Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,199,407 C→G 8.8% A36A (GCG→GCC)  PP_2806 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,199,4070CG 8.8% 163.1 / 4.4 54A36A (GCG→GCC) PP_2806TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (30/19);  new base G (3/2);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCCCACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTGCCGCCGGCCCCTTGGC  >  NC_002947/3199260‑3199555
                                                                                                                                                   |                                                                                                                                                     
ccccACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCg                                                                                                                                                      >  7:274535/1‑149 (MQ=255)
 cccACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGCACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGc                                                                                                                                                     >  6:234605/1‑149 (MQ=255)
  ccACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCg                                                                                                                                                      >  4:81146/1‑147 (MQ=255)
  ccACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATC‑GCTGCGGCAg                                                                                                                                                   >  8:45594/1‑149 (MQ=255)
            cgccgccgTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTTCAACGATGCCAGCGGCGAATCTGCTGCCGAAGC‑GATTCGcc                                                                                                                                         >  8:155866/1‑149 (MQ=255)
             gccgccgTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCg                                                                                                                                        >  3:143585/1‑149 (MQ=255)
              ccgccgTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATC‑GCTGCGGCAGCAGATTCGCCgc                                                                                                                                       <  7:45594/149‑2 (MQ=255)
                gccgTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGt                                                                                                                                     <  3:83287/149‑1 (MQ=255)
                        gcCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGt                                                                                                                             >  7:258589/1‑149 (MQ=255)
                          cTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTg                                                                                                                           <  7:285025/149‑1 (MQ=255)
                          cTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTg                                                                                                                           >  3:119205/1‑149 (MQ=255)
                               ggCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCgg                                                                                                                                       <  5:177251/132‑1 (MQ=255)
                               ggCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCgg                                                                                                                                       >  6:177251/1‑132 (MQ=255)
                                  aCGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCAcccc                                                                                                                   <  3:312285/149‑1 (MQ=255)
                                           aaCGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCcgc                                                                                                          <  2:191693/149‑1 (MQ=255)
                                           aaCGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTCTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCcgc                                                                                                          <  7:170462/149‑1 (MQ=255)
                                               aTTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATc                                                                                                      <  4:119205/149‑1 (MQ=255)
                                                   ttgCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCt                                                                                                  <  6:57416/149‑1 (MQ=255)
                                                      cGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCa                                                                                               >  3:9994/1‑149 (MQ=255)
                                                           tGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGt                                                                                           <  8:258589/148‑1 (MQ=255)
                                                           tGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTg                                                                                          <  5:142186/149‑1 (MQ=255)
                                                            gTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTgg                                                                                         >  7:77959/1‑149 (MQ=255)
                                                                         tCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCAt                                                                                                                 <  7:137929/112‑1 (MQ=255)
                                                                         tCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCAt                                                                                                                 >  8:137929/1‑112 (MQ=255)
                                                                         tCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTc                                                                            >  1:173453/1‑149 (MQ=255)
                                                                           gTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCCCCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTcct                                                                          >  3:53944/1‑149 (MQ=255)
                                                                           gTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGa                                                                                   <  2:71394/140‑1 (MQ=255)
                                                                           gTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGa                                                                                   >  1:71394/1‑140 (MQ=255)
                                                                           gTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTcct                                                                          >  2:303137/1‑149 (MQ=255)
                                                                           gTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTcct                                                                          >  7:36012/1‑149 (MQ=255)
                                                                                 cGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCctgcctgc                                                                    >  3:39152/1‑149 (MQ=255)
                                                                                    aTAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGct                                                                 >  1:250335/1‑149 (MQ=255)
                                                                                       gAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCtt                                                              <  3:81146/149‑1 (MQ=255)
                                                                                                   cTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATcc                                                  >  7:36864/1‑149 (MQ=255)
                                                                                                        tgtgGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGc                                              >  5:29926/1‑148 (MQ=255)
                                                                                                        tgtgGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGc                                              >  5:34606/1‑148 (MQ=255)
                                                                                                               gACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGc                                      <  2:173453/149‑1 (MQ=255)
                                                                                                                     gTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTtct                                 <  2:250335/148‑1 (MQ=255)
                                                                                                                      tGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTc                                                             >  3:168941/1‑119 (MQ=255)
                                                                                                                      tGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTc                                                             <  4:168941/119‑1 (MQ=255)
                                                                                                                      tGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTcc                               <  1:303137/149‑1 (MQ=255)
                                                                                                                        cAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGGCCTGCCTGCGCTCTTCCGCCCCACTCCGCGCCATTGAGCGTTCTACCt                             >  8:99210/1‑149 (MQ=255)
                                                                                                                         aaCGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCtt                            >  3:319152/1‑149 (MQ=255)
                                                                                                                            gATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTc                         >  6:48401/1‑149 (MQ=255)
                                                                                                                            gATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTc                         >  5:191180/1‑149 (MQ=255)
                                                                                                                                ccAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTa                     <  4:143585/149‑1 (MQ=255)
                                                                                                                                  aGCGGCGAATCTGGTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTGTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTAtt                   <  5:234605/149‑1 (MQ=255)
                                                                                                                                   ccggcgAATC‑GCTGCGGCAGCAGATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTg                  >  5:259735/2‑149 (MQ=255)
                                                                                                                                      gcgAATC‑GCTGCGGCAGCAGATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTgcc                <  2:155931/148‑1 (MQ=255)
                                                                                                                                      gcgAATC‑GCTGCGGCAGCAGATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTgcc                >  1:155931/1‑148 (MQ=255)
                                                                                                                                        gAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTgccgcc             >  4:1846/1‑149 (MQ=255)
                                                                                                                                        gAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCGCGATAAAGTCCTGCCTGCGCACTTCAGCACCAATCCGAGCCATTCAGCGTTCTCCCTTTTCGGTATTgccgcc             >  2:69907/1‑149 (MQ=255)
                                                                                                                                              gctgcCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTGCCGCCGGcccc       >  3:211752/1‑149 (MQ=255)
                                                                                                                                                 gcCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTGCCGCCGGCCCCTTg    >  7:133669/1‑149 (MQ=255)
                                                                                                                                                   cGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTGCCGCCGGCCCCTTGGc  <  8:77959/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCCCACCGATTCCGCCGCCGTGGCGCCTGTCGGCACGTGTTGCAACGATTGTTGCGGCATGTTGATCAAGGACTCGTAGACCGCATAGAACAGCTCGTCCTTGGTGTGGAAGACATAGTGCAACGATGCCAGCGGCGAATCTGCTGCCGCAGC‑GATTCGCCGGGTTGTGGCGTTGGCCACCCCATGTTCCGCGATCACCTTCACGGTGGCCTCGATAAAGTCCTGCCTGCGCTCTTCAGCACCAATCCGAGCCATTGAGCGTTCTCCCTTTTCGGTATTGCCGCCGGCCCCTTGGC  >  NC_002947/3199260‑3199555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑