Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,189,278 G→C 10.0% L458V (CTG→GTG)  PP_2799 ← class III aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,189,2780GC10.0% 115.2 / 4.8 40L458V (CTG→GTG) PP_2799class III aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (20/16);  new base C (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

ATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGC  >  NC_002947/3189140‑3189420
                                                                                                                                          |                                                                                                                                              
atgatgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATACAGCCTTTGCTCACAGCACCTGCTGTGAg                                                                                                                                      <  6:209708/149‑1 (MQ=255)
 tgatgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAgc                                                                                                                                     <  5:257416/149‑1 (MQ=255)
 tgatgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCAAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAgc                                                                                                                                     <  3:88831/149‑1 (MQ=255)
          ccATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAAt                                                                                                                            <  3:54217/149‑1 (MQ=255)
                   cGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGt                                                                                                                   >  2:43036/1‑149 (MQ=255)
                              aCGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGAAGGTGCTGTGAGCTCGCCCAATCCGTCGCGTCAGCCAGCGTc                                                                                                        >  6:96840/1‑149 (MQ=255)
                                 gAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCAACAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGc                                                                                                     <  8:36325/149‑1 (MQ=255)
                                             tCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCt                                                                                          >  5:109476/1‑148 (MQ=255)
                                               gAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGaa                                                                                       <  7:35755/149‑1 (MQ=255)
                                                        atcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCt                                                                               <  2:115551/148‑1 (MQ=255)
                                                        atcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTc                                                                              >  7:20369/1‑149 (MQ=255)
                                                         tcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTc                                                                              >  7:132722/1‑148 (MQ=255)
                                                         tcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTc                                                                              >  5:48710/1‑148 (MQ=255)
                                                          caCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTTTTTTACGTGAGTAGCAATCAGATTCAGCCTTTGCTCACAGCAGATGCAGTGACCGCGCCCAATCCGTCGCTTAAGCCAGCGTACGATAGAAGGCCCTGAAGAACGACTCg                                                                             >  8:86350/1‑148 (MQ=255)
                                                          caCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                            >  3:6089/1‑149 (MQ=255)
                                                           aCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGat                                                                           <  4:6089/149‑1 (MQ=255)
                                                               tgtTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATc                                                                        <  1:43036/148‑1 (MQ=255)
                                                               tgtTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAgcaaa                                                                                                                                  <  3:195711/90‑4 (MQ=39)
                                                               tgtTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAgcaaa                                                                                                                                  >  4:195711/1‑87 (MQ=39)
                                                                    acgcacgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTg                                                                  <  8:20369/149‑1 (MQ=255)
                                                                        acgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGatat                                                                         <  2:242233/138‑1 (MQ=255)
                                                                        acgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGatat                                                                         >  1:242233/1‑138 (MQ=255)
                                                                        acgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGggtg                                                              <  8:87278/149‑1 (MQ=255)
                                                                                  ccTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTACTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACg                                                    >  1:71362/1‑149 (MQ=255)
                                                                                    tCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCTTGGGTGGTAATCAACg                                                    >  6:283130/1‑147 (MQ=255)
                                                                                    tCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACg                                                    >  4:267297/1‑147 (MQ=255)
                                                                                      aTGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggag                                                 <  6:48710/148‑1 (MQ=255)
                                                                                      aTGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggag                                                 >  8:75780/1‑148 (MQ=255)
                                                                                      aTGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACgga                                                  <  6:109476/147‑1 (MQ=255)
                                                                                           tgttgtTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAg                                            >  2:94138/1‑148 (MQ=255)
                                                                                                ttttACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGc                                       >  1:199155/1‑148 (MQ=255)
                                                                                                      gTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAg                                         >  2:245138/1‑140 (MQ=255)
                                                                                                      gTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAg                                         <  1:245138/140‑1 (MQ=255)
                                                                                                      gTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCgtg                                >  3:123948/1‑149 (MQ=255)
                                                                                                                 tCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGgc                     >  1:40060/1‑149 (MQ=255)
                                                                                                                              gcgCTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGc        >  4:48600/3‑149 (MQ=255)
                                                                                                                                  tCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCAt    >  1:115284/1‑149 (MQ=255)
                                                                                                                                    acaGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGGGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGc  >  3:104175/1‑149 (MQ=255)
                                                                                                                                    acaGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGc  <  5:96840/149‑1 (MQ=255)
                                                                                                                                    acaGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGc  <  2:40060/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
ATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATCAGCCCATGC  >  NC_002947/3189140‑3189420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑