| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,120,936 | T→A | 10.9% | I170F (ATC→TTC) | PP_2737 ← | short‑chain dehydrogenase/reductase family oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,120,936 | 0 | T | A | 10.9% | 120.0 / 6.4 | 46 | I170F (ATC→TTC) | PP_2737 | short‑chain dehydrogenase/reductase family oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (21/20); new base A (3/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
GTTGATTTCCAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTGCGCGA > NC_002947/3120793‑3121081 | gTTGATTTCCAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCtt > 8:216777/1‑149 (MQ=255) gATTTCCGGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTcgc > 3:116859/1‑149 (MQ=255) ccAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGc > 2:248406/1‑148 (MQ=255) ccAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGAAGGCATCGCggtc < 5:200431/148‑3 (MQ=255) cAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGc > 7:12932/1‑147 (MQ=255) ggggCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑Gt < 6:151212/149‑1 (MQ=255) gcCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACC‑CAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGTGATGCCTTCGCGGGCCAG‑GT‑CGATTCg < 1:9280/149‑1 (MQ=255) ccGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCg > 3:191226/1‑149 (MQ=255) gggCAGGCGCCTGGCGATATGCCGGGCTGCACGCTGCTCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CTATTCGCt < 8:68921/149‑1 (MQ=255) gggCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCt < 7:12093/149‑1 (MQ=255) gCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCtgt < 2:169/149‑1 (MQ=255) gCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCtgt < 5:257847/149‑1 (MQ=255) gCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCtgt < 7:216777/149‑1 (MQ=255) tGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTccac > 6:19856/1‑148 (MQ=255) tGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAgcg < 6:74490/149‑1 (MQ=255) cTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTgg < 4:191226/149‑1 (MQ=255) cacaGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCa > 5:25783/1‑147 (MQ=255) cacaGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCa > 7:205768/1‑147 (MQ=255) aGCTGTGGCATGGGGAACTCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGg‑c < 8:59789/149‑1 (MQ=255) cTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGg‑cgc < 5:19856/149‑1 (MQ=255) gCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTccacc < 5:84374/107‑1 (MQ=255) gCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTccacc > 6:84374/1‑107 (MQ=255) cGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGgccagcc < 6:328839/148‑1 (MQ=255) aCGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGccagccag < 3:19758/148‑1 (MQ=255) aCGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGccagccag > 4:19758/1‑148 (MQ=255) gCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTg > 6:143793/1‑149 (MQ=255) cACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATca > 2:100576/1‑149 (MQ=255) aCGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GTACGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATca > 1:240521/1‑149 (MQ=255) aCGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATcac > 5:283229/1‑149 (MQ=255) gAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATcacca < 2:240521/149‑1 (MQ=255) ggCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGGCCGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAgg < 1:248406/147‑1 (MQ=255) gCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTggg > 1:322028/1‑149 (MQ=255) ccAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGt > 1:167623/1‑149 (MQ=255) ccAGGGTGACCGCGATGCCATCGCGGGCAAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCAAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGt > 2:24052/1‑149 (MQ=255) cAGGGTGACCGCGATGCCTTCGCGGGCCAGTGT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGt > 1:194810/1‑149 (MQ=255) acctggcccGCGAAGGCATCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGttt > 8:209649/8‑149 (MQ=255) aGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGttt < 8:205768/149‑1 (MQ=255) ctggcccGCGAAGGCATCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGc > 3:112637/6‑149 (MQ=255) tGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCt > 3:166519/1‑149 (MQ=255) gcccGCGAAGGCATCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTg > 7:66879/3‑149 (MQ=255) cccGCGAAGGCATCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCttc < 7:209649/148‑3 (MQ=255) cgcgATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGc > 2:160592/1‑143 (MQ=255) cgcgATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGc < 1:160592/143‑1 (MQ=255) cgcgATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTgcgc > 8:124954/1‑149 (MQ=255) cgATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACGAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTGCGCGa < 6:283229/149‑1 (MQ=255) cgATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTGCGCGa < 4:166519/149‑1 (MQ=255) | GTTGATTTCCAGGGGGCGGCCGGGCAGGCGCCTGGCGATATGCCGGGCGGCACGCTGCGCAGACCACAGCTGTGGCATGGGGAAATCGTTGCGACGGGTCAGGGGCGTGTCCACGAAGCCTGGGCTCACCAGGGTGACCGCGATGCCTTCGCGGGCCAG‑GT‑CGATTCGCTGTGACTCCACCAGATAGCGCACTGCGGCCTTGGACGCCCCATAGG‑CGCCAGCGCGGGGCAAGGCCAGCCAGGTTACCGAACTGCCCATCACCACCAGGTGGGGGCGTTTGCCTGCGCGA > NC_002947/3120793‑3121081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |