Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,862,973 A→T 9.7% Q11H (CAA→CAT)  gllR → HTH‑type transcriptional regulator GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,862,9730AT 9.7% 113.1 / 3.2 41Q11H (CAA→CAT) gllRHTH‑type transcriptional regulator GalR
Reads supporting (aligned to +/- strand):  ref base A (20/17);  new base T (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CCTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCATGC  >  NC_002947/2862827‑2863113
                                                                                                                                                  |                                                                                                                                            
ccTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGt                                                                                                                                            >  5:192196/1‑149 (MQ=255)
 cTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTc                                                                                                                                           >  6:24535/1‑149 (MQ=255)
      cAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCcgcgc                                                                                                                                      <  4:151920/149‑1 (MQ=255)
       aGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCcgcgc                                                                                                                                      <  4:156880/148‑1 (MQ=255)
            aCTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCAt                                                                                                                                >  2:257223/1‑149 (MQ=255)
              tAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATcc                                                                                                                              >  4:82638/1‑149 (MQ=255)
                    gTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCATGTCCGCGCGTTCATCCGGGTAg                                                                                                                        >  1:51349/1‑149 (MQ=255)
                    gTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGACCAACCTGATGCATGTCCGCGCGTTCATCCGGGTAg                                                                                                                        >  1:46672/1‑149 (MQ=255)
                       cATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGcc                                                                                                                      <  1:290/148‑1 (MQ=255)
                             cAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTg                                                                                                                <  6:194199/148‑1 (MQ=255)
                                cGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCa                                                                                                            <  6:192196/149‑1 (MQ=255)
                                             tatttattGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTTCCCAACCTGTTGCAATTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTcgcgtg                                                                                                <  2:77171/148‑3 (MQ=255)
                                                  attGCCGGGCAAGCGGGGTAGAGCCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACg                                                                                           <  6:38412/148‑1 (MQ=255)
                                                     gCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAg                                                                                        <  3:82638/148‑1 (MQ=255)
                                                       cGGGCAAGCGGGGTCGAGGCATAGGCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTg                                                                                     <  3:182974/149‑1 (MQ=255)
                                                        gggCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGc                                                                                    >  2:119700/1‑149 (MQ=255)
                                                            aaGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGtt                                                                                >  5:13633/1‑149 (MQ=255)
                                                              gCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTcc                                                                              <  5:24535/149‑1 (MQ=255)
                                                               cGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCg                                                                             <  1:119700/149‑1 (MQ=255)
                                                                   gTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCt                                                                         >  6:270238/1‑149 (MQ=255)
                                                                           aTAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTg                                                                           <  7:190707/139‑1 (MQ=255)
                                                                           aTAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTg                                                                           >  8:190707/1‑139 (MQ=255)
                                                                                                               cTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCtt                             >  5:26461/1‑149 (MQ=255)
                                                                                                                ttATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCttt                            >  4:577/1‑149 (MQ=255)
                                                                                                                  aTGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCcgc                                                       <  7:283130/120‑1 (MQ=255)
                                                                                                                  aTGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCcgc                                                       >  8:283130/1‑120 (MQ=255)
                                                                                                                    gATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCg                        >  1:227057/1‑149 (MQ=255)
                                                                                                                       cGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGc                     >  5:150353/1‑149 (MQ=255)
                                                                                                                        gATGTCGAACTGCCCAACCTGATGCATGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGcc                    <  2:51349/149‑1 (MQ=255)
                                                                                                                        gATGTCGAACTGCCCAACCTGATGCATGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGcc                    <  2:46672/149‑1 (MQ=255)
                                                                                                                        gATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGAGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGc                     <  6:13633/148‑1 (MQ=255)
                                                                                                                          tGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGcccc                  >  2:92691/1‑149 (MQ=255)
                                                                                                                            tCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTg                >  3:100973/1‑149 (MQ=255)
                                                                                                                              gAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGt               <  5:270238/148‑1 (MQ=255)
                                                                                                                               aaCTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTc             >  6:38924/1‑149 (MQ=255)
                                                                                                                                    cccAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTCGCCGTGCCCCTGTTCGAgcg        >  8:132286/1‑149 (MQ=255)
                                                                                                                                      cAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAgcgc       >  3:13583/1‑148 (MQ=255)
                                                                                                                                      cAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAgcgc       >  6:301605/1‑148 (MQ=255)
                                                                                                                                        aCCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCAt    <  6:275342/149‑1 (MQ=255)
                                                                                                                                          cTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCATGc  >  1:169458/1‑149 (MQ=255)
                                                                                                                                          cTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTCGCCGTGCCCCTGTTCGAGCGCCATGc  <  7:132286/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
CCTGTGCAGGGCACTAATGCGTTCATTTGCAACGGCTATGCGTTTTATTTATTGCCGGGCAAGCGGGGTAGAGGCATAGTCGCCGCAGTCACGAGCCGAGCGCCGGGAGCCCTTATGATCGATGTCGAACTGCCCAACCTGATGCAAGTCCGCGCGTTCATCCGGGTAGCCGAGCTGGGCAGCGTTTCGCGGGCGACGGAGGTGCTGTTCCGTGCTCAGTCCGTAGTCACCCGCGCCATCGCCGAATTGGAGGCGCGCTTTGCCGTGCCCCTGTTCGAGCGCCATGC  >  NC_002947/2862827‑2863113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑