| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 212,694 | T→A | 7.7% | L6067Q (CTG→CAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 212,694 | 0 | T | A | 7.7% | 137.3 / 3.0 | 52 | L6067Q (CTG→CAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (23/25); new base A (2/2); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01 | |||||||||||
GTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGG > NC_002947/212546‑212841 | gTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCt < 2:62794/149‑1 (MQ=34) ggctcGTCGGTAACCGACGAGCCAGGTAGGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGt > 1:185750/5‑149 (MQ=12) aGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg < 5:147053/148‑1 (MQ=34) gCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTc < 8:36896/149‑1 (MQ=34) gCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTc < 7:239185/149‑1 (MQ=34) gCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTc < 5:74013/149‑1 (MQ=34) cACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCg > 8:178671/1‑149 (MQ=34) aCGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGt > 3:210776/1‑149 (MQ=34) gTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggcca < 2:84183/149‑6 (MQ=19) aCCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTTCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 6:123068/148‑1 (MQ=17) aCCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 2:185750/148‑1 (MQ=32) aCCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 4:210776/148‑1 (MQ=32) ccGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc < 4:111713/149‑1 (MQ=32) cgacgaGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc > 6:94523/1‑148 (MQ=32) gCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa > 3:102044/1‑140 (MQ=32) gCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 4:102044/140‑1 (MQ=32) ccAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGc > 2:293932/1‑149 (MQ=32) aGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc > 2:212140/1‑148 (MQ=32) gTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg < 7:192092/149‑1 (MQ=32) cGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGt < 6:49769/148‑1 (MQ=32) gCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGGCCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCa > 1:231311/1‑149 (MQ=17) ccGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCAc < 3:23318/149‑1 (MQ=32) cGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCAc > 4:158044/1‑148 (MQ=32) gCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAt > 4:30830/1‑148 (MQ=32) cAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑Ca < 3:158044/149‑1 (MQ=32) aaCGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑c > 4:168254/1‑147 (MQ=32) gCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGc < 1:212140/149‑1 (MQ=32) ccTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGaaa < 4:135657/149‑1 (MQ=31) cGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCGGGGTGATGACGTc > 3:181627/1‑148 (MQ=11) cGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTc > 3:64449/1‑148 (MQ=11) gagaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTa > 4:89584/1‑149 (MQ=32) aCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAac < 4:64449/149‑1 (MQ=32) tCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAacgat < 3:269314/149‑2 (MQ=17) aGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCt < 3:96068/149‑1 (MQ=32) ccGTGCACGTCAACCAGCCGCTGGCCCACTACCTGGGCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGTCTTGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGc < 4:91018/149‑1 (MQ=255) cGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGcc > 5:158144/1‑149 (MQ=32) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑AAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTggg > 6:230209/1‑148 (MQ=17) gTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCGGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTAt < 4:181627/149‑1 (MQ=21) tCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATc < 3:89584/149‑1 (MQ=34) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCaa > 5:198944/1‑149 (MQ=35) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCaa < 5:194733/149‑1 (MQ=35) ccAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACt > 7:108885/1‑149 (MQ=37) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTcgg < 1:293932/149‑1 (MQ=37) gggtcACGACCAGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTg > 2:122470/5‑148 (MQ=255) gggtcACGACCAGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTg > 2:24525/5‑148 (MQ=255) gCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTg < 5:230209/147‑1 (MQ=37) gtcACGACCAGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGAc < 1:122470/147‑1 (MQ=37) ccACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACg > 4:47825/1‑149 (MQ=37) aCGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATGCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGc > 1:62217/1‑149 (MQ=255) gACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCAc > 6:15844/1‑149 (MQ=37) ccTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTg < 6:198944/149‑1 (MQ=37) ccTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTg > 4:243632/1‑149 (MQ=37) cTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTgg > 3:16547/1‑149 (MQ=37) | GTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAT‑CAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGACGCCACTGG > NC_002947/212546‑212841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |