Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,921,460 G→A 100% G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA100.0% 121.3 / ‑6.8 38G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/16);  minor base C (1/0);  total (22/16)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921323‑2921602
                                                                                                                                          |                                                                                                                                              
tGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCCGGGGGGCCgg                                                                                                                                      >  4:146780/1‑148 (MQ=255)
          cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGAAGGGCGGCGATGGTAACGACACATTGTTCGGAAACGTGGGTACCGA‑CACCCTGATCAGGGGGGACGGCAACGACTTca                                                                                                                           >  5:323072/1‑148 (MQ=255)
           ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACCCCCCTGATCagg                                                                                                                                              <  4:40144/130‑1 (MQ=255)
           ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACCCCCCTGATCagg                                                                                                                                              >  3:40144/1‑130 (MQ=255)
                 gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                    >  7:243174/1‑149 (MQ=255)
                   cAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTg                                                                                                                  >  4:177343/1‑149 (MQ=255)
                    aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                 >  1:278489/1‑149 (MQ=255)
                    aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                 <  8:4083/149‑1 (MQ=255)
                    aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                 >  7:147702/1‑149 (MQ=255)
                                   gcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTcc                                                                                                                           >  6:314298/1‑124 (MQ=255)
                                           tGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGtggtgg                                                                                          >  8:96665/1‑149 (MQ=255)
                                                  cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGc                                                                                   <  7:96665/149‑1 (MQ=255)
                                                     taTGGCTGCAACGTCAATGACACCCTGCAGGGAGGAGATGTTAAAGACAAATTGATCGGCAACGTTGGTACCGA‑CACCATGATCATGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGTTAACGATACGGTGGTGGTTGGGGCCg                                                                                 <  6:323072/147‑1 (MQ=255)
                                                          cggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACg                                                                           <  2:278489/149‑1 (MQ=255)
                                                           ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGCTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa                                                                          >  8:156939/1‑149 (MQ=255)
                                                                 ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                    >  2:290001/1‑149 (MQ=255)
                                                                   caaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCagg                                                                                                                                              >  2:316648/1‑73 (MQ=255)
                                                                   caaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCagg                                                                                                                                              <  1:316648/73‑1 (MQ=255)
                                                                     aTGACACCCTGAAGGGCGGCTATGTTAACTACAAATTGCTCGGCGACGTGGGTACCTA‑CACACTGATCAGGGGGGCCGGCAACTACTTCCTCAACGGTTCGGCGGGTAACGATACGGTGGTGGGTGTGGCCGGTAACGACACCATGATc                                                                <  8:147865/149‑1 (MQ=255)
                                                                          acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTaa                                                                                                        >  6:159062/1‑104 (MQ=255)
                                                                          acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTaa                                                                                                        <  5:159062/104‑1 (MQ=255)
                                                                            ggtGCAGGGCTGCGATTGTAAGGACACATTGTTCGTCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGGATGTTTTTACTCAAGGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                       <  2:242774/133‑1 (MQ=255)
                                                                            ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                       >  1:242774/1‑135 (MQ=255)
                                                                               gCAGGGCGGCGATGGTAACGACACCTTGTGCGGCGACGTGGGTACCGA‑CACCCTGAGCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATg                                                      <  1:167936/149‑1 (MQ=255)
                                                                                     cggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACg                                                <  1:290001/149‑1 (MQ=255)
                                                                                       gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGGGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                              <  8:243174/149‑1 (MQ=255)
                                                                                           tGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATgtgt                                          <  7:156939/149‑1 (MQ=255)
                                                                                             gTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc                                        >  8:276805/1‑149 (MQ=255)
                                                                                               aaCGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAACCGATGTGTTc                                        >  6:197156/1‑147 (MQ=255)
                                                                                                       aTTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTGACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                              >  2:85244/1‑149 (MQ=255)
                                                                                                          gTTCGGCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCa                            <  7:276805/148‑1 (MQ=255)
                                                                                                              ggCGACGTGGGTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                       >  3:240085/1‑149 (MQ=255)
                                                                                                                      gggTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc                >  4:287219/1‑148 (MQ=255)
                                                                                                                       ggTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg                                 >  7:181254/1‑130 (MQ=255)
                                                                                                                       ggTACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg                                 <  8:181254/130‑1 (MQ=255)
                                                                                                                        gTACAGA‑CACCCTGATCAGGGGGGCCGGCACCGACTTCCTCAACGGTGCGGCTGGTAACGATACGGTGGTGGGTGGGGCCGGTACCGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTCCAGGCTTTGGCAACGAc             >  2:315921/1‑149 (MQ=255)
                                                                                                                         tACCGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc            <  8:147702/149‑1 (MQ=255)
                                                                                                                           ccGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCt           <  3:177343/148‑1 (MQ=255)
                                                                                                                            cGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                       >  8:163578/1‑135 (MQ=255)
                                                                                                                            cGA‑CACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt                       <  7:163578/135‑1 (MQ=255)
                                                                                                                                   ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc    >  3:167348/1‑148 (MQ=255)
                                                                                                                                    cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCaa  >  7:91146/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                              
TGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGA‑CACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921323‑2921602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑