Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,018,668 G→A 8.0% G102G (GGG→GGA)  bcsZ → cellulose synthase endo‑1,4‑D‑glucanase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,018,6680GA 8.0% 144.5 / 2.1 50G102G (GGG→GGA) bcsZcellulose synthase endo‑1,4‑D‑glucanase subunit
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCACCTGCTGTGGCGTATCGCCGCGCAGA  >  NC_002947/3018523‑3018816
                                                                                                                                                 |                                                                                                                                                    
cGGAAGGGCAGAGTTACGAGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGgcag                                                                                                                                                   >  7:157551/1‑149 (MQ=255)
   aaGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGgcagcag                                                                                                                                                <  8:157551/149‑1 (MQ=255)
     gggCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGgcagcag                                                                                                                                                >  7:114866/1‑147 (MQ=255)
       gCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGt                                                                                                                                            >  6:226771/1‑149 (MQ=255)
        cAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGTGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTg                                                                                                                                           <  8:286161/149‑1 (MQ=255)
        cAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTg                                                                                                                                           <  6:236721/149‑1 (MQ=255)
                  cgcTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACGTCGCCGAGGGTGACCTGGCCCGGCAGCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTg                                                                                                                                 <  1:104117/149‑1 (MQ=255)
                    cTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAg                                                                                                                                       <  1:110049/141‑1 (MQ=255)
                    cTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAg                                                                                                                                       >  2:110049/1‑141 (MQ=255)
                         cttcGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGcc                                                                                                                           >  8:259280/1‑148 (MQ=255)
                           tcGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaa                                                                                                                         >  6:312493/1‑148 (MQ=255)
                             gCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaacaa                                                                                                                      >  3:97880/1‑149 (MQ=255)
                                       gtaACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGAt                                                                                                            >  8:283021/1‑149 (MQ=255)
                                          aCGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGc                                                                                                          <  1:25683/148‑1 (MQ=255)
                                                ggCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCtg                                                                                                   <  8:114866/149‑1 (MQ=255)
                                                 gCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGgcagc                                                                                                                                                  >  6:271821/1‑101 (MQ=255)
                                                 gCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaac                                                                                                                        >  2:106213/1‑127 (MQ=255)
                                                 gCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCtgt                                                                                                  <  8:222723/149‑1 (MQ=255)
                                                 gCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGCGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaac                                                                                                                        <  1:106213/127‑1 (MQ=255)
                                                      ccTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATc                                                                                             <  4:97880/149‑1 (MQ=255)
                                                       cTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCg                                                                                                        <  6:325378/137‑1 (MQ=255)
                                                       cTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCg                                                                                                        >  5:325378/1‑137 (MQ=255)
                                                             gcAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTa                                                                                        >  1:178280/1‑147 (MQ=255)
                                                             gcAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACa                                                                                      >  4:214656/1‑149 (MQ=255)
                                                               aGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGc                                                                                    <  6:38048/149‑1 (MQ=255)
                                                                gTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGACAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGcc                                                                                   >  5:197801/1‑149 (MQ=255)
                                                                gTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGACAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGcc                                                                                   >  5:202332/1‑149 (MQ=255)
                                                                    ctgcGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCctgct                                                                               <  3:214656/149‑1 (MQ=255)
                                                                    ctgcGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGACAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCctgct                                                                               <  6:197801/149‑1 (MQ=255)
                                                                    ctgcGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGACAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCctgct                                                                               <  6:202332/149‑1 (MQ=255)
                                                                      gcGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCctgctg                                                                              >  4:268105/1‑148 (MQ=255)
                                                                       cGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaacaa                                                                                                                      <  5:269065/107‑1 (MQ=255)
                                                                       cGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCcaacaa                                                                                                                      >  6:269065/1‑107 (MQ=255)
                                                                              ccAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAggg                                                                     <  5:226771/149‑1 (MQ=255)
                                                                               cAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGc                                                                    >  1:288737/1‑149 (MQ=255)
                                                                                aGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCg                                                                   <  7:259280/149‑1 (MQ=255)
                                                                                 gCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCgg                                                                  >  1:4781/1‑149 (MQ=255)
                                                                                      aacGTCGCCGAGGGTGAACTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACCACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTtgtg                                                             <  7:283021/149‑1 (MQ=255)
                                                                                       acCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCAGCTGGAAGCAGGGCGGTTGTgg                                                            <  2:178280/149‑1 (MQ=255)
                                                                                        ccTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTtgtcg                                                            >  5:134977/1‑146 (MQ=255)
                                                                                                aGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCg                                                   <  8:153668/149‑1 (MQ=255)
                                                                                                  ggTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCCACAACGCCAGCGATGCAGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCgcc                                                 >  8:294013/1‑147 (MQ=255)
                                                                                                  ggTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGc                                                 <  2:4781/149‑1 (MQ=255)
                                                                                                   gTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGcc                                                <  7:185870/149‑1 (MQ=255)
                                                                                                        cTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTacac                                           >  4:121550/1‑149 (MQ=255)
                                                                                                          ggCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGc                                                                                           >  4:129829/1‑99 (MQ=255)
                                                                                                          ggCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGc                                                                                           <  3:129829/99‑1 (MQ=255)
                                                                                                             ccGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAAc                                      >  6:131174/1‑149 (MQ=255)
                                                                                                                gCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTgg                                   <  5:312493/149‑1 (MQ=255)
                                                                                                                              ggCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCACctgctg                     >  5:105590/1‑149 (MQ=255)
                                                                                                                                     gggCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCAGgtgctggcccagt              <  2:288737/149‑8 (MQ=255)
                                                                                                                                       gCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGCCGCCAACAACGCCAGCGATGACGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGTGCCAGAACCTGCTGTGTCGTATc            >  6:83237/1‑149 (MQ=255)
                                                                                                                                       gCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGc                                                                         <  6:59466/88‑1 (MQ=255)
                                                                                                                                       gCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGc                                                                         >  5:59466/1‑88 (MQ=255)
                                                                                                                                               gggCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCACCTGCTGTGGCGTATCGCcgcg      >  8:128807/1‑147 (MQ=255)
                                                                                                                                                 gcagcagcaGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCTATCTGTGGATCGCCTACATCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCACCTGCTGTAGCTTATCGCCGCGCAGa  >  4:170855/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGAAGGGCAGAGTTACGCGCTGTTCTTCGCCCTGGTAGGTAACGACCGGCAAACCTTCGCGCAGTTGCTGCGTTGGACCAGCAACAACCTCGCCGAGGGTGACCTGGCCCGGCATCTGCCTGCCTGGCTGTGGGGCCGTGACGGGCAGCAGCAGTGGCAGGTGCTGGACGCCAACAACGCCAGCGATGCCGATCTGTGGATCGCCTACAGCCTGCTGGAAGCAGGGCGGTTGTGGGACCAGCCGGCCTACACGCAACTGGGCCAGCACCTGCTGTGGCGTATCGCCGCGCAGA  >  NC_002947/3018523‑3018816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑