Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,799,186	C→T	7.4%	intergenic (‑200/+16)	PP_2452 ← / ← ansB	hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,799,186	0	C	T	7.4%	191.7 / 2.0	67	intergenic (‑200/+16)	PP_2452/ansB	hypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base T (2/3);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGC  >  NC_002947/2799041‑2799331                                                                                                                                                  |                                                                                                                                                   tCGCAACAGCTTGTCAGGAGAAATCCACCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAACCGATGTAGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACTAGGCCCTCCGAGCCGGCGCACCGGCCCTg                                                                                                                                                 >  3:230385/1‑149 (MQ=255)    cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTATACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGCCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                              <  1:326041/149‑1 (MQ=255)    cAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                              <  5:280512/149‑1 (MQ=255)     aaCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggg                                                                                                                                             <  8:47737/149‑1 (MQ=255)       cAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGcc                                                                                                                                                    <  8:68375/140‑1 (MQ=255)       cAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGcc                                                                                                                                                    >  7:68375/1‑140 (MQ=255)         gCTTGTCCCGAGAAATCCAGCGTTGGCCTGTAAACCATGACGCCGGTCAGAAAGCTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACCCGGTTGAGGAATTCGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGaa                                                                                                                                         <  4:92832/149‑1 (MQ=255)          cTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAg                                                                                                                                        >  8:167225/1‑149 (MQ=255)            tGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCa                                                                                                                                      >  3:57528/1‑149 (MQ=255)                aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCa                                                                                                                                 <  8:88751/149‑1 (MQ=255)                aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCa                                                                                                                                 <  7:295398/149‑1 (MQ=255)                 ggAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCAGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCGTGAGGGGGAAGCCC‑TCAg                                                                                                                                >  6:262874/1‑149 (MQ=255)                  gagaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAg                                                                                                                                >  4:182548/1‑148 (MQ=255)                    gaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGCTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTAc                                                                                                                             >  7:222739/1‑149 (MQ=255)                      aaTCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTc                                                                                                                           >  1:197866/1‑149 (MQ=255)                        tCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGTGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTccc                                                                                                                         <  6:162146/149‑1 (MQ=255)                                   ccTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGc                                                                                                              >  7:139534/1‑149 (MQ=255)                                     tGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTg                                                                                                            >  8:194514/1‑149 (MQ=255)                                         aaCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAg                                                                                                         <  2:197866/148‑1 (MQ=255)                                                aGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                                  <  8:139534/148‑1 (MQ=255)                                                    cGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGCTcag                                                                                                               >  2:112372/1‑129 (MQ=255)                                                    cGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTc                                                                                             >  2:313235/1‑149 (MQ=255)                                                       tCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAg                                                                                                         >  1:43891/1‑134 (MQ=255)                                                       tCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAg                                                                                                         <  2:43891/134‑1 (MQ=255)                                                        taGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                         <  5:307927/148‑1 (MQ=255)                                                         aGAAACGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                         <  5:71142/148‑1 (MQ=255)                                                          gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                         >  6:307927/1‑147 (MQ=255)                                                          gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGt                                                                                       >  4:307560/1‑149 (MQ=255)                                                              ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                   <  2:149934/149‑1 (MQ=255)                                                              ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                   <  6:136437/149‑1 (MQ=255)                                                              ggTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                   <  7:140878/149‑1 (MQ=255)                                                                tAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                                  <  7:65645/148‑1 (MQ=255)                                                                 aaaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                                 >  6:161661/1‑148 (MQ=255)                                                                  aaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTg                                                                                          >  5:162938/1‑138 (MQ=255)                                                                  aaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTg                                                                                          <  6:162938/138‑1 (MQ=255)                                                                     gATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCg                                                                            >  1:51843/1‑149 (MQ=255)                                                                      aTGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGc                                                                           <  1:18656/149‑1 (MQ=255)                                                                         tCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTa                                                                         <  7:167225/148‑1 (MQ=255)                                                                                 ttGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGc                                                                  >  2:117181/1‑147 (MQ=255)                                                                                         tctGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCcgc                                                        >  6:6128/1‑149 (MQ=255)                                                                                             tgtCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCtt                                                    >  7:135189/1‑149 (MQ=255)                                                                                              gtCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCttt                                                   >  7:260040/1‑149 (MQ=255)                                                                                                 tACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTCCCATCGCCAGGATCCGCGCTTTTTg                                                >  2:72082/1‑149 (MQ=255)                                                                                                 tACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATcc                                                          >  4:236995/1‑139 (MQ=255)                                                                                                 tACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATcc                                                          <  3:236995/139‑1 (MQ=255)                                                                                                  aCGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACAGGACCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATACGCGCTTTTTGc                                               >  1:278166/1‑149 (MQ=255)                                                                                                   cgcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                               <  1:117181/148‑1 (MQ=255)                                                                                                    gctgTTGAGGAATTGGCAACGAGGCCCTCTGAGCCGGCGCACCGTCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGTGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                             <  2:278166/146‑1 (MQ=255)                                                                                                    gcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                             <  1:313235/149‑1 (MQ=255)                                                                                                          gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCa                                        <  8:65520/148‑1 (MQ=255)                                                                                                             gAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTc                                    >  5:265228/1‑149 (MQ=255)                                                                                                             gAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTc                                    <  3:307560/149‑1 (MQ=255)                                                                                                               aTTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                                  <  5:161661/149‑1 (MQ=255)                                                                                                               aTTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                                  <  2:51843/149‑1 (MQ=255)                                                                                                                   gCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGc                              >  1:134805/1‑149 (MQ=255)                                                                                                                    cAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCTCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGcc                             <  8:222739/149‑1 (MQ=255)                                                                                                                          ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                <  6:197725/140‑1 (MQ=255)                                                                                                                          ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                >  5:197725/1‑140 (MQ=255)                                                                                                                          ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                       <  5:126844/149‑1 (MQ=255)                                                                                                                            cccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAcc                       >  3:45292/1‑147 (MQ=255)                                                                                                                             ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg                    >  8:215894/1‑149 (MQ=255)                                                                                                                              cTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg                    >  1:217856/1‑149 (MQ=255)                                                                                                                                 aggtccGGCGCACCGGCGCTGAGGGGGAAGCAC‑TCAGTACTCCCAGATGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCTGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAGCCAGTc                  <  4:230385/143‑1 (MQ=255)                                                                                                                                 agggCCGGTGCGCCGGCTCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGt                >  3:309335/4‑149 (MQ=255)                                                                                                                                      cGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTg           <  5:6128/149‑1 (MQ=255)                                                                                                                                      cGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTg           <  4:57528/149‑1 (MQ=255)                                                                                                                                      cGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTg           <  2:217856/149‑1 (MQ=255)                                                                                                                                             ccGGCTCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCg    <  4:45292/149‑1 (MQ=255)                                                                                                                                               ggCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGGCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGc  <  5:262874/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCAC‑TCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGC  >  NC_002947/2799041‑2799331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑