                                                                                                                                   |                                                                                                                                                     
aCTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAcagcgccag                                                                                                                                                      >  4:293787/1‑149 (MQ=255)
  tGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAcagcgccagcg                                                                                                                                                    >  3:82203/1‑149 (MQ=255)
      ggCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑Gtt                                                                                                                                               <  3:293787/149‑1 (MQ=255)
         gcCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGACAGCGC‑GTTgcg                                                                                                                                            >  8:133543/1‑149 (MQ=255)
         gcCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAAC‑GCGCTGGCGCTGTTgcg                                                                                                                                            <  4:45612/149‑1 (MQ=255)
               cggcgCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTGCGCAAC‑GCGCTGGCGCTGTTGCGgacatc                                                                                                                                      <  4:33605/149‑3 (MQ=255)
                     aGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCgat                                                                                                                                <  2:217486/149‑1 (MQ=255)
                         gcTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGatgatg                                                                                                                            >  1:159325/1‑149 (MQ=255)
                                           aGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGtgtg                                                                                                          >  1:39814/1‑149 (MQ=255)
                                            gATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTgg                                                                                                         <  2:39814/149‑1 (MQ=255)
                                             aTGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTggg                                                                                                        <  4:297666/149‑1 (MQ=255)
                                                aCCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCAc                                                                                                     <  2:159325/149‑1 (MQ=255)
                                                       cGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCt                                                                                              >  1:285370/1‑149 (MQ=255)
                                                         accgaggcggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCtt                                                                                             <  1:328104/141‑1 (MQ=255)
                                                           cAGGTCGGTCATACTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTgcg                                                                                          >  5:77178/1‑149 (MQ=255)
                                                               tcggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGt                                                                                      <  5:64558/149‑1 (MQ=255)
                                                                cggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTa                                                                                     >  1:13651/1‑149 (MQ=255)
                                                                 ggtcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTa                                                                                     <  6:216252/148‑1 (MQ=255)
                                                                   tcATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACg                                                                                   >  2:168696/1‑148 (MQ=255)
                                                                          gATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGaa                                                                             >  8:102677/1‑147 (MQ=255)
                                                                              cGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCgg                                                                       <  7:173581/149‑1 (MQ=255)
                                                                              cGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCgg                                                                       <  4:288648/149‑1 (MQ=255)
                                                                               ggAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCggg                                                                      >  4:164387/1‑149 (MQ=255)
                                                                                 aaGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTc                                                                    <  6:77178/149‑1 (MQ=255)
                                                                                   ggTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCAt                                                                  >  3:111436/1‑149 (MQ=255)
                                                                                       ttgttgGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTg                                                                >  4:133329/1‑147 (MQ=255)
                                                                                          ttgGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGt                                                           >  1:131876/1‑149 (MQ=255)
                                                                                                  ctgcGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCAc                                                                                >  6:204291/1‑120 (MQ=255)
                                                                                                  ctgcGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCAc                                                                                <  5:204291/120‑1 (MQ=255)
                                                                                                            aaCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAg                                         >  5:251881/1‑149 (MQ=255)
                                                                                                             aCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAgg                                        <  7:102677/149‑1 (MQ=255)
                                                                                                             aCGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAgg                                        <  4:82203/149‑1 (MQ=255)
                                                                                                               gTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGgcgc                                              <  7:176773/141‑1 (MQ=255)
                                                                                                               gTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGgcgc                                              >  8:176773/1‑141 (MQ=255)
                                                                                                                tGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCa                                     >  3:266175/1‑149 (MQ=255)
                                                                                                                   ggCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTg                                  <  2:285370/149‑1 (MQ=255)
                                                                                                                     cTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGa                                <  2:131876/149‑1 (MQ=255)
                                                                                                                        tCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGac                               <  2:13651/147‑1 (MQ=255)
                                                                                                                          cTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTg                           >  4:177740/1‑149 (MQ=255)
                                                                                                                           tGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGc                          >  6:222081/1‑149 (MQ=255)
                                                                                                                                cGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCt                     >  6:143419/1‑149 (MQ=255)
                                                                                                                                 gATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTg                    <  4:266175/149‑1 (MQ=255)
                                                                                                                                  ctgtgCGCAAC‑GCGCTGGCGCTGTTGCGGACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTgg                   >  5:301037/2‑149 (MQ=255)
                                                                                                                                  aTGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTgg                   >  8:181732/1‑149 (MQ=255)
                                                                                                                                   tgtgCGCAAC‑GCGCTGGCGCTGTTGCGGACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTggg                  >  4:248116/1‑149 (MQ=255)
                                                                                                                                     tCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGt                 >  6:200063/1‑148 (MQ=255)
                                                                                                                                       cGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAAt              >  1:174441/1‑149 (MQ=255)
                                                                                                                                       cGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAAt              <  5:267087/149‑1 (MQ=255)
                                                                                                                                         cAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGc            <  1:168696/149‑1 (MQ=255)
                                                                                                                                                   agccc‑GTTTCCCACAGGCCCGAGCATGTTCTCGGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGAGGGTCATAGAAGGATTCGCGCAGGCGCGACAGTGCATTCGACACTGCCGGCTGGGTAATGCCGACGATCTg  <  6:70417/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACTGCCGGCGCCAGGCGGCGCAGGCGCTGGAACAGCGGCGGCAAGATGACCGCCTCGGTCAGGTCGGTCATGCTGATGCGGAAGGTCTTGTTGGCCTGCTGCGGGTTGAACGTGCGGCTTTCCTGCACCGATGTCCGCAACAGCGCCAGCGC‑GTTGCGCACAGGGCCGATGATGTTCTGCGCCATGGGTGTGGGCACCATGCCTTGCGCGGTACGCACGAACAGCGGGTCATTGAAGGTTTCGCGCAGGCGCGACAGGGCATTGGACACTGCCGGCTGGGTAATGCCGACGATCTG  >  NC_002947/2783046‑2783341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑