Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,029,214 G→C 12.3% L289F (TTG→TTC)  pcaY → aromatic acid chemoreceptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,029,2140GC12.3% 102.6 / 4.6 38L289F (TTG→TTC) pcaYaromatic acid chemoreceptor
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base C (2/2);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

CTGAGTGTGCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCAAACCACCAGT  >  NC_002947/3029071‑3029355
                                                                                                                                               |                                                                                                                                             
cTGAGTGTGCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGc                                                                                                                                          >  5:283082/1‑149 (MQ=255)
    gtgtGCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTCAACGCCGtg                                                                                                                                      >  6:181080/1‑148 (MQ=255)
    gtgtGACGGTGCAAGGGCAGGGTAACAACGAAAACGTCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTCTGCCCGGCAGCTGGACGCCGCCGCCACGGCGCTAAacgacgag                                                                                                                                      >  4:22566/1‑147 (MQ=255)
       tgCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTCAACGCCGtggcg                                                                                                                                   <  3:22566/149‑5 (MQ=255)
       tgCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTCAACGCCGtggcg                                                                                                                                   <  5:181080/149‑5 (MQ=255)
         ccGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCAcc                                                                                                                                   >  4:176610/1‑147 (MQ=255)
               cAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAg                                                                                                                           >  2:167521/1‑149 (MQ=255)
                aaGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAgc                                                                                                                          >  7:45665/1‑149 (MQ=255)
                  gggCAGTGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAgcgc                                                                                                                        <  8:109267/149‑1 (MQ=255)
                   ggCAGGGTAACAACGACATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGcc                                                                                                                       <  5:180454/149‑1 (MQ=255)
                     cAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCcaa                                                                                                                     >  5:93375/1‑149 (MQ=255)
                             caacGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGc                                                                                                             <  6:283082/149‑1 (MQ=255)
                              aacGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGcc                                                                                                                       >  1:18959/1‑138 (MQ=255)
                              aacGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGcc                                                                                                                       <  2:18959/138‑1 (MQ=255)
                                cGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGt                                                                                                           >  4:204731/1‑148 (MQ=255)
                                            aCTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAg                                                                                               >  6:209315/1‑148 (MQ=255)
                                                ttCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGc                                                                                           <  8:45665/148‑1 (MQ=255)
                                                ttCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCt                                                                                          <  3:176610/149‑1 (MQ=255)
                                                  ccATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCACCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTgg                                                                                        >  6:120450/1‑149 (MQ=255)
                                                        gCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAgg                                                                                  >  7:262962/1‑149 (MQ=255)
                                                         cTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGgc                                                                                 >  4:230759/1‑149 (MQ=255)
                                                                  aTGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCACCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGc                                                                        <  5:120450/149‑1 (MQ=255)
                                                                        cagAGCCAGCGCGACACTCTGGGCCAGATCAACAACTCTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGAc                                                                  <  4:255123/149‑1 (MQ=255)
                                                                             ccAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAgg                                                                                  >  6:290767/1‑128 (MQ=255)
                                                                             ccAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAgg                                                                                  <  5:290767/128‑1 (MQ=255)
                                                                              cAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAAt                                                            >  8:27229/1‑149 (MQ=255)
                                                                                      cacTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACgg                                                    <  6:93375/149‑1 (MQ=255)
                                                                                      cacTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACgg                                                    <  3:204731/149‑1 (MQ=255)
                                                                                         tctGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAg                                                 >  2:55751/1‑149 (MQ=255)
                                                                                                     caacaaCTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTg                                     >  3:25343/1‑149 (MQ=255)
                                                                                                                cccGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAAcgcg                          <  5:209315/149‑1 (MQ=255)
                                                                                                                 ccGGCAGCTTCACGCCGCCGCCACGGCGGTGAACGCCGTCACCGACGAAAGCCCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGa                         <  5:71588/149‑1 (MQ=255)
                                                                                                                         tGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGCCCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCaccacc                 >  5:322934/1‑149 (MQ=255)
                                                                                                                           gACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCGGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTc               >  6:70444/1‑149 (MQ=255)
                                                                                                                                cgccgccACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCaaa          <  4:25343/149‑1 (MQ=255)
                                                                                                                                  ccgccACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCAAacc        >  1:48843/1‑149 (MQ=255)
                                                                                                                                      cACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCAAAccacca    <  7:27229/149‑1 (MQ=255)
                                                                                                                                        cGGCGTTCAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCAAACCACCAGt  >  8:68915/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                             
CTGAGTGTGCCGGTGCAAGGGCAGGGTAACAACGAAATCGGCCAACTGTTCCATGAGCTCCAGCGCATGCAGCAGAGCCAGCGCGACACTCTGGGGCAGATCAACAACTGTGCCCGGCAGCTGGACGCCGCCGCCACGGCGTTGAACGCCGTCACCGAGGAAAGCGCCAACAACCTGCGTCAGCAGGGGCAGGAGCTGGAGCAGGCCGCCACTGCCGTGACCGAAATGACCACGGCAGTGGAGGAAGTTGCGCGCAACGCGATCACCACCTCGCAAACCACCAGT  >  NC_002947/3029071‑3029355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑