Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,162,524 T→C 8.7% intergenic (+471/‑109) PP_2776 → / → PP_2777 homocysteine S‑methyltransferase family protein/acyl carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,162,5240TC 8.7% 124.2 / 2.3 46intergenic (+471/‑109)PP_2776/PP_2777homocysteine S‑methyltransferase family protein/acyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base C (2/2);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTTCTGGAACTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCAT  >  NC_002947/3162377‑3162664
                                                                                                                                                     |                                                                                                                                            
cGCTTCTGGAACTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGAGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCt                                                                                                                                              >  5:158623/1‑148 (MQ=255)
       ggAACTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTAAGAACCCTCTGCAAGCCCCATTTAGCTGCATTAATCAAAGTAGTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAggg                                                                                                                                         <  1:261186/148‑1 (MQ=255)
        gAACTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAgggg                                                                                                                                        <  6:96279/146‑1 (MQ=255)
         aaCTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGgaga                                                                                                                                    <  7:206054/149‑1 (MQ=255)
            tATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGTGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCCTAAGGGgaga                                                                                                                                    <  7:117706/146‑1 (MQ=255)
            tATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCCTAAGGGgaga                                                                                                                                    >  8:117706/1‑146 (MQ=255)
            tATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTCACTCTCCGATTAGGGGGAGCCtt                                                                                                                                 >  8:249269/1‑149 (MQ=255)
              tCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACtt                                                                                                                                 <  1:51660/147‑1 (MQ=255)
              tCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTa                                                                                                                               >  7:228675/1‑149 (MQ=255)
                   cTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGAGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGCCTCGACCCTTAGGGGGTCGCTTTAGACTc                                                                                                                          >  2:295344/1‑149 (MQ=255)
                    tGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTcc                                                                                                                         >  3:217676/1‑149 (MQ=255)
                      tttCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGTCTAAAGTCTccc                                                                                                                        <  4:179700/148‑1 (MQ=255)
                      tttCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACtt                                                                                                                                 <  7:173707/139‑1 (MQ=255)
                      tttCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACtt                                                                                                                                 >  8:173707/1‑139 (MQ=255)
                        tCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACttt                                                                                                                                >  2:253272/1‑138 (MQ=255)
                        tCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGCATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACttt                                                                                                                                <  1:253272/138‑1 (MQ=255)
                         cAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGTCTAAAGTCTccccct                                                                                                                     <  5:245135/148‑4 (MQ=255)
                         cAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCACTTAGGGGGAGACTTTAGACTCCCGGcc                                                                                                                    >  4:87851/1‑149 (MQ=255)
                               aTCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGa                                                                                                               >  6:111861/1‑148 (MQ=255)
                                tCGATTTTGTCATTTTACGACCCCTCTCCAAGACCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGa                                                                                                               >  6:151811/1‑147 (MQ=255)
                                       tGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTg                                                                                                       <  3:29568/148‑1 (MQ=255)
                                                    ccccTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCt                                                                                         >  2:182329/1‑149 (MQ=255)
                                                     cccTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCtt                                                                                        >  8:2173/1‑149 (MQ=255)
                                                            cAAGCCCCATTGAGCTGCATTAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTgg                                                                                 <  7:118796/149‑1 (MQ=255)
                                                                ccccATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCt                                                                                                        >  4:160962/1‑122 (MQ=255)
                                                                ccccATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCt                                                                                                        <  3:160962/122‑1 (MQ=255)
                                                                         gCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGTGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTTGCCTGAAAACAGCTTTTTTTGGCCCGATGACTTTa                                                                    <  1:295344/149‑1 (MQ=255)
                                                                          cTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTat                                                                   <  5:111861/149‑1 (MQ=255)
                                                                            gCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGAGTTTattc                                                                 >  2:238557/1‑149 (MQ=255)
                                                                               tGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCAGGCACCCGATAGATATGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTattcatt                                                              >  2:40195/1‑149 (MQ=255)
                                                                                             ttCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCttt                                                  >  1:153246/1‑149 (MQ=255)
                                                                                              tCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCttt                                                  <  8:228675/148‑1 (MQ=255)
                                                                                                aGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCTGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGAAGACTTTATTCATTCAATGCGGCTTTTGt                                               <  1:182329/149‑1 (MQ=255)
                                                                                                 gggCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTa                                              <  6:158623/149‑1 (MQ=255)
                                                                                                   gggcgcTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAg                                             <  7:249269/146‑1 (MQ=255)
                                                                                                                    acaAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGaat                            >  3:210388/1‑148 (MQ=255)
                                                                                                                       aaaaGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATc                        <  5:151811/149‑1 (MQ=255)
                                                                                                                            cACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTg                   <  3:87851/149‑1 (MQ=255)
                                                                                                                                  tGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAAtt              <  7:10850/148‑1 (MQ=255)
                                                                                                                                    gCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAgg                                            <  7:116268/116‑1 (MQ=255)
                                                                                                                                    gCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAgg                                            >  8:116268/1‑116 (MQ=255)
                                                                                                                                     cccGTTGCCTCGCCGCTGAGGGGGAGACTTTAGACTCCCGGCCACCTATAGATCTGGCCTCAAAACATCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGAc          <  4:217676/147‑1 (MQ=255)
                                                                                                                                        gTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGc       <  7:2173/149‑1 (MQ=255)
                                                                                                                                         taaagtctcCCCCTAAGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAacac                                     <  6:115606/113‑1 (MQ=255)
                                                                                                                                         taaagtctcCCCCTAAGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAacac                                     >  5:115606/6‑118 (MQ=255)
                                                                                                                                              tctcCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGtcat  >  5:207852/1‑148 (MQ=255)
                                                                                                                                              tctcCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTGTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTGGGGAAACACTCAATGAATAATCCGTTGGAAGTGGAGAGGGtcat  >  4:55070/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
CGCTTCTGGAACTATCGTGCTGTTTCAAGCAATCGATTTTGTCATTTTACGACCCCTCTCCAAGCCCCATTGAGCTGCATGAATCAAAG‑‑GTTTCAGGGCGCGCTGGGATTTCTCACAAAAAGCACTGATGGCACGTTGACTCTCCCCTTAGGGGGAGACTTTAGACTCCCGGCCCCCGATAGATCTGGCCTCAAAACAGCTTTTTTTGGCCCGATGACTTTATTCATTCAATGCGGCTTTTGTAGGGAAACACTCAATGAATAATCCGTTGGAATTGGACAGCGTCAT  >  NC_002947/3162377‑3162664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑